F21008A02

Anti-Mouse CD8α, PE (Clone:2.43) 流式抗体 (新品)

¥208.00 – ¥440.00

说明书

分子靶点：CD8A, CD8
种属：小鼠 (Mouse)
标记：PE
亚型：Rat IgG2b
克隆号：2.43

在售SKU, 70-AM008A0204-20, 70-AM008A0204-50, 70-AM008A0204-100, AM008A0204, 70-F21008A02-25, 70-F21008A02-100

描述

文章目录[隐藏]

结果示例
分子信息
CD8A 分子靶点信息概述
CD8A 分子靶点综述
- 小鼠 Mouse Cd8a 分子靶点信息
- 小鼠 Mouse Cd8a 靶点分子功能（预测）

商品名	Anti-Mouse CD8α, PE (Clone:2.43) 流式抗体
靶点	CD8A, CD8
标记	PE
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Rat IgG2b
克隆号	2.43
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - ICR 小鼠脾细胞染色 Anti-Mouse CD8α, PE (F21008A02) 和 Anti-Mouse CD4, APC (F2100403) 的结果。已排除粘连细胞。

分子信息

概述小鼠 CD8A, CD8 靶点信息

CD8A 分子靶点信息概述

分子名：CD8A, CD8a molecule
基因家族：CD molecules; V-set domain containing
曾用名：CD8
全称：CD8 antigen, alpha polypeptide (p32); T-cell surface glycoprotein CD8 alpha chain

CD8A 分子靶点综述

CD8也称为T8或Leu2，是免疫球蛋白超家族的一员，表达于大部分胸腺细胞、外周血T细胞亚群和NK细胞(基本为CD8α/α同源二聚体)。CD8在抗原识别和T细胞活化中，作为MHC I类分子受限的T细胞受体，并在胸腺分化中具有重要作用。

小鼠 Mouse Cd8a 分子靶点信息

分子名：Cd8a, CD8 antigen, alpha chain
别称：
- BB154331
- expressed sequence BB154331
- Ly-2
- Ly-35
- Ly-B
- lymphocyte antigen 2
- lymphocyte antigen 35
- Lyt-2
- T-lymphocyte antigen 2
基因序列：NCBI_Gene: 12525
蛋白序列：

小鼠 Mouse Cd8a 靶点分子功能（预测）

Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and hemolymphoid system gland. Orthologous to human CD8A (CD8a molecule).

引用文献统计

该产品被引用的文献总数为：25

Combination of matrine and tacrolimus alleviates acute rejection in murine heart transplantation by inhibiting DCs maturation through ROS/ERK/NF-κB pathway
影响因子：4.932刊物：INTERNATIONAL IMMUNOPHARMACOLOGY发表日期：2021/10/19
Novel highly branched water-soluble heteropolysaccharides as immunopotentiators to inhibit S-180 tumor cell growth in BALB/c mice
影响因子：3.463刊物：CARBOHYDRATE POLYMERS发表日期：2011/8/22
Codelivery of Shikonin and siTGF-β for enhanced triple negative breast cancer chemo-immunotherapy
影响因子：11.467刊物：JOURNAL OF CONTROLLED RELEASE发表日期：2022/1/12
Polysaccharide of Radix Pseudostellariae Improves Chronic Fatigue Syndrome Induced by Poly I:C in Mice
影响因子：2.964刊物：Evidence-based Complementary and Alternative Medicine发表日期：2011/6/23
Artesunate Restrains Maturation of Dendritic Cells and Ameliorates Heart Transplantation-Induced Acute Rejection in Mice through the PERK/ATF4/CHOP Signaling Pathway
影响因子：4.711刊物：MEDIATORS OF INFLAMMATION发表日期：2021/8/21
Photothermally activatable PDA immune nanomedicine combined with PD-L1 checkpoint blockade for antimetastatic cancer photoimmunotherapy
影响因子：5.047刊物：Journal of Materials Chemistry B发表日期：2019/3/4
Quaternized chitosan-coated liposomes enhance immune responses by co-delivery of antigens and resveratrol
影响因子：6.51刊物：INTERNATIONAL JOURNAL OF PHARMACEUTICS发表日期：2022/10/12
Evaluation of the immune response induced by DNA vaccines expressing MIF and MCD-1 genes of Trichinella spiralis in BALB/c mice
影响因子：1.544刊物：JOURNAL OF HELMINTHOLOGY发表日期：2011/11/25
A DNA vaccine co-expressing Trichinella spiralis MIF and MCD-1 with murine ubiquitin induces partial protective immunity in mice
影响因子：1.38刊物：JOURNAL OF HELMINTHOLOGY发表日期：2012/1/5
Effect of orally administered bovine colostrum on cytokine production in vivo and in vitro in immunosuppressed mice
影响因子：2.409刊物：INTERNATIONAL DAIRY JOURNAL发表日期：2010/3/6
Anti-tumor and immunomodulatory effects of Grifola frondosa polysaccharide combined with vitamin C on Heps-bearing mice: Based on inducing apoptosis and autophagy
影响因子：4.451刊物：Journal of Functional Foods发表日期：2021/10/25
Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles
影响因子：15.5刊物：Journal of Extracellular Vesicles发表日期：2024-09-24
Astragaloside IV microfibers assembling into injectable 3D-scaffolds with intrinsic immunoactivity for enhanced tumor vaccine efficacy
影响因子：13.3刊物：CHEMICAL ENGINEERING JOURNAL发表日期：2024-09-06
Mitigation of Deoxynivalenol (DON)- and Aflatoxin B1 (AFB1)-Induced Immune Dysfunction and Apoptosis in Mouse Spleen by Curcumin
影响因子：3.9刊物：Toxins发表日期：2024-08-13
Paroxetine alleviates dendritic cell and T lymphocyte activation via GRK2-mediated PI3K–AKT signaling in rheumatoid arthritis
影响因子：7.5刊物：CHINESE MEDICAL JOURNAL发表日期：2024-06-14
No-Observed Adverse Effect Levels of Deoxynivalenol and Aflatoxin B1 in Combination Induced Immune Inhibition and Apoptosis in vivo and in vitro
影响因子：3.9刊物：FOOD AND CHEMICAL TOXICOLOGY发表日期：2024-05-17
From aberrant neurodevelopment to neurodegeneration: Insights into the hub gene associated with autism and alzheimer's disease
影响因子：2.7刊物：BRAIN RESEARCH发表日期：2024-05-08
Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia?reperfusion injury in heart transplantation
影响因子：7.4刊物：Journal of Translational Medicine发表日期：2023-12-20
Lymph node-inspired immunoregulatory hydrogel with siRNA delivery property for postoperative glioblastoma treatment
影响因子：15.1刊物：CHEMICAL ENGINEERING JOURNAL发表日期：2023-10-11
Remodeling tumor immunosuppression with molecularly imprinted nanoparticles to enhance immunogenic cell death for cancer immunotherapy
影响因子：10.8刊物：JOURNAL OF CONTROLLED RELEASE发表日期：2023-08-25
Litchi procyanidins inhibit colon cancer proliferation and metastasis by triggering gut-lung axis immunotherapy
影响因子：9刊物：Cell Death & Disease发表日期：2023-02-11
Chrysanthemum sporopollenin: A novel vaccine delivery system for nasal mucosal immunity
影响因子：7.3刊物：Frontiers in Immunology发表日期：2023-02-09
Selenium Improves Bone Microenvironment-Related Hematopoiesis and Immunity in T-2 Toxin-Exposed Mice
影响因子：6.1刊物：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY发表日期：2023-01-24
Anti-tumor and immunomodulatory effects of Grifola frondosa polysaccharide combined with vitamin C on Heps-bearing mice: Based on inducing apoptosis and autophagy
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Remodeling tumor immunosuppression with molecularly imprinted nanoparticles to enhance immunogenic cell death for cancer immunotherapy
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操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Mouse CD8α, PE 和其他抗体，震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 ×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Mouse CD8α, PE 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。