Rat C-Reactive Protein/CRP ELISA Kit检测试剂盒（酶联免疫吸附法）
¥1,600.00 – ¥2,650.00
因产品会迭代升级，具体实验步骤请按纸质版说明书操作
- 分子靶点：CRP, C-reactive protein, PTX1
- 种属：大鼠 (Rat)
- 样本类型：血清，血浆，细胞培养上清及其他生物学样本
- 检测样本体积：100 μL
- 灵敏度：3.13 pg/mL
- 检测范围：46.88 pg/mL - 3000 pg/mL
- 回收率：80% - 121%
在售SKU:70-EK394-48, 70-EK394-96
描述
| 商品名 |
Rat C-Reactive Protein/CRP ELISA Kit (大鼠C-反应蛋白 ELISA试剂盒) |
|---|---|
| 检测方法 |
双抗夹心法 |
| 精密度 |
板内变异系数：4.6% - 5.0%；板间变异系数：5.7% - 6.6% |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 |
100 μL |
| 灵敏度 |
3.13 pg/mL |
| 检测范围 |
46.88 pg/mL - 3000 pg/mL |
| 回收率 |
80% - 121% |
| 平均回收率 |
1.08 |
| 板式 |
96孔板，可拆 |
| 保存 |
试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 |
4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠CRP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CRP与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CRP的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CRP 分子靶点信息概述
- 分子名：CRP, C-reactive protein
- 基因家族：Short pentraxins
- 别名：PTX1
- 全称：pentraxin 1; C-reactive protein, pentraxin-related
CRP 分子靶点综述
C-反应蛋白(CRP)是在血浆中发现的一种环状、五聚体蛋白。肝脏对巨噬细胞和脂肪细胞释放的因子产生应答后可合成CRP。CRP是一种肝源性的急性蛋白，在巨噬细胞和T细胞分泌白介素6后增加，其生理作用是与死细胞或濒死细胞(和某些类型的细菌)表面的溶血磷脂胆碱结合，以通过C1Q复合物来激活补体系统。它与C-肽(胰岛素)或蛋白C(血凝)不相关。CRP通常作为炎症标志物，当机体受到炎症感染时，CRP水平会上升。最近的研究表明，患者具有较高基础水平的CRP，通常伴随着一个较高糖尿病、高血压和心血管疾病的发生率。
大鼠 Rat Crp 分子靶点信息
- 分子名：Crp, C-reactive protein
- 别称：
- Aa1249
- Ab1-341
- Ab2-196
- Ac1-114
- Ac1262
- Ac2-069
- Ba2-693
- C-reactive protein member of the pentraxin family
- C-reactive protein, member of the pentraxin family
- C-reactive protein, pentraxin-related
- C-reactive protein, petaxin related
- 基因序列：NCBI_Gene: 25419
- 蛋白序列：
- UniprotKB: A0A0G2K8V5
- UniprotKB: H6X2V2
- UniprotKB: H6X2V3
- UniprotKB: H6X2V4
- UniprotKB: H6X2V7
- UniprotKB: H6X2V8
- UniprotKB: H6X2V9
- UniprotKB: H6X2W0
- UniprotKB: H6X2W1
- UniprotKB: H6X2W2
- UniprotKB: H6X2W3
- UniprotKB: H6X2W4
- UniprotKB: H6X2W5
- UniprotKB: H6X2W6
- UniprotKB: H6X2W7
- UniprotKB: H6X2X0
- UniprotKB: P48199
- UniprotKB: Q63133
- UniprotKB: Q63134
大鼠 Rat Crp 靶点分子功能（预测）
Enables cholesterol binding activity; identical protein binding activity; and low-density lipoprotein particle binding activity. Involved in several processes, including cellular response to nitric oxide; complement activation, classical pathway; and negative regulation of superoxide anion generation. Located in extracellular space; filopodium; and growth cone. Used to study rheumatoid arthritis. Biomarker of several diseases, including acute kidney failure; artery disease (multiple); iron deficiency anemia; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆，哪个是ELISA的菜
- ELISA通关必备丨数据篇丨标准曲线不佳
- ELISA通关必备丨操作篇丨常见问题及解决方案
- ELISA通关必备丨操作篇丨溶解与稀释标准品
- ELISA通关必备丨样本篇丨不常见样本
- ELISA通关必备丨样本篇丨常见样本丨细胞
- ELISA通关必备丨如何选择试剂盒
- ELISA通关必备丨基础知识
- 真？假？ELISA试剂盒选择要小心
- ELISA常见类型一 | 双抗夹心法，你要的都在这里！
- ELISA常见类型二 | 竞争法，五分钟搞定！
- 叮！联科向您投递了个ELISA实验操作干货包，请查收~
- 【视频】ELISA实验操作步骤演示视频教程
- ELISA 组织样本的处理—大鼠组织
- 【视频】ELISA实验原理与常见问题分析
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation
- Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling
- Establishment of a rat model of chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) induced by immunization with a novel peptide T2
- Astragaloside IV Protects from PM2.5-Induced Lung Injury by Regulating Autophagy via Inhibition of PI3K/Akt/mTOR Signaling in vivo and in vitro
- Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway
- Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway
- Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway.
- AMPK/SIRT1 Deficiency Drives Adjuvant-Induced Arthritis in Rats by Promoting Glycolysis-Mediated Monocytes Inflammatory Polarization
- Flos lonicerae flavonoids attenuate experimental ulcerative colitis in rats via suppression of NF-κB signaling pathway
- Allostatic load in rat model: An efficient tool for evaluating and understanding aging
- Zishen Qingre Lishi Huayu Recipe May Ameliorate the Symptoms of PCOS Model Rats via Alleviating Systemic and Ovarian Inflammation
- Implantable Hydrogel-Protective DNA Aptamer-Based Sensor Supports Accurate, Continuous Electrochemical Analysis of Drugs at Multiple Sites in Living Rats
- Inhibition of the MLCK/MLC2 pathway protects against intestinal heat stroke-induced injury in rats
- San Huang Xiao Yan recipe modulates the HMGB1-mediated abnormal inflammatory microenvironment and ameliorates diabetic foot by activating the AMPK/Nrf2 signalling pathway
The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation
Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling
Establishment of a rat model of chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) induced by immunization with a novel peptide T2
Astragaloside IV Protects from PM2.5-Induced Lung Injury by Regulating Autophagy via Inhibition of PI3K/Akt/mTOR Signaling in vivo and in vitro
Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway
Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway
Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway.
AMPK/SIRT1 Deficiency Drives Adjuvant-Induced Arthritis in Rats by Promoting Glycolysis-Mediated Monocytes Inflammatory Polarization
Flos lonicerae flavonoids attenuate experimental ulcerative colitis in rats via suppression of NF-κB signaling pathway
Allostatic load in rat model: An efficient tool for evaluating and understanding aging
Zishen Qingre Lishi Huayu Recipe May Ameliorate the Symptoms of PCOS Model Rats via Alleviating Systemic and Ovarian Inflammation
Implantable Hydrogel-Protective DNA Aptamer-Based Sensor Supports Accurate, Continuous Electrochemical Analysis of Drugs at Multiple Sites in Living Rats
Inhibition of the MLCK/MLC2 pathway protects against intestinal heat stroke-induced injury in rats
San Huang Xiao Yan recipe modulates the HMGB1-mediated abnormal inflammatory microenvironment and ameliorates diabetic foot by activating the AMPK/Nrf2 signalling pathway
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