P133

Recombinant Human IL-33

¥700.00 – ¥3,300.00

说明书

分子靶点：IL33, IL-33, DVS27
种属：人 (Human)
表达宿主：Escherichia coli
纯度：≥95% as determined by SDS-PAGE
内毒素：<1EU/ug

在售SKU: P133, 70-P133-10, 70-P133-100, 70-P133-50

描述

文章目录[隐藏]

General Information
Specifications
Data
分子信息
IL33 分子靶点信息概述
IL33 分子靶点综述
- 人 Human IL33 分子靶点信息
- 人 Human IL33靶点分子功能（预测）

General Information

Source	Escherichia coli
Synonyms	Interleukin-33, C9orf26, DVS27, IL1F11, NFHEV
Accession	O95760
Molecular Mass	17.9 kDa
Description	Recombinant Human IL-33 is a 17.9 kDa protein containing 159 amino acid residues.
Shipping	The product is shipped at 4 ℃ . Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Aliquot the reconstituted solution to minimize freeze-thaw cycles. Lyophilized protein should be stored at -20℃ to -80℃, stable for one year after receipt. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Reconstitution	Centrifuge tubes before opening. Dissolve the lyophilized protein in distilled water. Do not mix by vortex. It is recommended to dissolve the product at a concentration of twice the specified specification.
Background	IL-33 is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.

Specifications

Formulation	Lyophilized from sterile PBS, pH 7.4, 5% trehalose.
Purity	≥95% as determined by SDS-PAGE
Endotoxin	< 1EU/ug
Bio Activity	Immobilized Anti-Human IL-33 Antibody at 0.2 ug/mL (50 uL/well) can bind Human IL-33 with a linear range of 1.56 - 200 ng/mL.

Data

Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity — Recombinant Human EGF can be detected by ELISA with a linear range of 2.0 - 2,500.0pg/mL. (EC50 = 95.18 pg/mL)

分子信息

概述人 Human

IL33 分子靶点信息概述

分子名：IL33, interleukin 33
基因家族：Interleukins
别名：DVS27; DKFZp586H0523; NF-HEV; IL1F11
曾用名：C9orf26
全称：DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)

IL33 分子靶点综述

白细胞介素33(IL-33)也称为NF-HEV和DVS27，是IL-1家族成员，分子量为30kDa，由多种类型的细胞分泌，包括成纤维细胞、肥大细胞、树突细胞、巨噬细胞、成骨细胞、内皮细胞和上皮细胞。IL-33可诱导辅助性T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞分泌II型细胞因子。IL-33通过与受体ST2和IL-1受体辅助蛋白(IL1RAP)结合，活化可促使极化的Th2细胞分泌II型细胞因子(如IL-5和IL-13)的NF-κB和MAP激酶信号通路中的胞外分子来介导其生物学功能。注射IL-33后，其可在体内诱导II型细胞因子，这被认为可诱导粘膜器官发生严重的病理变化。

人 Human IL33 分子靶点信息

分子名：IL33, interleukin 33
别称：
- C9orf26
- DKFZp586H0523
- DVS27
- DVS27-related protein
- IL-1F11
- IL-33
- IL1F11
- interleukin-1 family member 11
- interleukin-33
- NF-HEV
- NFEHEV
- nuclear factor for high endothelial venules
- nuclear factor from high endothelial venules
- RP11-575C20.2
基因序列：NCBI_Gene: 90865
蛋白序列：UniProtKB: O95760

人 Human IL33靶点分子功能（预测）

Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).