Rat HGF Standard (大鼠肝细胞生长因子 (HGF) 标准品)
¥120.00
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK3H01
- ELISA试剂盒
Rat HGF ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥2,200.00 – ¥3,700.00
商品名 |
Rat HGF Standard (大鼠肝细胞生长因子 (HGF) 标准品) |
---|---|
组分 |
大鼠肝细胞生长因子 (HGF) 标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠HGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的HGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中HGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
HGF 分子靶点信息概述
- 分子名:HGF, hepatocyte growth factor
- 别名:SF; F-TCF; HGFB; HPTA
- 曾用名:DFNB39
- 全称:hepatopoietin A; fibroblast-derived tumor cytotoxic factor; scatter factor; lung fibroblast-derived mitogen; deafness, autosomal recessive 39; hepatocyte growth factor (hepapoietin A; scatter factor)
HGF 分子靶点综述
肝细胞生长因子(HGF)是旁分泌性细胞生长、运动和形态发生的因子,由间充质细胞分泌产生,可作为大部分上皮来源细胞的多功能细胞因子。HGF以单链形式存在,是一个带有29个氨基酸信号序列和25个氨基酸前体序列的728个氨基酸残基前肽。HGF结合原癌基因c-Met受体后就会激活酪氨酸激酶信号级联,进而调节细胞的生长、运动和形态发生。它在血管生成、肿瘤发生和组织再生中起核心作用。目前正在测试人HGF质粒DNA治疗心肌细胞是否可作为冠状动脉疾病和心肌梗死后心脏损伤的一种潜在治疗。HGF也可作为基孔肯雅病毒诱导的关节痛的预后指示。高水平的HGF与与快速康复有关。
大鼠 Rat Hgf 分子靶点信息
- 分子名:Hgf, hepatocyte growth factor
- 别称:
- hepatocyte growth factor (scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HPTA
- scatter factor
- SF
- 基因序列:NCBI_Gene: 24446
- 蛋白序列:UniProtKB: P17945
大鼠 Rat Hgf 靶点分子功能(预测)
Enables growth factor activity. Involved in several processes, including animal organ regeneration; positive regulation of angiogenesis; and positive regulation of myelination. Located in extracellular space. Used to study brain infarction. Biomarker of cholangiocarcinoma. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
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In vitro study of decellularized rat tissues for nerve regeneration. 
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Mechanisms of Antiulcer Effect of an Active Ingredient Group of Modified Xiao Chaihu Decoction 
Bone marrow mesenchymal stem cells modified with heme oxygenase-1 alleviate rejection of donation after circulatory death liver transplantation by inhibiting dendritic cell maturation in rats 
GRP78 improves the therapeutic effect of mesenchymal stem cells on hemorrhagic shock-induced liver injury: Involvement of the NF–кB and HO-1/Nrf-2 pathways 
Panax quinquefolium L. and Salvia miltiorrhiza Bunge. Enhances Angiogenesis by Regulating the miR-155-5p/HIF-1α/VEGF Axis in Acute Myocardial Infarction 
Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo 
Insulin modified Decellularized Adipose Tissue/Tremella Polysaccharide hydrogel loaded with ADSCs for skin wound healing 
- 细胞培养上清
Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis 
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