Rat Adiponectin/Acrp30 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:ADIPOQ, ACRP30, GBP28
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL(稀释液)
- 灵敏度:0.46 pg/mL
- 检测范围:78.13 pg/mL - 5000 pg/mL
- 回收率:80% - 110%
在售SKU:70-EK395-48, 70-EK395-96
描述
商品名 |
Rat Adiponectin/Acrp30 ELISA Kit (大鼠脂联素 (Adiponectin/Acrp30) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:1.6% - 4.6%;板间变异系数:1.6% - 3.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL(稀释液) |
灵敏度 |
0.46 pg/mL |
检测范围 |
78.13 pg/mL - 5000 pg/mL |
回收率 |
80% - 110% |
平均回收率 |
1.05 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠Adiponectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的Adiponectin与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Adiponectin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 632 nm)测定吸光度值。
分子信息
ADIPOQ 分子靶点信息概述
- 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
- 基因家族:C1q and TNF related
- 别名:ACRP30; apM1; GBP28; adiponectin
- 曾用名:ACDC
- 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing
ADIPOQ 分子靶点综述
脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。
大鼠 Rat Adipoq 分子靶点信息
- 分子名:Adipoq, adiponectin, C1Q and collagen domain containing
- 别称:
- Acdc
- Acrp30
- Adid
- adipocyte complement related protein of 30 kDa
- adiponectin
- adiponectin d
- 基因序列:NCBI_Gene: 246253
- 蛋白序列:
大鼠 Rat Adipoq 靶点分子功能(预测)
Enables identical protein binding activity. Involved in several processes, including cellular response to organonitrogen compound; positive regulation of glycogen (starch) synthase activity; and positive regulation of protein phosphorylation. Located in extracellular space. Part of protein-containing complex. Used to study brain infarction. Biomarker of several diseases, including end stage renal disease; fatty liver disease (multiple); glucose metabolism disease (multiple); hyperthyroidism; and obesity. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 
- α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 
- Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ 
- Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling 
- Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota 
- White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy 
- Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 
Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 
α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 
Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ 
Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling 
Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota 
White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy 
- 血清
Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 
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