EK306HS

Rat IL-6 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL6, IL-6, IFNB2, HGF
  • 种属：大鼠 (Rat)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：1.8 pg/mL
  • 检测范围：7.81 pg/mL - 500 pg/mL
  • 回收率：95% - 106%

在售SKU:70-EK306HS-48, 70-EK306HS-96

描述

商品名

Rat IL-6 High Sensitivity ELISA Kit (大鼠白细胞介素6 高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：6.4% - 9.1%；板间变异系数：6.1% - 9.6%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

1.8 pg/mL
检测范围

7.81 pg/mL - 500 pg/mL
回收率

95% - 106%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-6冻干标准品
  • IL-6检测抗体
  • 样本稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-6与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-6 High Sensitivity ELISA Kit (大鼠白细胞介素6 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名：IL6, interleukin 6
  • 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名：IL-6; BSF2; HGF; HSF
  • 曾用名：IFNB2
  • 全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

大鼠 Rat Il6 分子靶点信息

  • 分子名：Il6, interleukin 6
  • 别称：
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • 基因序列：NCBI_Gene: 24498
  • 蛋白序列：UniProtKB: P20607

大鼠 Rat Il6 靶点分子功能（预测）

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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