Rat IL-17A High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
- 分子靶点:IL17A, IL17, CTLA8
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.15 pg/mL
- 检测范围:1.56 pg/mL - 100 pg/mL
- 回收率:94% - 117%
在售SKU:70-EK317HS-48, 70-EK317HS-96
描述
商品名 |
Rat IL-17A High Sensitivity ELISA Kit (大鼠白细胞介素17A高敏 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.5% - 4.5%;板间变异系数:2.9% - 4.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.15 pg/mL |
检测范围 |
1.56 pg/mL - 100 pg/mL |
回收率 |
94% - 117% |
平均回收率 |
1.02 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-17A与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL17A 分子靶点信息概述
- 分子名:IL17A, interleukin 17A
- 基因家族:Interleukins
- 别名:IL-17A; IL-17
- 曾用名:CTLA8; IL17
- 全称:cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
IL17A 分子靶点综述
IL-17主要由Th17型细胞产生,是目前已发现的30余种白细胞介素中的一员,按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子,可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子,从而导致炎症的产生。
大鼠 Rat Il17a 分子靶点信息
- 分子名:Il17a, interleukin 17A
- 别称:
- CTLA-8
- cytotoxic T-lymphocyte-associated antigen 8
- hypothetical protein LOC301289
- IL-17
- IL-17A
- Il17
- interleukin 17
- Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
- interleukin-17A
- LOC301289
- similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
- 基因序列:NCBI_Gene: 301289
- 蛋白序列:
大鼠 Rat Il17a 靶点分子功能(预测)
Predicted to enable protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including cellular response to glucocorticoid stimulus; positive regulation of necrotic cell death; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and extracellular space. Used to study anti-basement membrane glomerulonephritis; congestive heart failure; myocarditis; pleurisy; and uveitis. Biomarker of several diseases, including acute necrotizing pancreatitis; inflammatory bowel disease (multiple); lipoid nephrosis; lung disease (multiple); and periodontal disease (multiple). Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Chronic stress-induced depression requires the recruitment of peripheral Th17 cells into the brain 
- Gut Microbiota Is Involved in Alcohol-Induced Osteoporosis in Young and Old Rats Through Immune Regulation 
- Bortezomib alleviates antibody-mediated rejection in kidney transplantation by facilitating Atg5 expression 
- Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury 
- Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway 
- Securidaca inappendiculata-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR-γ Signaling 
- Discovery and In Vivo Anti-inflammatory Activity Evaluation of a Novel Non-peptidyl Non-covalent Cathepsin C Inhibitor 
- IL-17 crosses the blood–brain barrier to trigger neuroinflammation: a novel mechanism in nitroglycerin-induced chronic migraine 
- Physicochemical Characteristics and Antidiabetic Properties of the Polysaccharides from Pseudostellaria heterophylla 
- Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance 
- Traditional Chinese Medicine Aconiti Radix Cocta Improves Rheumatoid Arthritis via Suppressing COX-1 and COX-2 
- Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance 
- Ethyl acetate extract of Tibetan medicine Rhamnella gilgitica ameliorated type II collagen-induced arthritis in rats via regulating JAK-STAT signaling pathway 
- Xanthones from Securidaca inappendiculata antagonized the antirheumatic effects of methotrexate in vivo by promoting its secretion into urine. 
- Dihydromyricetin Inhibits Inflammation of Fibroblast-Like Synoviocytes through Regulation of Nuclear Factor-κB Signaling in Rats with Collagen-Induced Arthritis 
- Tou Nong San Attenuates Inflammation in TNBS-IBD Model by Inhibiting NF-κB Signaling Pathway 
- Metabolomics combined with network pharmacology to study the mechanism of Shentong Zhuyu decoction in the treatment of rheumatoid arthritis 
- Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis 
- Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 
- The anti-rheumatoid arthritic activity of Artemisia ordosica Krasch. (traditional Chinese/Mongolian medicine) extract in collagen-induced arthritis in rats 
- Si-Miao-Yong-An Decoction alleviates thromboangiitis obliterans by regulating miR-548j-5p/IL-17A signaling pathway 
- Multi-omics integrated analysis indicated that non-polysaccharides of Sijunzi decoction ameliorated spleen deficiency syndrome via regulating microbiota-gut-metabolites axis and exerted synergistic compatibility 
- White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy 
- Autoantigenic Peptide and Immunomodulator Codelivery System for Rheumatoid Arthritis Treatment by Reestablishing Immune Tolerance 
- Lilrb4 ameliorates ileal injury in rats with hemorrhagic shock and suppresses the activation of NF-κB signaling pathway 
- Clostridium butyricum Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats 
Chronic stress-induced depression requires the recruitment of peripheral Th17 cells into the brain 
Gut Microbiota Is Involved in Alcohol-Induced Osteoporosis in Young and Old Rats Through Immune Regulation 
Bortezomib alleviates antibody-mediated rejection in kidney transplantation by facilitating Atg5 expression 
Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury 
Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway 
Securidaca inappendiculata-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR-γ Signaling 
Discovery and In Vivo Anti-inflammatory Activity Evaluation of a Novel Non-peptidyl Non-covalent Cathepsin C Inhibitor 
IL-17 crosses the blood–brain barrier to trigger neuroinflammation: a novel mechanism in nitroglycerin-induced chronic migraine 
Physicochemical Characteristics and Antidiabetic Properties of the Polysaccharides from Pseudostellaria heterophylla 
Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance 
Traditional Chinese Medicine Aconiti Radix Cocta Improves Rheumatoid Arthritis via Suppressing COX-1 and COX-2 
Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance 
Ethyl acetate extract of Tibetan medicine Rhamnella gilgitica ameliorated type II collagen-induced arthritis in rats via regulating JAK-STAT signaling pathway 
Xanthones from Securidaca inappendiculata antagonized the antirheumatic effects of methotrexate in vivo by promoting its secretion into urine. 
Dihydromyricetin Inhibits Inflammation of Fibroblast-Like Synoviocytes through Regulation of Nuclear Factor-κB Signaling in Rats with Collagen-Induced Arthritis 
Tou Nong San Attenuates Inflammation in TNBS-IBD Model by Inhibiting NF-κB Signaling Pathway 
Metabolomics combined with network pharmacology to study the mechanism of Shentong Zhuyu decoction in the treatment of rheumatoid arthritis 
Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis 
Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 
The anti-rheumatoid arthritic activity of Artemisia ordosica Krasch. (traditional Chinese/Mongolian medicine) extract in collagen-induced arthritis in rats 
Si-Miao-Yong-An Decoction alleviates thromboangiitis obliterans by regulating miR-548j-5p/IL-17A signaling pathway 
Multi-omics integrated analysis indicated that non-polysaccharides of Sijunzi decoction ameliorated spleen deficiency syndrome via regulating microbiota-gut-metabolites axis and exerted synergistic compatibility 
White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy 
Autoantigenic Peptide and Immunomodulator Codelivery System for Rheumatoid Arthritis Treatment by Reestablishing Immune Tolerance 
Lilrb4 ameliorates ileal injury in rats with hemorrhagic shock and suppresses the activation of NF-κB signaling pathway 
Clostridium butyricum Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats 
评价
目前还没有评价