Easy Go!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥2,190.00 – ¥3,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:TNF, TNFA, TNFSF2
- 种属:大鼠 (Rat)
- 样本类型:细胞培养上清
- 检测样本体积:100 μL
- 灵敏度:0.47 pg/mL
- 检测范围:7.81 - 500 pg/mL
- 回收率:85%-114%
在售SKU: 70-EK382EGB-48, 70-EK382EGB-96, EK382EGB, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒
描述
商品名 |
Easy Go!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法) |
---|---|
检测方法 |
一步法 |
精密度 |
板内变异系数:5.3% - 8.2%;板间变异系数:4.5 % - 7.3% |
样本类型 |
细胞培养上清 |
检测样本体积 |
100 μL |
灵敏度 |
0.47 pg/mL |
检测范围 |
7.81 - 500 pg/mL |
回收率 |
85% - 114% |
平均回收率 |
101% |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,2-8℃保存。已拆开,标准品-20℃保存,其它2-8℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入生物素标记的微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450 nm波长 (参考波长570 - 630 nm) 测定吸光度值。
分子信息
TNF 分子靶点信息概述
- 分子名:TNF, tumor necrosis factor
- 基因家族:Tumor necrosis factor superfamily
- 别名:TNFSF2; DIF; TNF-alpha
- 曾用名:TNFA
- 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)
TNF 分子靶点综述
TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。
大鼠 Rat Tnf 分子靶点信息
- 分子名:Tnf, tumor necrosis factor
- 别称:
- cachectin
- LOC103694380
- MGC124630
- RATTNF
- TNF-a
- TNF-alpha
- Tnfa
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor superfamily member 2
- tumor necrosis factor superfamily, member 2
- tumor necrosis factor-alpha
- tumor necrosis factor-like
- 基因序列:NCBI_Gene: 24835
- 蛋白序列:UniProtKB: P16599
大鼠 Rat Tnf 靶点分子功能(预测)
Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).
操作步骤
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