F41011b01

Anti-Human/Mouse CD11b, FITC (Clone: M1/70) 检测试剂

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¥208.00¥440.00

  • 分子靶点:CD11B, MAC-1, ITGAM, CR3A
  • 种属:人 (Human); 小鼠 (Mouse)
  • 标记:FITC
  • 亚型:Rat IgG2a, κ
  • 克隆号:M1/70

在售SKU, 70-F41011b01-20, 70-F41011b01-50, 70-F41011b01-100, 70-F41011b01-25


描述

商品名

Anti-Human/Mouse CD11b, FITC (Clone: M1/70) 检测试剂

靶点

CD11B, MAC-1, ITGAM, CR3A

标记

FITC

仪器

适用488nm激光器的流式细胞仪

亚型

Rat IgG2a, κ

克隆号

M1/70

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human/Mouse CD11b, FITC (Clone:M1/70)流式抗体 - 结果示例图片Anti-Human/Mouse CD11b, FITC (Clone:M1/70)流式抗体 - 结果示例图片
结果示例

图 - 人外周血染色,左图为Rat IgG2a, κ Isotype control, FITC的结果,右图为Anti-Human/Mouse CD11b,FITC的结果。以淋巴细胞、粒细胞和单核细胞设门进行分析。

分子信息

概述人 Human ITGAM 分子靶点信息小鼠 Mouse Itgam 分子靶点信息

ITGAM 分子靶点信息概述

  • 分子名:ITGAM, integrin subunit alpha M
  • 基因家族:CD molecules; Integrin alpha subunits; Complement system regulators and receptors
  • 别名:MAC-1; CD11b
  • 曾用名:CR3A; CD11B
  • 全称:complement component 3 receptor 3 subunit; macrophage-1 antigen alpha subunit; integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin, alpha M (complement component 3 receptor 3 subunit)

ITGAM 分子靶点综述

整合素αM(ITGAM)是一种形成巨噬细胞抗原(Mac-1)或补体受体3(CR3)的蛋白亚单位。ITGAM又被称为CR3A和分化抗原簇分子11B(CD11b)。CD11b是一种常见的髓系标志物,在急性髓系白血病中有50%的阳性表达率。CD117和CD11b共同检测有助于从恢复良性增殖(CD117阴性表达,CD11b阳性表达)的病例中区分出急性早幼粒细胞白血病(CD11b阴性表达)。

人 Human ITGAM 分子靶点信息

  • 分子名:ITGAM, integrin subunit alpha M
  • 别称:
    • antigen CD11b (p170)
    • CD11 antigen-like family member B
    • CD11B
    • cell surface glycoprotein MAC-1 subunit alpha
    • complement component 3 receptor 3 subunit
    • CR-3 alpha chain
    • CR3A
    • integrin alpha-M
    • integrin, alpha M (complement component 3 receptor 3 subunit)
    • integrin, alpha M (complement component receptor 3, alpha)
    • leukocyte adhesion receptor MO1
    • MAC-1
    • MAC1A
    • macrophage antigen alpha polypeptide
    • macrophage-1 antigen alpha subunit
    • MGC117044
    • MO1A
    • neutrophil adherence receptor alpha-M subunit
    • SLEB6
  • 基因序列:NCBI_Gene: 3684
  • 蛋白序列:UniProtKB: P11215

人 Human ITGAM靶点分子功能(预测)

Enables heat shock protein binding activity. Involved in ectodermal cell differentiation; positive regulation of neutrophil degranulation; and positive regulation of superoxide anion generation. Located in cell surface; extracellular space; and plasma membrane raft. Implicated in lupus nephritis.

小鼠 Mouse Itgam 分子靶点信息

  • 分子名:Itgam, integrin alpha M
  • 别称:
    • Cd11b
    • CD11B (p170)
    • CD11b/CD18
    • complement component receptor 3 alpha
    • complement receptor type 3
    • CR3
    • F730045J24Rik
    • Ly-40
    • lymphocyte antigen 40
    • Mac-1
    • Mac-1 alpha
    • Mac-1a
    • macrophage antigen alpha chain (integrin)
    • RIKEN cDNA F730045J24 gene
  • 基因序列:NCBI_Gene: 16409
  • 蛋白序列:

小鼠 Mouse Itgam 靶点分子功能(预测)

Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M).

操作步骤

使用方法

全血样本:取100 μl 抗凝血,加入5 μl Anti-Human/Mouse CD11b, FITC 和其他抗体,震荡混匀,于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300×g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号S1001)重悬,加入5 μl Anti-Human CD11b, FITC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

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