Rat Leptin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,200.00 – ¥3,700.00
在售SKU:70-EK397-48, 70-EK397-96, EK397, 大鼠瘦素 大鼠 瘦素
- 分子靶点:LEP
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:1.14pg/mL
- 检测范围:62.5-4,000pg/mL
- 回收率:92%-130%
ELISA试剂盒详细信息
商品名 | Rat Leptin 酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | LEP |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 1.14pg/mL |
线性范围 | 62.5-4,000pg/mL |
精密度 | 板内变异系数:3.5%-4.6%;板间变异系数:4.2%-4.7% |
回收率 | 92%-130% |
平均回收率 | 107% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠Leptin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Leptin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Leptin的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
LEP 分子靶点信息概述
- 分子名:LEP, leptin
- 基因家族:Neuropeptides
- 曾用名:OBS; OB
- 全称:leptin (murine obesity homolog); leptin (obesity homolog, mouse)
LEP 分子靶点综述
瘦素是由脂肪细胞分泌的蛋白质类激素,可通过抑制饥饿来调控能量平衡。除了白色脂肪组织可以分泌瘦素外,褐色脂肪、胎盘、卵巢、骨骼肌、胃、乳腺上皮细胞、骨髓和P/D1细胞也能产生瘦素。瘦素和饥饿激素都作用于下丘脑弓状核的受体,以此来调节食欲,获得能量平衡。瘦素的基本功能是调节脂肪储存,但它在其它生理代谢过程中同样发挥作用,然而许多其它功能还未明确。研究表明瘦素与肥胖和减肥有关联。
大鼠 Rat Lep 分子靶点信息
- 分子名:Lep, leptin
- 别称:
- OB
- obese
- obesity (murine homolog leptin)
- obesity factor
- 基因序列:NCBI_Gene: 25608
- 蛋白序列:
大鼠 Rat Lep 靶点分子功能(预测)
Enables hormone activity and peptide hormone receptor binding activity. Involved in several processes, including positive regulation of cell communication; regulation of apoptotic process; and response to vitamin. Located in cytosol and extracellular space. Used to study several diseases, including alcohol dependence; glucose metabolism disease (multiple); liver disease (multiple); neurodegenerative disease (multiple); and short bowel syndrome. Biomarker of several diseases, including Huntington's disease; alcohol dependence; fatty liver disease (multiple); lipid metabolism disorder (multiple); and obesity. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human LEP (leptin).
引用文献统计
该产品被引用的文献总数为:5
- The Mechanism of Zinc Sulfate in Improving Fertility in Obese Rats Analyzed by Sperm Proteomic Analysis
影响因子:2.276刊物:Biomed Research International发表日期:2020/5/5 - Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats
影响因子:6.706刊物:Nutrients发表日期:2022/9/2 - Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling
影响因子:4.162刊物:Drug Design Development and Therapy发表日期:2021/7/16 - Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota
影响因子:4刊物:Frontiers in Nutrition发表日期:2024-08-14 - White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy
影响因子:6.8刊物:BRITISH JOURNAL OF PHARMACOLOGY发表日期:2024-04-21