P2107

Recombinant Mouse LIF

¥700.00 – ¥3,300.00

说明书

分子靶点：LIF, CDF, DIA
种属：小鼠 (Mouse)
表达宿主：Escherichia coli
纯度：≥95% as determined by SDS-PAGE
内毒素：<1EU/ug

在售SKU: P2107, 70-P2107-10, 70-P2107-100, 70-P2107-50

描述

文章目录[隐藏]

General Information
Specifications
Data
分子信息
LIF 分子靶点信息概述
LIF 分子靶点综述
- 小鼠 Mouse Lif 分子靶点信息
- 小鼠 Mouse Lif 靶点分子功能（预测）

General Information

Source	Escherichia coli
Synonyms	Leukemia inhibitory factor; CDF; DIA; HILDA
Accession	P09056
Molecular Mass	19.9 KDa
Description	Recombinant Mouse LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.
Shipping	The product is shipped at 4 ℃ . Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Aliquot the reconstituted solution to minimize freeze-thaw cycles. Lyophilized protein should be stored at -20℃ to -80℃, stable for one year after receipt. Aliquots of reconstituted samples are stable at ≤ -20℃ for 3 months.
Reconstitution	Centrifuge tubes before opening. Dissolve the lyophilized protein in distilled water. Do not mix by vortex. It is recommended to dissolve the product at a concentration of twice the specified specification.
Background	Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.

Specifications

Formulation	Lyophilized from sterile PBS, pH 7.4, 5% trehalose.
Purity	≥95% as determined by SDS-PAGE
Endotoxin	< 1EU/ug
Bio Activity	Immobilized Anti-Mouse LIF Antibody at 0.2 ug/mL (50 uL/well) can bind Mouse LIF with a linear range of 48.83 - 6,250 pg/mL.

Data

Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity — Immobilized Anti-Mouse LIF Antibody at 0.2 ug/mL (50 uL/well) can bind Mouse LIF with a linear range of 48.83 - 6,250 pg/mL. (EC50 = 0.76 ng/mL)

分子信息

概述小鼠 LIF, CDF, DIA 靶点信息

LIF 分子靶点信息概述

分子名：LIF, LIF interleukin 6 family cytokine
基因家族：Receptor ligands; Interleukin 6 type cytokine family
别名：CDF; DIA; HILDA
全称：differentiation inhibitory activity; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; human interleukin in DA cells; leukemia inhibitory factor; LIF, interleukin 6 family cytokine

LIF 分子靶点综述

白血病抑制因子(LIF)是一种白细胞介素6家族细胞因子，通过抑制分化影响细胞生长。当LIF水平下降时，细胞就会分化。活化的T细胞、单核细胞、星形胶质细胞、成骨细胞、角质形成细胞、肥大细胞和成纤维细胞都可表达LIF。LIF因其能诱导髓系白血病细胞的终端分化，阻止白血病细胞的持续增长而得名。LIF的其它特性包括促进生长、促进不同类型的靶细胞分化、影响骨代谢、恶病质、神经发育、胚胎发育和炎症。LIF参与孕期子宫内膜蜕膜化和胚胎植入子宫内膜的过程。女性体内的LIF和其它细胞因子水平下降时，就容易怀孕，但是不明原因复发性流产的风险会增加。研究表明重组人LIF可能有助于改善女性不明原因不孕不育的着床率。另外，LIF通常用来添加到干细胞培养基中来减少自发分化。

小鼠 Mouse Lif 分子靶点信息

分子名：Lif, leukemia inhibitory factor
别称：
- 无
基因序列：NCBI_Gene: 16878
蛋白序列：

小鼠 Mouse Lif 靶点分子功能（预测）

Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).