Human CCL11/Eotaxin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CCL11, Eotaxin, SCYA11
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:3.14 pg/mL
- 检测范围:15.63 pg/mL - 1000 pg/mL
- 回收率:82% - 107%
在售SKU:70-EK1130-48, 70-EK1130-96
描述
商品名 |
Human CCL11/Eotaxin ELISA Kit (人趋化因子配体11 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:4.5% - 6.5%;板间变异系数:5.9% - 8.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
3.14 pg/mL |
检测范围 |
15.63 pg/mL - 1000 pg/mL |
回收率 |
82% - 107% |
平均回收率 |
0.9 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL11抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CCL11与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL11的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CCL11 分子靶点信息概述
- 分子名:CCL11, C-C motif chemokine ligand 11
- 基因家族:Chemokine ligands
- 别名:eotaxin; MGC22554
- 曾用名:SCYA11
- 全称:eotaxin-1; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); chemokine (C-C motif) ligand 11
CCL11 分子靶点综述
趋化因子配体11(CCL11)也称为嗜酸性粒细胞趋化因子(Eotaxin),是CC趋化因子家族中的小分子量细胞因子。Eotaxin通过诱导嗜酸性粒细胞的趋化性进行选择性招募,因此参与变态反应。Eotaxin在人和小鼠血浆中的表达升高与其衰老有关。相比于曾经吸食大麻者和从未吸食大麻者,目前仍在吸食大麻者的血浆中含有更高浓度的Eotaxin。精神分裂者体内的Eotaxin浓度也更高,而大麻正是已知的精神分裂症诱导剂。
人 Human CCL11 分子靶点信息
- 分子名:CCL11, C-C motif chemokine ligand 11
- 别称:
- C-C motif chemokine 11
- chemokine (C-C motif) ligand 11
- eosinophil chemotactic protein
- eotaxin
- eotaxin-1
- MGC22554
- SCYA11
- small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
- small-inducible cytokine A11
- 基因序列:NCBI_Gene: 6356
- 蛋白序列:UniProtKB: P51671
人 Human CCL11靶点分子功能(预测)
Enables CCR3 chemokine receptor binding activity; chemokine activity; and protein dimerization activity. Involved in several processes, including eosinophil chemotaxis; positive regulation of GTPase activity; and positive regulation of actin filament polymerization. Located in extracellular region. Implicated in asthma; human immunodeficiency virus infectious disease; and hyperglycemia. Biomarker of several diseases, including cystic fibrosis; hyperglycemia; lung disease (multiple); nephrotic syndrome type 1; and ureteral obstruction.
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Knockdown of Cadherin 26 Prevents the Inflammatory Responses of Allergic Rhinitis 
- Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 
- Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling 
- Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis 
- NAMPT/SIRT1 Expression Levels in White Blood Cells Differentiate the Different Rheumatoid Arthritis Subsets: An Inspiration from Traditional Chinese Medicine 
- Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway 
Knockdown of Cadherin 26 Prevents the Inflammatory Responses of Allergic Rhinitis 
Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 
Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling 
Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis 
NAMPT/SIRT1 Expression Levels in White Blood Cells Differentiate the Different Rheumatoid Arthritis Subsets: An Inspiration from Traditional Chinese Medicine 
Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway 
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