EK387

Rat JE/MCP-1/CCL2 ELISA Kit检测试剂盒（酶联免疫吸附法）

X

¥2,000.00 – ¥3,400.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：CCL2, MCP-1, MCAF, SMC-CF
  • 种属：大鼠 (Rat)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：100 μL(稀释液)
  • 灵敏度：0.50 pg/mL
  • 检测范围：7.81 pg/mL - 500 pg/mL
  • 回收率：84% - 110%

在售SKU:70-EK387-48, 70-EK387-96

描述

商品名

Rat JE/MCP-1/CCL2 ELISA Kit (大鼠 JE/MCP-1/CCL2 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.3% - 5.0%；板间变异系数：4.3%-9.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

100 μL(稀释液)
灵敏度

0.50 pg/mL
检测范围

7.81 pg/mL - 500 pg/mL
回收率

84% - 110%
平均回收率

1.03
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗大鼠CCL2单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠CCL2冻干标准品
  • 生物素标记的大鼠CCL2检测抗体
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的CCL2与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat JE/MCP-1/CCL2 ELISA Kit (大鼠 JE/MCP-1/CCL2 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 CCL2, MCP-1, MCAF, SMC-CF 靶点信息

CCL2 分子靶点信息概述

  • 分子名：CCL2, C-C motif chemokine ligand 2
  • 基因家族：Chemokine ligands
  • 别名：MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • 曾用名：SCYA2
  • 全称：monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2 分子靶点综述

趋化因子配体2(CCL2)，又名单核细胞趋化蛋白1(MCP-1)，是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上，主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制，如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程，表现为神经元变性。

大鼠 Rat Ccl2 分子靶点信息

  • 分子名：Ccl2, C-C motif chemokine ligand 2
  • 别称：
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • immediate-early serum-responsive JE protein
    • immediate-early serum-responsive protein JE
    • MCP-1
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic protein 1
    • Scya2
    • Sigje
    • small inducible cytokine A2
    • Small inducible gene JE
    • small-inducible cytokine A2
  • 基因序列：NCBI_Gene: 24770
  • 蛋白序列：UniProtKB: P14844

大鼠 Rat Ccl2 靶点分子功能（预测）

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and leukocyte migration. Located in several cellular components, including perikaryon; perinuclear region of cytoplasm; and rough endoplasmic reticulum. Colocalizes with C-fiber. Used to study several diseases, including artery disease (multiple); diabetic angiopathy; interstitial lung disease (multiple); kidney disease (multiple); and pancreatitis (multiple). Biomarker of several diseases, including artery disease (multiple); cerebrovascular disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling 

  2. Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 

  3. Analysis of the Potential Relationship between Aging and Pulmonary Fibrosis Based on Transcriptome 

  4. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro 

  5. α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

  6. Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling 

  7. Gut derived-endotoxin contributes to inflammation in severe ischemic acute kidney injury 

  8. Stellate Ganglion Block Combined with Dexmedetomidine Protects Obese Rats from Lipopolysaccharide-Induced Acute Lung Injury 

  9. PTPRO knockdown protects against inflammation in hemorrhage shock-induced lung injury involving the NF-κB signaling pathway 

  10. SIRT1 Promotes M2 Microglia Polarization via Reducing ROS-Mediated NLRP3 Inflammasome Signaling After Subarachnoid Hemorrhage 

  11. An Shen Ding Zhi Ling Alleviates Symptoms of Attention Deficit Hyperactivity Disorder via Anti-Inflammatory Effects in Spontaneous Hypertensive Rats 

  12. The Anti-Inflammatory Effect of Feiyangchangweiyan Capsule and Its Main Components on Pelvic Inflammatory Disease in Rats via the Regulation of the NF-κB and BAX/BCL-2 Pathway 

  13. SHP-1 suppresses endotoxin-induced uveitis by inhibiting the TAK1/JNK pathway 

  14. Salidroside prevents tumor necrosis factor‑α‑induced vascular inflammation by blocking mitogen‑activated protein kinase and NF‑κB signaling activation 

  15. A highly selective inhibitor of discoidin domain receptor-1 (DDR1-IN-1) protects corneal epithelial cells from YAP/ACSL4-mediated ferroptosis in dry eye 

  16. Epithelial cells derived exosomal miR-203a-3p facilitates stromal inflammation of type IIIA chronic prostatitis/chronic pelvic pain syndrome by targeting DUSP5 and increasing MCP-1 generation 

  17. White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy 

  18. The Effect of an Er:YAG Laser Combined with a 755?nm Picosecond Laser on Tattoo Removal in a Rat Model 

  19. Vitamin D Improves Klebsiella-Induced Severe Pneumonia in Rats by Regulating Intestinal Microbiota 

  20. HMGA2 knockdown alleviates the progression of nonalcoholic fatty liver disease (NAFLD) by downregulating SNAI2 expression 

  21. Multifunctional chondroitin sulfate based hydrogels for promoting infected diabetic wounds healing by chemo-photothermal antibacterial and cytokine modulation 

  22. Myricetin ameliorates experimental autoimmune myocarditis in mice by modulating immune response and inhibiting MCP-1 expression 

  23. A highly selective inhibitor of discoidin domain receptor-1 (DDR1-IN-1) protects corneal epithelial cells from YAP/ACSL4-mediated ferroptosis in dry eye 

  24. A highly selective inhibitor of discoidin domain receptor-1 (DDR1-IN-1) protects corneal epithelial cells from YAP/ACSL4-mediated ferroptosis in dry eye 

  25. 血清

    Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

评价

目前还没有评价

成为第一个“Rat JE/MCP-1/CCL2 ELISA Kit检测试剂盒（酶联免疫吸附法）” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注

相关产品