EK383HS

Rat VEGF High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：VEGFA, VEGF, VPF
  • 种属：大鼠 (Rat)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.34 pg/mL
  • 检测范围：3.13 pg/mL - 200 pg/mL
  • 回收率：91% - 111%

在售SKU:70-EK383HS-48, 70-EK383HS-96

描述

商品名

Rat VEGF High Sensitivity ELISA Kit (大鼠血管内皮生长因子高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.2% - 4.8%；板间变异系数：4.6% - 5.0%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.34 pg/mL
检测范围

3.13 pg/mL - 200 pg/mL
回收率

91% - 111%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗VEGFA单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠VEGFA冻干标准品
  • VEGFA检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的VEGF与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat VEGF High Sensitivity ELISA Kit (大鼠血管内皮生长因子高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

  • 分子名：VEGFA, vascular endothelial growth factor A
  • 基因家族：VEGF family
  • 别名：VEGF-A; VPF
  • 曾用名：VEGF
  • 全称：vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。

大鼠 Rat Vegfa 分子靶点信息

大鼠 Rat Vegfa 靶点分子功能（预测）

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Chronic stress-induced depression requires the recruitment of peripheral Th17 cells into the brain 

  2. Use of bioactive extracellular matrix fragments as a urethral bulking agent to treat stress urinary incontinence 

  3. Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes 

  4. Guanxinning tablet inhibits the interaction between leukocyte integrin Mac-1 and platelet GPIbα for antithrombosis without increased bleeding risk 

  5. Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study 

  6. Extracellular matrix sheet modified with VEGF-loaded nanoparticles for bladder regeneration 

  7. Chitosan-Gelatin Scaffolds Incorporating Decellularized Platelet-Rich Fibrin Promote Bone Regeneration 

  8. Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis 

  9. Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats 

  10. Vascular endothelial growth factor-loaded poly-lactic-co-glycolic acid nanoparticles with controlled release protect the dopaminergic neurons in Parkinson's rats 

  11. Qiliqiangxin attenuates hypoxia-induced injury in primary rat cardiac microvascular endothelial cells via promoting HIF-1α-dependent glycolysis 

  12. Catalpol Promotes the Survival and VEGF Secretion of Bone Marrow-Derived Stem Cells and Their Role in Myocardial Repair After Myocardial Infarction in Rats 

  13. Correlation of Vascular Endothelial Growth Factor Production with Photochemical Reaction-induced Retinal Edema 

  14. Transcutaneous Auricular Vagus Nerve Stimulation Promotes White Matter Repair and Improves Dysphagia Symptoms in Cerebral Ischemia Model Rats 

  15. Immunomodulation of Telmisartan-Loaded PCL/PVP Scaffolds on Macrophages Promotes Endogenous Bone Regeneration 

  16. Antibacterial sequential growth factor delivery from alginate/gelatin methacryloyl microspheres for bone regeneration 

  17. ROS-scavenging lipid-based liquid crystalline as a favorable stem cell extracellular vesicles delivery vector to promote wound healing 

  18. Oxygen tension regulating hydrogels for vascularization and osteogenesis via sequential activation of HIF-1α and ERK1/2 signaling pathways in bone regeneration 

  19. Spatiotemporal modulation of immune microenvironment via composite hydrogel brakes for diabetic wound healing 

  20. CARMA3 Drives NF-κB Activation and Promotes Intervertebral Disc Degeneration: Involvement of CARMA3-BCL10-MALT1 Signalosome 

  21. Zinc-loaded human serum albumin methacryloyl microspheres with growth factors release for infected wound healing 

  22. Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages 

  23. An anti-fibroblast adhesion and anti-inflammatory hydrogel film combined with VEGF for intrauterine adhesion prevention 

  24. Resveratrol-induced SIRT1 activation inhibits glycolysis-fueled angiogenesis under rheumatoid arthritis conditions independent of HIF-1α 

  25. Insulin modified Decellularized Adipose Tissue/Tremella Polysaccharide hydrogel loaded with ADSCs for skin wound healing 

  26. ROS filter coating scaffold protects 3D mesenchymal stem cell spheroids for dual-phase treatment of spinal cord injury 

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