EK1127

Human BDNF ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:BDNF, neurotrophin
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:1.41 pg/mL
  • 检测范围:6.25 pg/mL - 400 pg/mL
  • 回收率:85% - 115%

在售SKU:70-EK1127-48, 70-EK1127-96


描述

商品名

Human BDNF ELISA Kit (人脑源性神经营养因子 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:5.6% - 6.1%;板间变异系数:2.9% - 7.9%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

1.41 pg/mL

检测范围

6.25 pg/mL - 400 pg/mL

回收率

85% - 115%

平均回收率

1.01

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗BDNF单克隆抗体的96孔聚苯乙烯酶标板
  • 人BDNF冻干标准品
  • BDNF检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人BDNF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的BDNF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中BDNF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human BDNF ELISA Kit (人脑源性神经营养因子 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 BDNF, neurotrophin 靶点信息

BDNF 分子靶点信息概述

  • 分子名:BDNF, brain derived neurotrophic factor
  • 基因家族:Neurotrophins
  • 全称:neurotrophin

BDNF 分子靶点综述

脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。

人 Human BDNF 分子靶点信息

  • 分子名:BDNF, brain derived neurotrophic factor
  • 别称:
    • abrineurin
    • ANON2
    • brain-derived neurotrophic factor
    • BULN2
    • MGC34632
    • neurotrophin
  • 基因序列:NCBI_Gene: 627
  • 蛋白序列:UniProtKB: P23560

人 Human BDNF靶点分子功能(预测)

Predicted to enable growth factor activity and nerve growth factor receptor binding activity. Involved in nervous system development; positive regulation of nervous system development; and positive regulation of receptor binding activity. Located in mitochondrion and nuclear speck. Implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Biomarker of several diseases, including autoimmune disease (multiple); lung disease (multiple); mental depression (multiple); neurodegenerative disease (multiple); and open-angle glaucoma (multiple).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy 

  2. A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 

  3. Peripheral Blood Circular RNAs as a Biomarker for Major Depressive Disorder and Prediction of Possible Pathways 

  4. Analgesic Effects of Directed Repetitive Transcranial Magnetic Stimulation in Acute Neuropathic Pain After Spinal Cord Injury 

  5. The effect of bilateral low-frequency rTMS over dorsolateral prefrontal cortex on serum brain-derived neurotropic factor and serotonin in patients with generalized anxiety disorder 

  6. High-efficiency brain-targeted intranasal delivery of BDNF mediated by engineered exosomes to promote remyelination 

  7. Correlation between nocturnal intermittent hypoxemia and mild cognitive impairment in the older adult and the role of BDNF Val66Met polymorphism: a hospital-based cross-sectional study 

  8. The Effect of sequential bilateral low-frequency rTMS over dorsolateral prefrontal cortex on serum level of BDNF and GABA in patients with primary insomnia 

  9. Effects of Training with a Brain–Computer Interface-Controlled Robot on Rehabilitation Outcome in Patients with Subacute Stroke: A Randomized Controlled Trial 

  10. Analgesic Effects of Navigated Repetitive Transcranial Magnetic Stimulation in Patients With Acute Central Poststroke Pain 

  11. Using Dual-Target rTMS, Single-Target rTMS, or Sham rTMS on Post-Stroke Cognitive Impairment 

  12. PM2.5 exposure upregulates pro-inflammatory protein expression in human microglial cells via oxidant stress and TLR4/NF-κB pathway 

  13. Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis 

  14. Potential salivary and serum biomarkers for burning mouth syndrome and their relationship with anxiety/depression 

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