Cell Cycle Staining Kit 细胞周期检测试剂盒
¥390.00
在售SKU:70-CCS012, 细胞周期, 周期试剂盒, 细胞周期试剂盒, 周期, 细胞周期检测, 试剂盒
描述
产品简介
碘化丙啶(Propidium iodide, PI)可插入碱基之间而与DNA结合,其对碱基几乎没有序列偏好,每4 - 5个碱基对可结合一个染料分子。PI也能与RNA结合,需要用核酸酶处理来区分RNA和DNA染色。一旦染料与核酸结合,其荧光会增强20 - 30倍。虽然其摩尔吸收率(吸光系数)相对较低,PI仍然具有较大的stokes位移,从而在滤光片选择恰当的情况下,仍能同时检测细胞核DNA和荧光标记的抗体。PI可用于荧光显微镜、激光共聚焦、流式细胞仪和荧光计。
PI为膜不通透性,被活细胞排斥,可用于鉴定死细胞和多色荧光复染。下面提供的操作步骤与很多细胞标记技术兼容,如直接或间接抗体检测技术、mRNA原位杂交、特异性的细胞结构染料等。在组织染色中可能要作修改。
本试剂盒包含50 ml DNA染色液和500 μl破膜剂,足够用于50次检测。
产品特点
- 即用型,操作简单
- 活细胞/固定细胞检测
- 常备现货
引用文献
文章目录[隐藏]
- Long non‑coding RNA 01614 hyperactivates WNT/β‑catenin signaling to promote pancreatic cancer progression by suppressing GSK‑3β 
- MicroRNA bta-miR-365-3p inhibits proliferation but promotes differentiation of primary bovine myoblasts by targeting the activin A receptor type I 
- A cold-water polysaccharide-protein complex from Grifola frondosa exhibited antiproliferative activity via mitochondrial apoptotic and Fas/FasL pathways in HepG2 cells 
- Novel Affibody Molecules Targeting the HPV16 E6 Oncoprotein Inhibited the Proliferation of Cervical Cancer Cells 
- PLCA8 suppresses breast cancer apoptosis by activating the PI3k/AKT/NF-κB pathway 
- Overexpression of COPS3 promotes clear cell renal cell carcinoma progression via regulation of Phospho-AKT(Thr308), Cyclin D1 and Caspase-3 
- 25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway 
- The role of miR-431-5p in regulating pulmonary surfactant expression in vitro 
- ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/β-Catenin Signaling 
- NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest 
- CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells 
- Hsa_circRNA_101036 acts as tumor-suppressor in oral squamous cell carcinoma cells via inducing endoplasmic reticulum stress 
- Ureaplasma spp. lipid-associated membrane proteins induce human monocyte U937 cell cycle arrest through p53-independent p21 pathway 
- Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways 
- Astaxanthin Prevents Tuberculosis-Associated Inflammatory Injury by Inhibiting the Caspase 4/11-Gasdermin-Pyroptosis Pathway 
- The long noncoding RNA H19 promotes tamoxifen resistance in breast cancer via autophagy 
- Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease 
- Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 
- Characterization of the release and biological significance of cell-free DNA from breast cancer cell lines 
- WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation 
- MicroRNA-141 inhibits tumor growth and minimizes therapy resistance in colorectal cancer 
- Hydroxychloroquine sensitizes chronic myeloid leukemia cells to Vγ9Vδ2 T cell-mediated lysis independent of autophagy 
- S100P is Overexpressed in Squamous Cell and Adenosquamous Carcinoma Subtypes of Endometrial Cancer and Promotes Cancer Cell Proliferation and Invasion 
- Exosomes derived from miR-126-3p-overexpressing synovial fibroblasts suppress chondrocyte inflammation and cartilage degradation in a rat model of osteoarthritis 
- Resveratrol Suppresses Growth and Migration of Myelodysplastic Cells by Inhibiting the Expression of Elevated Cyclin D1 (CCND1) 
- MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis 
- MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway 
- STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis 
- Low expression of long noncoding RNA CASC2 indicates a poor prognosis and promotes tumorigenesis in thyroid carcinoma 
- MicroRNA‑328 directly targets p21‑activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity 
- Spliceosome-associated factor CTNNBL1 promotes proliferation and invasion in ovarian cancer 
- Knockdown of miR-629 Inhibits Ovarian Cancer Malignant Behaviors by Targeting Testis-Specific Y-Like Protein 5 
- miRNA-30a functions as a tumor suppressor by downregulating cyclin E2 expression in castration-resistant prostate cancer 
- Clinical, cellular, and bioinformatic analyses reveal involvement of WRAP53 overexpression in carcinogenesis of lung adenocarcinoma 
- Downregulation of RUVBL1 inhibits proliferation of lung adenocarcinoma cells by G1/S phase cell cycle arrest via multiple mechanisms 
- A novel lncRNA TCLlnc1 promotes peripheral T cell lymphoma progression through acting as a modular scaffold of HNRNPD and YBX1 complexes 
- Clinical Significance of Long Non-coding RNA MALAT1 Expression in Tissue and Serum of Breast Cancer 
- Long noncoding RNA FEZF1-AS1 indicates a poor prognosis of gastric cancer and promotes tumorigenesis via activation of Wnt signaling pathway 
- Upregulation of long non-coding RNA HOXA-AS2 promotes proliferation and induces epithelial-mesenchymal transition in gallbladder carcinoma 
- E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability 
- Shikonin overcomes drug resistance and induces necroptosis by regulating the miR-92a-1-5p/MLKL axis in chronic myeloid leukemia 
- THBS2 is a Potential Prognostic Biomarker in Colorectal Cancer 
- Generation of affibody molecules specific for HPV16 E7 recognition 
- Neferine inhibits proliferation and collagen synthesis induced by high glucose in cardiac fibroblasts and reduces cardiac fibrosis in diabetic mice 
- Functional mechanism of the enhancement of 5‑fluorouracil sensitivity by TUSC4 in colon cancer cells 
- Hsa_circ_0008934 promotes the proliferation and migration of osteosarcoma cells by targeting miR-145-5p to enhance E2F3 expression 
- MiR-1231 correlates tumor prognosis and inhibits cell growth in ovarian cancer 
- Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells 
- ANLN, Regulated by SP2, Promotes Colorectal Carcinoma Cell Proliferation via PI3K/AKT and MAPK Signaling Pathway 
- 12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis 
- Targeting hexokinase 2 increases the sensitivity of oxaliplatin by Twist1 in colorectal cancer 
- Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma 
- Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3-mediated N6-methyladenosine modification of ICAM2 mRNA in fibroblast-like synoviocytes 
- Retinoic acid receptor gamma is required for proliferation of pancreatic cancer cells 
- Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway 
- Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system 
- Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process 
- STRA6 exerts oncogenic role in gastric tumorigenesis by acting as a crucial target of miR-873 
- High expression of G-protein signaling modulator 2 in hepatocellular carcinoma facilitates tumor growth and metastasis by activating the PI3K/AKT signaling pathway 
- Xylopins A–F, six rare guaiane dimers with three different connecting modes from Xylopia vielana 
- Vieloplains A-G, seven new guaiane-type sesquiterpenoid dimers from Xylopia vielana 
- S100A16 suppresses the growth and survival of leukaemia cells and correlates with relapse and relapse free survival in adults with Philadelphia chromosome-negative B-cell acute lymphoblastic leukaemia 
- miR-363-3p inhibits tumor growth by targeting PCNA in lung adenocarcinoma 
- Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma 
- CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis 
- PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles 
- Long non-coding RNA00844 inhibits MAPK signaling to suppress the progression of hepatocellular carcinoma by targeting AZGP1 
- The resistant effect of SIRT1 in oxidative stress-induced senescence of rat nucleus pulposus cell is regulated by Akt-FoxO1 pathway 
- PARK2 Suppresses Proliferation and Tumorigenicity in Non-small Cell Lung Cancer 
- Histone H3K36me2 demethylase KDM2A promotes bladder cancer progression through epigenetically silencing RARRES3 
- Liver-enriched activator protein 1 as an isoform of CCAAT/enhancer-binding protein beta suppresses stem cell features of hepatocellular carcinoma 
- AKAP9 Upregulation Predicts Unfavorable Prognosis in Pediatric Acute Myeloid Leukemia and Promotes Stemness Properties via the Wnt/β-Catenin Pathway 
- Clock Gene Bmal1 Disruption in Vascular Smooth Muscle Cells Worsens Carotid Atherosclerotic Lesions 
- TAF‑Iβ deficiency inhibits proliferation and promotes apoptosis by rescuing PP2A and inhibiting the AKT/GSK‑3β pathway in leukemic cells 
- BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis 
- Sustained release of Ganoderma lucidum antitumor drugs using a sandwich structured material prepared by electrospinning 
- Preclinical efficacy and involvement of mTOR signaling in the mechanism of Orf virus against nasopharyngeal carcinoma cells 
- miR-182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro 
- Immunomodulatory effect of thymopentin on lymphocytes from supramammary lymph nodes of dairy cows 
- Pinin promotes tumor progression via activating CREB through PI3K/AKT and ERK/MAPK pathway in prostate cancer 
- Hypoxia Stimulates SUMOylation-Dependent Stabilization of KDM5B 
- HN1L is essential for cell growth and survival during nucleopolyhedrovirus infection in silkworm, Bombyx mori 
- Pifithrin-μ is efficacious against non-small cell lung cancer via inhibition of heat shock protein 70 
- LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR-15b in the endometrial epithelium cells of dairy goat 
- Blockade of the mTOR signaling pathway with rapamycin ameliorates aristolochic acid nephropathy 
- Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L. 
- Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure 
- The miR155HG/miR-185/ANXA2 loop contributes to glioblastoma growth and progression 
- Long non-coding RNA MACC1-AS1 promoted pancreatic carcinoma progression through activation of PAX8/NOTCH1 signaling pathway 
- MiR-206 suppresses the deterioration of intrahepatic cholangiocarcinoma and promotes sensitivity to chemotherapy by inhibiting interactions with stromal CAFs 
- Alkaloid Dimers Isolated from Thalictrum baicalense Have Antitumor Activities 
- Effect of ximenynic acid on cell cycle arrest and apoptosis and COX-1 in HepG2 cells 
- Cuprous oxide nanoparticles trigger ER stress-induced apoptosis by regulating copper trafficking and overcoming resistance to sunitinib therapy in renal cancer 
- N7-methylguanosine tRNA modification promotes tumorigenesis and chemoresistance through WNT/β-catenin pathway in nasopharyngeal carcinoma 
- Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells. 
- TRIP13 interference inhibits the proliferation and metastasis of thyroid cancer cells through regulating TTC5/p53 pathway and epithelial-mesenchymal transition related genes expression 
- The Combined Use of Orf Virus and PAK4 Inhibitor Exerts Anti-tumor Effect in Breast Cancer 
- Bone marrow mesenchymal stromal cells affect the cell cycle arrest effect of genotoxic agents on acute lymphocytic leukemia cells via p21 down-regulation 
- Polystyrene micro- and nano-particle coexposure injures fetal thalamus by inducing ROS-mediated cell apoptosis 
- Curcusone C induces telomeric DNA-damage response in cancer cells through inhibition of telomeric repeat factor 2 
- The effects of SAHA on radiosensitivity in pancreatic cancer cells by inducing apoptosis and targeting RAD51 
- miR-148a-3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6 
- ΔFosB regulates rosiglitazone-induced milk fat synthesis and cell survival 
- A MYBL2 complex for RRM2 transactivation and the synthetic effect of MYBL2 knockdown with WEE1 inhibition against colorectal cancer 
- Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines 
- PMEPA1 induces EMT via a non-canonical TGF-β signalling in colorectal cancer 
- Prometryn induces apoptotic cell death through cell cycle arrest and oxidative DNA damage 
- Design, synthesis, and bioactivity study on Lissodendrins B derivatives as PARP1 inhibitor 
- Immunosuppressive activity of pogostone on T cells: Blocking proliferation via S phase arrest 
- The YY1/miR-548t-5p/CXCL11 signaling axis regulates cell proliferation and metastasis in human pancreatic cancer 
- Baicalein inhibits the growth of oral squamous cell carcinoma cells by downregulating the expression of transcription factor Sp1 
- miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development 
- circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a 
- MiR-520d-3p antitumor activity in human breast cancer via post-transcriptional regulation of spindle and kinetochore associated 2 expression 
- The novel long noncoding RNA u50535 promotes colorectal cancer growth and metastasis by regulating CCL20 
- Long non‑coding RNA maternally expressed gene 3 affects cell proliferation, apoptosis and migration by targeting the microRNA‑9‑5p/midkine axis and activating the phosphoinositide‑dependent kinase/AKT pathway in hepatocellular carcinoma 
- Long non-coding RNA AC026166.2-001 inhibits cell proliferation and migration in laryngeal squamous cell carcinoma by regulating the miR-24-3p/p27 axis 
- miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression 
- SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells 
- MiR-449a regulates caprine endometrial stromal cell apoptosis and endometrial receptivity 
Long non‑coding RNA 01614 hyperactivates WNT/β‑catenin signaling to promote pancreatic cancer progression by suppressing GSK‑3β 
MicroRNA bta-miR-365-3p inhibits proliferation but promotes differentiation of primary bovine myoblasts by targeting the activin A receptor type I 
A cold-water polysaccharide-protein complex from Grifola frondosa exhibited antiproliferative activity via mitochondrial apoptotic and Fas/FasL pathways in HepG2 cells 
Novel Affibody Molecules Targeting the HPV16 E6 Oncoprotein Inhibited the Proliferation of Cervical Cancer Cells 
PLCA8 suppresses breast cancer apoptosis by activating the PI3k/AKT/NF-κB pathway 
Overexpression of COPS3 promotes clear cell renal cell carcinoma progression via regulation of Phospho-AKT(Thr308), Cyclin D1 and Caspase-3 
25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway 
The role of miR-431-5p in regulating pulmonary surfactant expression in vitro 
ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/β-Catenin Signaling 
NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest 
CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells 
Hsa_circRNA_101036 acts as tumor-suppressor in oral squamous cell carcinoma cells via inducing endoplasmic reticulum stress 
Ureaplasma spp. lipid-associated membrane proteins induce human monocyte U937 cell cycle arrest through p53-independent p21 pathway 
Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways 
Astaxanthin Prevents Tuberculosis-Associated Inflammatory Injury by Inhibiting the Caspase 4/11-Gasdermin-Pyroptosis Pathway 
The long noncoding RNA H19 promotes tamoxifen resistance in breast cancer via autophagy 
Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease 
Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 
Characterization of the release and biological significance of cell-free DNA from breast cancer cell lines 
WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation 
MicroRNA-141 inhibits tumor growth and minimizes therapy resistance in colorectal cancer 
Hydroxychloroquine sensitizes chronic myeloid leukemia cells to Vγ9Vδ2 T cell-mediated lysis independent of autophagy 
S100P is Overexpressed in Squamous Cell and Adenosquamous Carcinoma Subtypes of Endometrial Cancer and Promotes Cancer Cell Proliferation and Invasion 
Exosomes derived from miR-126-3p-overexpressing synovial fibroblasts suppress chondrocyte inflammation and cartilage degradation in a rat model of osteoarthritis 
Resveratrol Suppresses Growth and Migration of Myelodysplastic Cells by Inhibiting the Expression of Elevated Cyclin D1 (CCND1) 
MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis 
MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway 
STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis 
Low expression of long noncoding RNA CASC2 indicates a poor prognosis and promotes tumorigenesis in thyroid carcinoma 
MicroRNA‑328 directly targets p21‑activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity 
Spliceosome-associated factor CTNNBL1 promotes proliferation and invasion in ovarian cancer 
Knockdown of miR-629 Inhibits Ovarian Cancer Malignant Behaviors by Targeting Testis-Specific Y-Like Protein 5 
miRNA-30a functions as a tumor suppressor by downregulating cyclin E2 expression in castration-resistant prostate cancer 
Clinical, cellular, and bioinformatic analyses reveal involvement of WRAP53 overexpression in carcinogenesis of lung adenocarcinoma 
Downregulation of RUVBL1 inhibits proliferation of lung adenocarcinoma cells by G1/S phase cell cycle arrest via multiple mechanisms 
A novel lncRNA TCLlnc1 promotes peripheral T cell lymphoma progression through acting as a modular scaffold of HNRNPD and YBX1 complexes 
Clinical Significance of Long Non-coding RNA MALAT1 Expression in Tissue and Serum of Breast Cancer 
Long noncoding RNA FEZF1-AS1 indicates a poor prognosis of gastric cancer and promotes tumorigenesis via activation of Wnt signaling pathway 
Upregulation of long non-coding RNA HOXA-AS2 promotes proliferation and induces epithelial-mesenchymal transition in gallbladder carcinoma 
E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability 
Shikonin overcomes drug resistance and induces necroptosis by regulating the miR-92a-1-5p/MLKL axis in chronic myeloid leukemia 
THBS2 is a Potential Prognostic Biomarker in Colorectal Cancer 
Generation of affibody molecules specific for HPV16 E7 recognition 
Neferine inhibits proliferation and collagen synthesis induced by high glucose in cardiac fibroblasts and reduces cardiac fibrosis in diabetic mice 
Functional mechanism of the enhancement of 5‑fluorouracil sensitivity by TUSC4 in colon cancer cells 
Hsa_circ_0008934 promotes the proliferation and migration of osteosarcoma cells by targeting miR-145-5p to enhance E2F3 expression 
MiR-1231 correlates tumor prognosis and inhibits cell growth in ovarian cancer 
Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells 
ANLN, Regulated by SP2, Promotes Colorectal Carcinoma Cell Proliferation via PI3K/AKT and MAPK Signaling Pathway 
12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis 
Targeting hexokinase 2 increases the sensitivity of oxaliplatin by Twist1 in colorectal cancer 
Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma 
Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3-mediated N6-methyladenosine modification of ICAM2 mRNA in fibroblast-like synoviocytes 
Retinoic acid receptor gamma is required for proliferation of pancreatic cancer cells 
Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway 
Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system 
Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process 
STRA6 exerts oncogenic role in gastric tumorigenesis by acting as a crucial target of miR-873 
High expression of G-protein signaling modulator 2 in hepatocellular carcinoma facilitates tumor growth and metastasis by activating the PI3K/AKT signaling pathway 
Xylopins A–F, six rare guaiane dimers with three different connecting modes from Xylopia vielana 
Vieloplains A-G, seven new guaiane-type sesquiterpenoid dimers from Xylopia vielana 
S100A16 suppresses the growth and survival of leukaemia cells and correlates with relapse and relapse free survival in adults with Philadelphia chromosome-negative B-cell acute lymphoblastic leukaemia 
miR-363-3p inhibits tumor growth by targeting PCNA in lung adenocarcinoma 
Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma 
CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis 
PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles 
Long non-coding RNA00844 inhibits MAPK signaling to suppress the progression of hepatocellular carcinoma by targeting AZGP1 
The resistant effect of SIRT1 in oxidative stress-induced senescence of rat nucleus pulposus cell is regulated by Akt-FoxO1 pathway 
PARK2 Suppresses Proliferation and Tumorigenicity in Non-small Cell Lung Cancer 
Histone H3K36me2 demethylase KDM2A promotes bladder cancer progression through epigenetically silencing RARRES3 
Liver-enriched activator protein 1 as an isoform of CCAAT/enhancer-binding protein beta suppresses stem cell features of hepatocellular carcinoma 
AKAP9 Upregulation Predicts Unfavorable Prognosis in Pediatric Acute Myeloid Leukemia and Promotes Stemness Properties via the Wnt/β-Catenin Pathway 
Clock Gene Bmal1 Disruption in Vascular Smooth Muscle Cells Worsens Carotid Atherosclerotic Lesions 
TAF‑Iβ deficiency inhibits proliferation and promotes apoptosis by rescuing PP2A and inhibiting the AKT/GSK‑3β pathway in leukemic cells 
BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis 
Sustained release of Ganoderma lucidum antitumor drugs using a sandwich structured material prepared by electrospinning 
Preclinical efficacy and involvement of mTOR signaling in the mechanism of Orf virus against nasopharyngeal carcinoma cells 
miR-182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro 
Immunomodulatory effect of thymopentin on lymphocytes from supramammary lymph nodes of dairy cows 
Pinin promotes tumor progression via activating CREB through PI3K/AKT and ERK/MAPK pathway in prostate cancer 
Hypoxia Stimulates SUMOylation-Dependent Stabilization of KDM5B 
HN1L is essential for cell growth and survival during nucleopolyhedrovirus infection in silkworm, Bombyx mori 
Pifithrin-μ is efficacious against non-small cell lung cancer via inhibition of heat shock protein 70 
LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR-15b in the endometrial epithelium cells of dairy goat 
Blockade of the mTOR signaling pathway with rapamycin ameliorates aristolochic acid nephropathy 
Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L. 
Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure 
The miR155HG/miR-185/ANXA2 loop contributes to glioblastoma growth and progression 
Long non-coding RNA MACC1-AS1 promoted pancreatic carcinoma progression through activation of PAX8/NOTCH1 signaling pathway 
MiR-206 suppresses the deterioration of intrahepatic cholangiocarcinoma and promotes sensitivity to chemotherapy by inhibiting interactions with stromal CAFs 
Alkaloid Dimers Isolated from Thalictrum baicalense Have Antitumor Activities 
Effect of ximenynic acid on cell cycle arrest and apoptosis and COX-1 in HepG2 cells 
Cuprous oxide nanoparticles trigger ER stress-induced apoptosis by regulating copper trafficking and overcoming resistance to sunitinib therapy in renal cancer 
N7-methylguanosine tRNA modification promotes tumorigenesis and chemoresistance through WNT/β-catenin pathway in nasopharyngeal carcinoma 
Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells. 
TRIP13 interference inhibits the proliferation and metastasis of thyroid cancer cells through regulating TTC5/p53 pathway and epithelial-mesenchymal transition related genes expression 
The Combined Use of Orf Virus and PAK4 Inhibitor Exerts Anti-tumor Effect in Breast Cancer 
Bone marrow mesenchymal stromal cells affect the cell cycle arrest effect of genotoxic agents on acute lymphocytic leukemia cells via p21 down-regulation 
Polystyrene micro- and nano-particle coexposure injures fetal thalamus by inducing ROS-mediated cell apoptosis 
Curcusone C induces telomeric DNA-damage response in cancer cells through inhibition of telomeric repeat factor 2 
The effects of SAHA on radiosensitivity in pancreatic cancer cells by inducing apoptosis and targeting RAD51 
miR-148a-3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6 
ΔFosB regulates rosiglitazone-induced milk fat synthesis and cell survival 
A MYBL2 complex for RRM2 transactivation and the synthetic effect of MYBL2 knockdown with WEE1 inhibition against colorectal cancer 
Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines 
PMEPA1 induces EMT via a non-canonical TGF-β signalling in colorectal cancer 
Prometryn induces apoptotic cell death through cell cycle arrest and oxidative DNA damage 
Design, synthesis, and bioactivity study on Lissodendrins B derivatives as PARP1 inhibitor 
Immunosuppressive activity of pogostone on T cells: Blocking proliferation via S phase arrest 
The YY1/miR-548t-5p/CXCL11 signaling axis regulates cell proliferation and metastasis in human pancreatic cancer 
Baicalein inhibits the growth of oral squamous cell carcinoma cells by downregulating the expression of transcription factor Sp1 
miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development 
circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a 
MiR-520d-3p antitumor activity in human breast cancer via post-transcriptional regulation of spindle and kinetochore associated 2 expression 
The novel long noncoding RNA u50535 promotes colorectal cancer growth and metastasis by regulating CCL20 
Long non‑coding RNA maternally expressed gene 3 affects cell proliferation, apoptosis and migration by targeting the microRNA‑9‑5p/midkine axis and activating the phosphoinositide‑dependent kinase/AKT pathway in hepatocellular carcinoma 
Long non-coding RNA AC026166.2-001 inhibits cell proliferation and migration in laryngeal squamous cell carcinoma by regulating the miR-24-3p/p27 axis 
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression 
SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells 
MiR-449a regulates caprine endometrial stromal cell apoptosis and endometrial receptivity 
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检测步骤
文章目录[隐藏]
- 一、可当天检测的活细胞样本
- 二、当天无法检测的活细胞样本
- 细胞周期检测操作视频
- FCM流式检测常见问题
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- 裂红法vs密度梯度离心法 
- 流式实验图有点难吗~小科教你轻松看懂! 
- 流式非特异性荧光的照妖镜-同型对照 
- 论文分享 | 联科流式抗体助力胶质母细胞瘤的免疫治疗研究 
- 干货分享 | 要想流式图好看,细胞前处理你得好好搞 
- 论文分享 | 流式细胞术神助攻癌症免疫治疗研究新进展 
- 干货分享 | 辅助试剂助力流式检测更高效、稳定完成 
- 干货分享 | 怎么用流式解决细胞周期检测,你会了吗? 
- 基础提炼 | 手牵手,好朋友,流式路上共前进 
- 流式细胞术知识分享 — 数据分析篇 
- 流式细胞术知识分享 — 应用篇 
- 流式细胞术知识分享 — 试剂篇 
- 流式细胞术知识分享 一 仪器篇 
- 流式细胞术之荧光知识 
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- 分享|流式细胞术检测信号及意义 
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- 【视频】Treg检测操作演示 
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- 多色流式中荧光补偿调节方法与步骤 
- 查看更多FCM流式检测相关问题
一、可当天检测的活细胞样本
- 收集2 × 105- 1 × 106个细胞,离心弃上清。用PBS洗涤一次,离心弃上清。
- 加入1 mL DNA Staining Solution和10 μL Permeabilization Solution,涡旋振荡5 - 10秒混匀。室温避光孵育30分钟。
- 选择最低上样速度,在流式细胞仪上进行检测。如需在荧光显微镜下观察,则离心细胞,弃上清,加入新鲜缓冲液重悬。滴加1滴细胞悬液至载玻片,盖上盖玻片后使用合适的滤光片进行观察。
二、当天无法检测的活细胞样本
- 按照下述方法固定,或其他合适的方法固定
- 收集2 × 105- 1 × 106个细胞,离心弃上清。轻弹管壁,使沉淀重悬在残余的液体中,加入1 mL 室温下的PBS。
- 将细胞缓慢加入至3 mL无水乙醇(-20℃预冷)中,边加边高速搅拌。-20℃固定过夜,可保存数月。
- 检测当天,将固定细胞离心,弃去乙醇,轻弹管壁使沉淀松散,加入2 - 5 mL 室温下的PBS,放置15分钟使细胞再次水化。离心,弃上清。
- 加入1 ml DNA Staining Solution,涡旋振荡5 - 10秒混匀。室温避光孵育30分钟。
- 选择最低上样速度,在流式细胞仪上进行检测。如需在荧光显微镜下观察,则离心细胞,弃上清,加入新鲜缓冲液重悬。滴加1滴细胞悬液至载玻片,盖上盖玻片后使用合适的滤光片进行观察。
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