Rat sICAM-1/CD54 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:ICAM1, BB2, CD54
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:0.48 pg/mL
- 检测范围:62.5 pg/mL - 4000 pg/mL
- 回收率:73% - 90%
在售SKU:70-EK389-48, 70-EK389-96
描述
商品名 |
Rat sICAM-1/CD54 ELISA Kit (大鼠细胞间粘附分子1 (ICAM-1) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.8% - 4.2%;板间变异系数:2.7% - 3.4% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
0.48 pg/mL |
检测范围 |
62.5 pg/mL - 4000 pg/mL |
回收率 |
73% - 90% |
平均回收率 |
0.83 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠sICAM-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sICAM-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sICAM-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
ICAM1 分子靶点信息概述
- 分子名:ICAM1, intercellular adhesion molecule 1
- 基因家族:CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
- 别名:BB2; CD54
- 全称:human rhinovirus receptor
ICAM1 分子靶点综述
细胞间粘附分子1(ICAM-1)也称为CD54,是一个几乎无所不在的跨膜糖蛋白,通常表达于内皮细胞和免疫细胞,对白细胞迁移和活化具有关键作用。它与CD11a/CD18或CD11b/CD18型整合素结合,也会被鼻病毒作为受体利用。可溶性细胞间粘附分子1(sICAM-1)促进血管生成,可作为血管内皮细胞活化或受损的指示剂。它也可作为跨膜ICAM-1抑制剂,介导活性如单核细胞对活化的内皮细胞的黏附以及肿瘤细胞对NK细胞介导的溶解作用的敏感性。监测血清中的sICAM-1表达水平可对多种病理状态提供更加详细的视角,如心血管疾病、II型糖尿病、器官移植障碍、氧化应激、腹部脂肪团、高血压、肝脏疾病和某些恶性肿瘤等。
大鼠 Rat Icam1 分子靶点信息
- 分子名:Icam1, intercellular adhesion molecule 1
- 别称:
- CD54
- ICAM
- ICAM-1
- 基因序列:NCBI_Gene: 25464
- 蛋白序列:UniProtKB: Q00238
大鼠 Rat Icam1 靶点分子功能(预测)
Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Bone marrow mesenchymal stem cells combine with normothermic machine perfusion to improve rat donor liver quality—the important role of hepatic microcirculation in donation after circulatory death 
- The dynamic changes in autophagy activity and its role in lung injury after deep hypothermic circulatory arrest 
- Pterostilbene Attenuates Subarachnoid Hemorrhage-Induced Brain Injury through the SIRT1-Dependent Nrf2 Signaling Pathway 
- SIRT1 Promotes M2 Microglia Polarization via Reducing ROS-Mediated NLRP3 Inflammasome Signaling After Subarachnoid Hemorrhage 
- Electroacupuncture treatment improves postoperative ileus by inhibiting the Th1 cell-mediated inflammatory response through the vagus nerve 
Bone marrow mesenchymal stem cells combine with normothermic machine perfusion to improve rat donor liver quality—the important role of hepatic microcirculation in donation after circulatory death 
The dynamic changes in autophagy activity and its role in lung injury after deep hypothermic circulatory arrest 
Pterostilbene Attenuates Subarachnoid Hemorrhage-Induced Brain Injury through the SIRT1-Dependent Nrf2 Signaling Pathway 
SIRT1 Promotes M2 Microglia Polarization via Reducing ROS-Mediated NLRP3 Inflammasome Signaling After Subarachnoid Hemorrhage 
Electroacupuncture treatment improves postoperative ileus by inhibiting the Th1 cell-mediated inflammatory response through the vagus nerve 
评价
目前还没有评价