Rat IgA ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CD79A, IGA
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:0.48 ng/mL
- 检测范围:15.63 ng/mL - 1000 ng/mL
- 回收率:82% - 105%
在售SKU:70-EK374-48, 70-EK374-96
描述
商品名 |
Rat IgA ELISA Kit (大鼠免疫球蛋白A (IgA) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.7% - 7.9%;板间变异系数:3.9% - 8.6% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
0.48 ng/mL |
检测范围 |
15.63 ng/mL - 1000 ng/mL |
回收率 |
82% - 105% |
平均回收率 |
0.95 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IgA抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IgA与固相抗体结合。洗涤去除未结合物质后,加入辣根过氧化物酶标记的检测抗体孵育。洗涤去除未结合的检测抗体。加入显色底物TMB,避光显色。颜色反应的深浅与样本中IgA的浓度成正比。加入终止液终止反应,在450 nm波长 (参考波长570 - 630 nm)测定吸光度值。
分子信息
CD79A 分子靶点信息概述
- 分子名:CD79A, CD79a molecule
- 基因家族:CD molecules; V-set domain containing
- 别名:MB-1
- 曾用名:IGA
- 全称:B-cell antigen receptor complex-associated protein alpha chain; CD79A antigen (immunoglobulin-associated alpha); CD79a molecule, immunoglobulin-associated alpha
CD79A 分子靶点综述
CD79a 又称 mb-1,是一种 47kDa 膜糖蛋白,与 CD79b 结合形成异二聚体 B 细胞受体(BCR)。这种受体负责 B 细胞信号传导,导致激活、凋亡或过敏。从最早的B 细胞到浆细胞,CD79a 的表达一直存在。CD79 表达于 B 细胞肿瘤和一些非 B 细胞恶性肿瘤,如 AML。与其它可以在 B 细胞肿瘤细胞中表达的抗体不同,HM47 是识别 CD79a 的可靠抗体,因此是 B 细胞的良好标记。由于 CD79a 的几个变体存在,表位的确切位置可能略有不同。
大鼠 Rat Cd79a 分子靶点信息
- 分子名:Cd79a, CD79a molecule
- 别称:
- -cell antigen receptor complex-associated protein alpha chain-like
- B-cell antigen receptor complex-associated protein alpha chain
- B-cell antigen receptor complex-associated protein alpha chain-like
- CD79A antigen (immunoglobulin-associated alpha)
- Cd79a molecule-like
- Cd79a molecule, immunoglobulin-associated alpha
- Cd79al
- LOC100913063
- 基因序列:NCBI_Gene: 100913063
- 蛋白序列:
大鼠 Rat Cd79a 靶点分子功能(预测)
Predicted to enable identical protein binding activity. Predicted to be involved in B cell differentiation and B cell proliferation. Predicted to act upstream of or within B cell receptor signaling pathway. Predicted to be located in external side of plasma membrane; membrane raft; and multivesicular body. Predicted to be part of B cell receptor complex. Human ortholog(s) of this gene implicated in agammaglobulinemia. Orthologous to human CD79A (CD79a molecule).
操作步骤
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引用文献
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- Long-term cigarette smoking suppresses NLRP3 inflammasome activation in oral mucosal epithelium and attenuates host defense against Candida albicans in a rat model 
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Long-term cigarette smoking suppresses NLRP3 inflammasome activation in oral mucosal epithelium and attenuates host defense against Candida albicans in a rat model 
Orally Deliverable Dual-Targeted Pellets for the Synergistic Treatment of Ulcerative Colitis 
Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 
Accumulative Effects of Multifrequency Microwave Exposure with 1.5 GHz and 2.8 GHz on the Structures and Functions of the Immune System 
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