EK383

Rat VEGF ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：VEGFA, VEGF, VPF
  • 种属：大鼠 (Rat)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：3.86 pg/mL
  • 检测范围：39.06 pg/mL - 2500 pg/mL
  • 回收率：83% - 114%

在售SKU:70-EK383/2-48, 70-EK383/2-96

描述

商品名

Rat VEGF ELISA Kit (大鼠血管内皮生长因子 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.4% - 6.3%；板间变异系数：4.4% - 6.0%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

3.86 pg/mL
检测范围

39.06 pg/mL - 2500 pg/mL
回收率

83% - 114%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗VEGFA单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠VEGFA冻干标准品
  • VEGFA检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的VEGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat VEGF ELISA Kit (大鼠血管内皮生长因子 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

  • 分子名：VEGFA, vascular endothelial growth factor A
  • 基因家族：VEGF family
  • 别名：VEGF-A; VPF
  • 曾用名：VEGF
  • 全称：vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。

大鼠 Rat Vegfa 分子靶点信息

大鼠 Rat Vegfa 靶点分子功能（预测）

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Use of bioactive extracellular matrix fragments as a urethral bulking agent to treat stress urinary incontinence 

  2. In vitro study of decellularized rat tissues for nerve regeneration. 

  3. Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes 

  4. Guanxinning tablet inhibits the interaction between leukocyte integrin Mac-1 and platelet GPIbα for antithrombosis without increased bleeding risk 

  5. Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study 

  6. Extracellular matrix sheet modified with VEGF-loaded nanoparticles for bladder regeneration 

  7. Chitosan-Gelatin Scaffolds Incorporating Decellularized Platelet-Rich Fibrin Promote Bone Regeneration 

  8. Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis 

  9. Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats 

  10. Vascular endothelial growth factor-loaded poly-lactic-co-glycolic acid nanoparticles with controlled release protect the dopaminergic neurons in Parkinson's rats 

  11. Qiliqiangxin attenuates hypoxia-induced injury in primary rat cardiac microvascular endothelial cells via promoting HIF-1α-dependent glycolysis 

  12. Catalpol Promotes the Survival and VEGF Secretion of Bone Marrow-Derived Stem Cells and Their Role in Myocardial Repair After Myocardial Infarction in Rats 

  13. Correlation of Vascular Endothelial Growth Factor Production with Photochemical Reaction-induced Retinal Edema 

  14. MiR-185-5p Protects Against Angiogenesis in Polycystic Ovary Syndrome by Targeting VEGFA 

  15. Transcutaneous Auricular Vagus Nerve Stimulation Promotes White Matter Repair and Improves Dysphagia Symptoms in Cerebral Ischemia Model Rats 

  16. Immunomodulation of Telmisartan-Loaded PCL/PVP Scaffolds on Macrophages Promotes Endogenous Bone Regeneration 

  17. Antibacterial sequential growth factor delivery from alginate/gelatin methacryloyl microspheres for bone regeneration 

  18. PLK-3-mediated phosphorylation of BAP1 prevents diabetic retinopathy 

  19. ROS-scavenging lipid-based liquid crystalline as a favorable stem cell extracellular vesicles delivery vector to promote wound healing 

  20. Oxygen tension regulating hydrogels for vascularization and osteogenesis via sequential activation of HIF-1α and ERK1/2 signaling pathways in bone regeneration 

  21. Spatiotemporal modulation of immune microenvironment via composite hydrogel brakes for diabetic wound healing 

  22. CARMA3 Drives NF-κB Activation and Promotes Intervertebral Disc Degeneration: Involvement of CARMA3-BCL10-MALT1 Signalosome 

  23. Zinc-loaded human serum albumin methacryloyl microspheres with growth factors release for infected wound healing 

  24. An injectable, activated neutrophil-derived exosome mimetics/extracellular matrix hybrid hydrogel with antibacterial activity and wound healing promotion effect for diabetic wound therapy 

  25. Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages 

  26. An anti-fibroblast adhesion and anti-inflammatory hydrogel film combined with VEGF for intrauterine adhesion prevention 

  27. Resveratrol-induced SIRT1 activation inhibits glycolysis-fueled angiogenesis under rheumatoid arthritis conditions independent of HIF-1α 

  28. Loss of Histone Methyltransferase KMT2D Attenuates Angiogenesis in the Ischemic Heart by Inhibiting the Transcriptional Activation of VEGF-A 

  29. Insulin modified Decellularized Adipose Tissue/Tremella Polysaccharide hydrogel loaded with ADSCs for skin wound healing 

  30. ROS filter coating scaffold protects 3D mesenchymal stem cell spheroids for dual-phase treatment of spinal cord injury 

  31. 细胞培养上清

    Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis 

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