Rat IL-17A ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL17A, IL17, CTLA8
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:0.62 pg/mL
- 检测范围:3.13 pg/mL - 200 pg/mL
- 回收率:94% - 119%
在售SKU:70-EK317/3-48, 70-EK317/3-96
描述
商品名 |
Rat IL-17A ELISA Kit (大鼠白细胞介素17A ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.9% - 7.1%;板间变异系数:3.0% - 3.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
0.62 pg/mL |
检测范围 |
3.13 pg/mL - 200 pg/mL |
回收率 |
94% - 119% |
平均回收率 |
1.03 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-17A与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL17A 分子靶点信息概述
- 分子名:IL17A, interleukin 17A
- 基因家族:Interleukins
- 别名:IL-17A; IL-17
- 曾用名:CTLA8; IL17
- 全称:cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
IL17A 分子靶点综述
IL-17主要由Th17型细胞产生,是目前已发现的30余种白细胞介素中的一员,按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子,可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子,从而导致炎症的产生。
大鼠 Rat Il17a 分子靶点信息
- 分子名:Il17a, interleukin 17A
- 别称:
- CTLA-8
- cytotoxic T-lymphocyte-associated antigen 8
- hypothetical protein LOC301289
- IL-17
- IL-17A
- Il17
- interleukin 17
- Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
- interleukin-17A
- LOC301289
- similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
- 基因序列:NCBI_Gene: 301289
- 蛋白序列:
大鼠 Rat Il17a 靶点分子功能(预测)
Predicted to enable protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including cellular response to glucocorticoid stimulus; positive regulation of necrotic cell death; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and extracellular space. Used to study anti-basement membrane glomerulonephritis; congestive heart failure; myocarditis; pleurisy; and uveitis. Biomarker of several diseases, including acute necrotizing pancreatitis; inflammatory bowel disease (multiple); lipoid nephrosis; lung disease (multiple); and periodontal disease (multiple). Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).
引用文献统计
该产品被引用的文献总数为:29
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