EK194

Human C-Reactive Protein ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CRP, C-reactive protein, PTX1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:0.44 pg/mL
  • 检测范围:62.5 pg/mL - 4000 pg/mL
  • 回收率:93% - 107%

在售SKU:70-EK194-48, 70-EK194-96


描述

商品名

Human C-Reactive Protein/CRP ELISA Kit (人C-反应蛋白 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.9% - 5.5%;板间变异系数:3.0% - 5.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

0.44 pg/mL

检测范围

62.5 pg/mL - 4000 pg/mL

回收率

93% - 107%

平均回收率

1

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗 CRP单克隆抗体的96孔聚苯乙烯酶标板
  • 人 CRP冻干标准品
  • CRP检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CRP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CRP与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CRP的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human C-Reactive Protein/CRP ELISA Kit (人C-反应蛋白 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CRP, C-reactive protein, PTX1 靶点信息

CRP 分子靶点信息概述

  • 分子名:CRP, C-reactive protein
  • 基因家族:Short pentraxins
  • 别名:PTX1
  • 全称:pentraxin 1; C-reactive protein, pentraxin-related

CRP 分子靶点综述

C-反应蛋白(CRP)是在血浆中发现的一种环状、五聚体蛋白。肝脏对巨噬细胞和脂肪细胞释放的因子产生应答后可合成CRP。CRP是一种肝源性的急性蛋白,在巨噬细胞和T细胞分泌白介素6后增加,其生理作用是与死细胞或濒死细胞(和某些类型的细菌)表面的溶血磷脂胆碱结合,以通过C1Q复合物来激活补体系统。它与C-肽(胰岛素)或蛋白C(血凝)不相关。CRP通常作为炎症标志物,当机体受到炎症感染时,CRP水平会上升。最近的研究表明,患者具有较高基础水平的CRP,通常伴随着一个较高糖尿病、高血压和心血管疾病的发生率。

人 Human CRP 分子靶点信息

  • 分子名:CRP, C-reactive protein
  • 别称:
    • C-reactive protein, pentraxin-related
    • c-reactive protein, petaxin related
    • MGC149895
    • MGC88244
    • pentraxin 1
    • PTX1
  • 基因序列:NCBI_Gene: 1401
  • 蛋白序列:UniProtKB: P02741

人 Human CRP靶点分子功能(预测)

Enables several functions, including complement component C1q complex binding activity; low-density lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including negative regulation of macrophage derived foam cell differentiation; positive regulation of superoxide anion generation; and regulation of gene expression. Acts upstream of or within vasoconstriction. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); autoimmune disease (multiple); eye disease (multiple); and lung disease (multiple).

操作步骤

ELISA操作常见问题

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引用文献


  1. Short-term efficacy, safety and survival of laparoscopic radical resection for colorectal cancer patients with bowel obstruction 

  2. Effects of the Chinese herbal medicine Hong Huang decoction, on myocardial injury in breast cancer patients who underwent anthracycline-based chemotherapy 

  3. Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot 

  4. Single-cell transcriptome reveals highly complement activated microglia cells in association with pediatric tuberculous meningitis 

  5. Importance of AIM2 as a serum marker for reflecting severity and predicting a poor outcome of human severe traumatic brain injury: A prospective longitudinal cohort study 

  6. Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier 

  7. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  8. Analysis of Therapeutic Effect and Influencing Factors of Ultrasound-Guided Radiofrequency Ablation in the Treatment of Papillary Thyroid Microcarcinoma 

  9. Establishment of a Rat Model of Chronic Prostatitis/Chronic Pelvic Pain Syndrome (Cp/Cpps) Induced by Immunization with a Novel Peptide T2 

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