EK395

Rat Adiponectin/Acrp30 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:ADIPOQ, ACRP30, GBP28
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:0.46 pg/mL
  • 检测范围:78.13 pg/mL - 5000 pg/mL
  • 回收率:80% - 110%

在售SKU:70-EK395-48, 70-EK395-96


描述

商品名

Rat Adiponectin/Acrp30 ELISA Kit (大鼠脂联素 (Adiponectin/Acrp30) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:1.6% - 4.6%;板间变异系数:1.6% - 3.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

0.46 pg/mL

检测范围

78.13 pg/mL - 5000 pg/mL

回收率

80% - 110%

平均回收率

1.05

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Adiponectin单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠Adiponectin冻干标准品
  • 大鼠Adiponectin检测抗体
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(22×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠Adiponectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的Adiponectin与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Adiponectin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 632 nm)测定吸光度值。

Rat Adiponectin/Acrp30 ELISA Kit (大鼠脂联素 (Adiponectin/Acrp30) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 ADIPOQ, ACRP30, GBP28 靶点信息

ADIPOQ 分子靶点信息概述

  • 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
  • 基因家族:C1q and TNF related
  • 别名:ACRP30; apM1; GBP28; adiponectin
  • 曾用名:ACDC
  • 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing

ADIPOQ 分子靶点综述

脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。

大鼠 Rat Adipoq 分子靶点信息

大鼠 Rat Adipoq 靶点分子功能(预测)

Enables identical protein binding activity. Involved in several processes, including cellular response to organonitrogen compound; positive regulation of glycogen (starch) synthase activity; and positive regulation of protein phosphorylation. Located in extracellular space. Part of protein-containing complex. Used to study brain infarction. Biomarker of several diseases, including end stage renal disease; fatty liver disease (multiple); glucose metabolism disease (multiple); hyperthyroidism; and obesity. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).

操作步骤

ELISA操作常见问题

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引用文献


  1. Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 

  2. α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

  3. Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ 

  4. Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling 

  5. Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota 

  6. White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy 

  7. 血清

    Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

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