EK310HS

Rat IL-10 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL10, CSIF, TGIF
  • 种属：大鼠 (Rat)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：10 μL
  • 灵敏度：0.22 pg/mL
  • 检测范围：6.25 pg/mL - 400 pg/mL
  • 回收率：94% - 115%

在售SKU:70-EK310HS-48, 70-EK310HS-96

描述

商品名

Rat IL-10 High Sensitivity ELISA Kit (大鼠白细胞介素10 高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.4% - 4.8%；板间变异系数：3.9% - 4.9%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

10 μL
灵敏度

0.22 pg/mL
检测范围

6.25 pg/mL - 400 pg/mL
回收率

94% - 115%
平均回收率

1.03
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-10冻干标准品
  • IL-10检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-10与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-10 High Sensitivity ELISA Kit (大鼠白细胞介素10 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名：IL10, interleukin 10
  • 基因家族：Interleukins
  • 别名：CSIF; TGIF; IL10A; IL-10
  • 全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

大鼠 Rat Il10 分子靶点信息

大鼠 Rat Il10 靶点分子功能（预测）

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

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