T细胞活化与极化检测系列:T细胞活化

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http://www.liankebio.com/article-information_Newsletter-417.html

作者:联科生物 发布日期:2016-02-16 00:00

T细胞要达到完全活化,至少需要两个信号:第一信号是由TCR识别抗原-主要组织相容性抗原MHC所启动;第二信号是由T细胞与协同共刺激分子相互作用所启动。而T细胞最重要的共刺激分子是CD28. 这些信号被传送到细胞核内后,会引起:

1.T细胞的克隆性增殖;

2.细胞表面活化标记物的上调

3.效应细胞的分化

4.细胞毒性诱导或细胞因子分泌

5.凋亡诱导反应
T细胞活化示意图
T细胞活化示意图

体外T细胞活化方案步骤:
eBio:http://media.ebioscience.com/data/pdf/best-protocols/t-cell-activation-in-vitro.pdf

产品列表:

Name Clone Application Cat#
Anti-Mouse CD3e Functional Grade Purified 50 ug 145-2C11 FC, FA 85-16-0031-81
Anti-Mouse CD3e Functional Grade Purified 100 ug 85-16-0031-82
Anti-Mouse CD3e Functional Grade Purified 500 ug 85-16-0031-85
Anti-Mouse CD3e Functional Grade Purified 1 mg 85-16-0031-86
Anti-Mouse CD28 Functional Grade Purified 50 ug 37.51 FC, FA 85-16-0281-81
Anti-Mouse CD28 Functional Grade Purified 100 ug 85-16-0281-82
Anti-Mouse CD28 Functional Grade Purified 500 ug 85-16-0281-85
Anti-Mouse CD28 Functional Grade Purified 1 mg 85-16-0281-86
Anti-Human CD3 Functional Grade Purified 50 ug OKT3 FC, FA 85-16-0037-81
Anti-Human CD3 Functional Grade Purified 500 ug 85-16-0037-85
Anti-Human CD3 Functional Grade Purified 50 ug HIT3a FC, FA 85-16-0039-81
Anti-Human CD3 Functional Grade Purified 500 ug 85-16-0039-85
Anti-Human CD28 Functional Grade Purified 50 ug CD28.2 FC, FA 85-16-0289-81
Anti-Human CD28 Functional Grade Purified 500 ug 85-16-0289-85