Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3

  • 类型:
  • 作者:Yu Leiye, Sun Guojie, Wei Jingfang, Wang Yingze, Du Chao, Li Jiang
  • 期刊:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
  • 阅读原文

An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF)α secretion, and phagocytic ability. Furthermore, TAK-242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and IκBα degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-κB signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response.

文章引用产品列表