EK194

Human C-Reactive Protein ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CRP, C-reactive protein, PTX1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:0.44 pg/mL
  • 检测范围:62.5 pg/mL - 4000 pg/mL
  • 回收率:93% - 107%

在售SKU:70-EK194-48, 70-EK194-96


描述

商品名

Human C-Reactive Protein/CRP ELISA Kit (人C-反应蛋白 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.9% - 5.5%;板间变异系数:3.0% - 5.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

0.44 pg/mL

检测范围

62.5 pg/mL - 4000 pg/mL

回收率

93% - 107%

平均回收率

1

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗 CRP单克隆抗体的96孔聚苯乙烯酶标板
  • 人 CRP冻干标准品
  • CRP检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CRP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CRP与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CRP的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human C-Reactive Protein/CRP ELISA Kit (人C-反应蛋白 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CRP, C-reactive protein, PTX1 靶点信息

CRP 分子靶点信息概述

  • 分子名:CRP, C-reactive protein
  • 基因家族:Short pentraxins
  • 别名:PTX1
  • 全称:pentraxin 1; C-reactive protein, pentraxin-related

CRP 分子靶点综述

C-反应蛋白(CRP)是在血浆中发现的一种环状、五聚体蛋白。肝脏对巨噬细胞和脂肪细胞释放的因子产生应答后可合成CRP。CRP是一种肝源性的急性蛋白,在巨噬细胞和T细胞分泌白介素6后增加,其生理作用是与死细胞或濒死细胞(和某些类型的细菌)表面的溶血磷脂胆碱结合,以通过C1Q复合物来激活补体系统。它与C-肽(胰岛素)或蛋白C(血凝)不相关。CRP通常作为炎症标志物,当机体受到炎症感染时,CRP水平会上升。最近的研究表明,患者具有较高基础水平的CRP,通常伴随着一个较高糖尿病、高血压和心血管疾病的发生率。

人 Human CRP 分子靶点信息

  • 分子名:CRP, C-reactive protein
  • 别称:
    • C-reactive protein, pentraxin-related
    • c-reactive protein, petaxin related
    • MGC149895
    • MGC88244
    • pentraxin 1
    • PTX1
  • 基因序列:NCBI_Gene: 1401
  • 蛋白序列:UniProtKB: P02741

人 Human CRP靶点分子功能(预测)

Enables several functions, including complement component C1q complex binding activity; low-density lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including negative regulation of macrophage derived foam cell differentiation; positive regulation of superoxide anion generation; and regulation of gene expression. Acts upstream of or within vasoconstriction. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); autoimmune disease (multiple); eye disease (multiple); and lung disease (multiple).

操作步骤


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  4. Single-cell transcriptome reveals highly complement activated microglia cells in association with pediatric tuberculous meningitis 

  5. Importance of AIM2 as a serum marker for reflecting severity and predicting a poor outcome of human severe traumatic brain injury: A prospective longitudinal cohort study 

  6. Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier 

  7. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  8. Analysis of Therapeutic Effect and Influencing Factors of Ultrasound-Guided Radiofrequency Ablation in the Treatment of Papillary Thyroid Microcarcinoma 

  9. Establishment of a Rat Model of Chronic Prostatitis/Chronic Pelvic Pain Syndrome (Cp/Cpps) Induced by Immunization with a Novel Peptide T2 

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