Rat IL-18 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL18, IGIF, IL1F4
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:10 μL;细胞培养上清:100 μL
- 灵敏度:3.50 pg/mL
- 检测范围:15.63 pg/mL - 1000 pg/mL
- 回收率:86% - 119%
在售SKU:70-EK318-48, 70-EK318-96,EK318,il-18
描述
商品名 |
Rat IL-18 ELISA Kit (大鼠白细胞介素18 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.3% - 4.8%;板间变异系数:3.5% - 5.3% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
血清血浆:10 μL;细胞培养上清:100 μL |
灵敏度 |
3.50 pg/mL |
检测范围 |
15.63 pg/mL - 1000 pg/mL |
回收率 |
86% - 119% |
平均回收率 |
1.00 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物 (TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长 (参考波长 570 - 630 nm) 测定吸光度值。
分子信息
IL18 分子靶点信息概述
- 分子名:IL18, interleukin 18
- 基因家族:Interleukins
- 别名:IGIF; IL1F4; IL-1g; IL-18
- 全称:interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
IL18 分子靶点综述
白细胞介素18(IL-18),又名干扰素γ诱导因子,是一种促炎性细胞因子,在人类中由IL18基因编码。IL-18属于IL-1超家族,由巨噬细胞和其它细胞分泌产生。IL-18刺激后,自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素,进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应,同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。
大鼠 Mouse Il18 分子靶点信息
- 分子名:Il18, interleukin 18
- 别称:
- IFN-gamma-inducing factor
- IL-1 gamma
- IL-18
- interferon gamma-inducing factor
- interferon-gamma-inducing factor
- interleukin-1 gamma
- interleukin-18
- 基因序列:NCBI_Gene: 29197
- 蛋白序列:
大鼠 Rat Il18 靶点分子功能(预测)
Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
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- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway 
- Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
- Multi-omics integrated analysis indicated that non-polysaccharides of Sijunzi decoction ameliorated spleen deficiency syndrome via regulating microbiota-gut-metabolites axis and exerted synergistic compatibility 
- Mechanistic study of Huangqi Guizhi Wuwu decoction amelioration of doxorubicin-induced cardiotoxicity by reducing oxidative stress and inhibiting cellular pyroptosis 
- Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
- Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway 
Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
Multi-omics integrated analysis indicated that non-polysaccharides of Sijunzi decoction ameliorated spleen deficiency syndrome via regulating microbiota-gut-metabolites axis and exerted synergistic compatibility 
Mechanistic study of Huangqi Guizhi Wuwu decoction amelioration of doxorubicin-induced cardiotoxicity by reducing oxidative stress and inhibiting cellular pyroptosis 
Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
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