http://www.liankebio.com/article-information_Newsletter-1299.html
作者:联科生物 发布日期:2013-09-04 09:00
您还在使用Sepharose/琼脂糖悬浮液吗?
数以千计的科学家已经不再使用Sepharose®和琼脂糖。 他们现在更喜欢使用磁珠。
【 图 1: Dynabeads®磁珠分离是增长速度最快的免疫沉淀方法。 图表显示了使用琼脂糖 (绿色)、sepharose (蓝色) 和Dynabeads® (红色) 发表的论文增长/下降百分比。 来源: Google Scholar, Feb. 2011.】
【图 2: Dynabeads® Protein A磁珠与其他厂家的产品比较,可获得高纯度高回收率的蛋白。】
免疫沉淀最关键的是什么?
无背景:最低的非特异性结合 |
信噪比:简单高效的冲洗 |
操作简单:磁珠分离实验方案 |
节约抗体:结合于外表面性 |
时间和精力:30分钟完成 |
重复性:产品和操作一致性 |
Dynabeads® 让您的研究工具实现现代化!
简单、快速且高效结合 |
不会损失靶蛋白 |
无需柱子或离心 |
最高的灵敏度 |
无需将样本进行前处理(包括尿液、血清、血浆、腹水、组织培养物或杂交瘤细胞上清) |
【表1 – Dynabeads® Protein A/G的亲和程度蛋白。】
Ig来源 |
Protein A |
Protein G |
Human IgG1,2,4 |
Strong |
Strong |
Human IgG3 |
No binding |
Strong |
Human IgA, D, E |
Weak |
No binding |
Mouse IgG1 |
Weak |
Strong |
Mouse IgG2a, 2b, 3 |
Strong |
Strong |
Mouse IgM |
Weak |
No binding |
Rat IgG1 |
Weak |
Weak |
Rat IgG2a |
No binding |
Strong |
Rat IgG2b |
No binding |
Weak |
Rat IgG2c |
Strong |
Strong |
Bovine IgG1 |
Weak |
Strong |
Bovine IgG2 |
Strong |
Strong |
Chicken IgY |
No binding |
No binding |
Dog IgG |
Strong |
Weak |
Goat IgG1 |
Weak |
Strong |
Goat IgG2 |
Strong |
Strong |
Guinea pig IgG |
Strong |
Weak |
Hamster |
Weak |
- |
Horse IgG |
Weak |
Strong |
Monkey IgG |
Strong |
Strong |
Porcine IgG |
Strong |
Strong |
Rabbit IgG |
Strong |
Strong |
Sheep IgG1 |
Weak |
Strong |
Sheep IgG2 |
Strong |
Strong |
【相关产品:】
产品名称 |
货号 |
规格 |
应用 |
目录价(¥) |
Immunoprecipitation Kit - Dynabeads® Protein A |
10006D |
2ml |
IP, ChIP, Others |
3884.00 |
Immunoprecipitation Kit - Dynabeads® Protein G |
10007D |
2ml |
IP, ChIP, Others |
3884.00 |
Dynabeads® Protein A |
10001D |
1ml |
IP, ChIP, Others |
1630.00 |
Dynabeads® Protein A |
10002D |
5ml |
IP, ChIP, Others |
6792.00 |
Dynabeads® Protein A |
10008D |
50ml |
IP, ChIP, Others |
51000.00 |
Dynabeads® Protein G |
10003D |
1ml |
IP, ChIP, Others |
1630.00 |
Dynabeads® Protein G |
10004D |
5ml |
IP, ChIP, Others |
6792.00 |
Dynabeads® Protein G |
10009D |
50ml |
IP, ChIP, Others |
51000.00 |
Dynabeads® Antibody Coupling Kit |
14311D |
1kit |
|
4647.00 |
Dynabeads® Co- Immunoprecipitation Kit |
14321D |
1kit |
|
7779.00 |
|