EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥2,190.00 – ¥3,650.00
- 分子靶点:TNF, TNFA, TNFSF2
- 种属:人 (Human)
- 样本类型:血清,血浆
- 检测样本体积:10 μL
- 灵敏度:1.77 pg/mL
- 检测范围:3.13 pg/mL - 200 pg/mL
- 回收率:100% - 122%
在售SKU: 70-EK182EGA-48, 70-EK182EGA-96, EK182EGA, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒
描述
商品名 |
EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法) |
---|---|
检测方法 |
一步法 |
精密度 |
板内变异系数:4.2% - 5.4%;板间变异系数:5.5% - 6.2% |
样本类型 |
血清,血浆 |
检测样本体积 |
10 μL |
灵敏度 |
1.77 pg/mL |
检测范围 |
3.13 pg/mL - 200 pg/mL |
回收率 |
100% - 122% |
平均回收率 |
1.08 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,2-8℃保存。已拆开,标准品-20℃保存,其它2-8℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。
分子信息
TNF 分子靶点信息概述
- 分子名:TNF, tumor necrosis factor
- 基因家族:Tumor necrosis factor superfamily
- 别名:TNFSF2; DIF; TNF-alpha
- 曾用名:TNFA
- 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)
TNF 分子靶点综述
肿瘤坏死因子α (TNF-α),也称为恶病质素和 TNFSF1A,是一种脂肪因子,参与全身的炎症,同时也是刺激急性期反应的细胞因子之一。它主要由活化的巨噬细胞产生,也可由其它类型的细胞分泌,如 CD4+淋巴细胞、NK 细胞、中性粒细胞、肥大细胞、嗜酸性粒细胞和神经元等。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物 (感染因子或组织受损) 产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α在关节组织和其它组织发生炎症的发病机制具有重要作用。最近,越来越多的信息表明 TNF-α也参与了 AIDS 的发病机制。TNF-α 的测定对于移植研究也非常有用。
小鼠 Mouse Tnf 分子靶点信息
- 分子名:Tnf, tumor necrosis factor
- 别称:
- DIF
- TNF alpha
- TNF-alpha
- Tnfa
- TNFalpha
- Tnfsf1a
- tumor necrosis factor-alpha
- tumor necrosis factor, alpha
- 基因序列:NCBI_Gene: 21926
- 蛋白序列:
小鼠 Mouse Tnf 靶点分子功能(预测)
Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).
操作步骤
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引用文献
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Extraction, separation and efficacy of yam polysaccharide 
Metabolic Reprogramming of CD4+ T Cells by Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuates Autoimmune Hepatitis Through Mitochondrial Protein Transfer 
A nanobody-guided multifunctional T cell engager promotes strong anti-tumor responses via synergistic immuno-photothermal effects 
Silencing ARL11 relieved atherosclerotic inflammation and lipid deposition via retraining JAK2/STAT1 pathway 
Negative impact of maternal depressive symptoms on infancy neurodevelopment: a moderated mediation effect of maternal inflammation 
Spatio-temporally controlled all-in-one hydrogel microspheres for ulcerative colitis treatment based on cocktail strategy 
Invasion of human dental pulp fibroblasts by Porphyromonas gingivalis leads to autophagy via the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling pathway 
A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation 
A citrullinated antigenic vaccine in treatment of autoimmune arthritis 
Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer 
The protective role of Cordyceps cicadae and its active ingredient myriocin against sodium iodate-induced age-related macular degeneration via an anti-necroptotic TNF-RIPK1/3 pathway 
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