Anti-Human/Mouse CD11b, FITC (Clone: M1/70) 检测试剂
¥208.00 – ¥440.00
- 分子靶点:CD11B, MAC-1, ITGAM, CR3A
- 种属:人 (Human); 小鼠 (Mouse)
- 标记:FITC
- 亚型:Rat IgG2a, κ
- 克隆号:M1/70
在售SKU, 70-F41011b01-20, 70-F41011b01-50, 70-F41011b01-100, 70-F41011b01-25
描述
文章目录[隐藏]
商品名 |
Anti-Human/Mouse CD11b, FITC (Clone: M1/70) 检测试剂 |
---|---|
靶点 | |
标记 |
FITC |
仪器 |
适用488nm激光器的流式细胞仪 |
亚型 |
Rat IgG2a, κ |
克隆号 |
M1/70 |
推荐应用 |
流式检测 |
用量 |
5 μL /Test |
纯化 |
亲和层析 |
储存液 |
含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2) |
保存条件 |
2 - 8℃避光,切勿冻存 |
结果示例
图 - 人外周血染色,左图为Rat IgG2a, κ Isotype control, FITC的结果,右图为Anti-Human/Mouse CD11b,FITC的结果。以淋巴细胞、粒细胞和单核细胞设门进行分析。
分子信息
ITGAM 分子靶点信息概述
- 分子名:ITGAM, integrin subunit alpha M
- 基因家族:CD molecules; Integrin alpha subunits; Complement system regulators and receptors
- 别名:MAC-1; CD11b
- 曾用名:CR3A; CD11B
- 全称:complement component 3 receptor 3 subunit; macrophage-1 antigen alpha subunit; integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin, alpha M (complement component 3 receptor 3 subunit)
ITGAM 分子靶点综述
整合素αM(ITGAM)是一种形成巨噬细胞抗原(Mac-1)或补体受体3(CR3)的蛋白亚单位。ITGAM又被称为CR3A和分化抗原簇分子11B(CD11b)。CD11b是一种常见的髓系标志物,在急性髓系白血病中有50%的阳性表达率。CD117和CD11b共同检测有助于从恢复良性增殖(CD117阴性表达,CD11b阳性表达)的病例中区分出急性早幼粒细胞白血病(CD11b阴性表达)。
人 Human ITGAM 分子靶点信息
- 分子名:ITGAM, integrin subunit alpha M
- 别称:
- antigen CD11b (p170)
- CD11 antigen-like family member B
- CD11B
- cell surface glycoprotein MAC-1 subunit alpha
- complement component 3 receptor 3 subunit
- CR-3 alpha chain
- CR3A
- integrin alpha-M
- integrin, alpha M (complement component 3 receptor 3 subunit)
- integrin, alpha M (complement component receptor 3, alpha)
- leukocyte adhesion receptor MO1
- MAC-1
- MAC1A
- macrophage antigen alpha polypeptide
- macrophage-1 antigen alpha subunit
- MGC117044
- MO1A
- neutrophil adherence receptor alpha-M subunit
- SLEB6
- 基因序列:NCBI_Gene: 3684
- 蛋白序列:UniProtKB: P11215
人 Human ITGAM靶点分子功能(预测)
Enables heat shock protein binding activity. Involved in ectodermal cell differentiation; positive regulation of neutrophil degranulation; and positive regulation of superoxide anion generation. Located in cell surface; extracellular space; and plasma membrane raft. Implicated in lupus nephritis.
小鼠 Mouse Itgam 分子靶点信息
- 分子名:Itgam, integrin alpha M
- 别称:
- Cd11b
- CD11B (p170)
- CD11b/CD18
- complement component receptor 3 alpha
- complement receptor type 3
- CR3
- F730045J24Rik
- Ly-40
- lymphocyte antigen 40
- Mac-1
- Mac-1 alpha
- Mac-1a
- macrophage antigen alpha chain (integrin)
- RIKEN cDNA F730045J24 gene
- 基因序列:NCBI_Gene: 16409
- 蛋白序列:
小鼠 Mouse Itgam 靶点分子功能(预测)
Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M).
操作步骤
文章目录[隐藏]
- 使用方法
- 注意事项
- FCM流式检测常见问题
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- 查看更多FCM流式检测相关问题
使用方法
全血样本:取100 μl 抗凝血,加入5 μl Anti-Human/Mouse CD11b, FITC 和其他抗体,震荡混匀,于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300×g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号S1001)重悬,加入5 μl Anti-Human CD11b, FITC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。
注意事项
- 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
- 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
- 更多相关产品敬请关注联科生物网站或来电咨询。
FCM流式检测常见问题
查看更多FCM流式检测相关问题
引用文献
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