EK8103

Prostaglandin E2/PGE2 Competitive ELISA Kit 检测试剂盒（酶联免疫吸附法）

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¥2,500.00 – ¥4,000.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 种属：人 (Human); 小鼠 (Mouse); 大鼠 (Rat)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：100 μL(稀释液)
  • 灵敏度：1.08 pg/mL
  • 检测范围：15.63 pg/mL - 2000 pg/mL
  • 回收率：92 - 102%

在售SKU:70-EK8103/2-48, 70-EK8103/2-96

描述

商品名

Prostaglandin E2/PGE2 Competitive ELISA Kit (前列腺素E2 竞争法ELISA试剂盒)
检测方法

竞争法
精密度

板内变异系数：3.9% - 4.3%；板间变异系数：5.2% - 6.5%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

100 μL(稀释液)
灵敏度

1.08 pg/mL
检测范围

15.63 pg/mL - 2000 pg/mL
回收率

92 - 102%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，-20℃保存。已拆开，标准品、抗体、偶联物和酶-20℃保存，其它4℃保存。
运输条件

干冰运输
组分
  • 包被抗小鼠IgG的96孔聚苯乙烯酶标板
  • PGE2冻干标准品
  • PGE2-biotin偶联物冻干粉
  • PGE2抗体冻干粉
  • 辣根过氧化物酶标记的链霉亲和素冻干粉
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用固相竞争酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。PGE2特异性的单克隆抗体加入至孔中，经过孵育，与固相抗体结合。洗涤后，加入生物素标记的PGE2和未标记的PGE2或样本，竞争结合单克隆抗体上有限的结合位点。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物，颜色反应的深浅与PGE2的浓度成反比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本，但其它哺乳动物的PGE2预计也可被检测。

Prostaglandin E2/PGE2 Competitive ELISA Kit (前列腺素E2 ELISA试剂盒) - 标准曲线
标准曲线

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats 

  2. The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells 

  3. Anti-inflammatory activity of Anchusa italica Retz. in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways 

  4. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro 

  5. Decreased Expression of EZH2 in Granulosa Cells Contributes to Endometriosis-Associated Infertility by Targeting IL-1R2 

  6. The estrogen‑like protective effect of Lycium barbarum polysaccharides in reducing oxidative stress on myocardial cells from ovariectomized rats 

  7. Effect of MC3T3 cell density on osteoclastic differentiation of mouse bone marrow cells 

  8. Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation 

  9. P120-Catenin Mediates Intermittent Cyclic Mechanical Tension-Induced Inflammation in Chondrocytes 

  10. Mechanisms of Antiulcer Effect of an Active Ingredient Group of Modified Xiao Chaihu Decoction 

  11. Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats 

  12. Celastrol ameliorates osteoarthritis via regulating TLR2/NF-κB signaling pathway. 

  13. Flos lonicerae flavonoids attenuate experimental ulcerative colitis in rats via suppression of NF-κB signaling pathway 

  14. Protective role of ergothioneine isolated from Pleurotus ostreatus against dextran sulfate sodium-induced ulcerative colitis in rat model 

  15. miR-370-3p Alleviates Ulcerative Colitis-Related Colorectal Cancer in Mice Through Inhibiting the Inflammatory Response and Epithelial-Mesenchymal Transition 

  16. Fucoxanthinol from the Diatom Nitzschia Laevis Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia 

  17. Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro 

  18. Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD 

  19. Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury 

  20. Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers 

  21. Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult 

  22. Compositions and Biological Activities of Pomegranate Peel Polyphenols Extracted by Different Solvents 

  23. Depression Induced by CUMS Leads to Bladder Cancer Development and Local Tumor Immunosuppression in Mice 

  24. Pou3f1 mediates the effect of Nfatc3 on ulcerative colitis-associated colorectal cancer by regulating inflammation 

  25. Polyphenols extracted from Enteromorpha clathrata alleviates inflammation in lipopolysaccharide-induced RAW 264.7 cells by inhibiting the MAPKs/NF-κB signaling pathways 

  26. Systems Pharmacology Based Strategy for Q-Markers Discovery of HuangQin Decoction to Attenuate Intestinal Damage 

  27. Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model 

  28. Knockdown of LINC01385 inhibits osteoarthritis progression by modulating the microRNA‑140‑3p/TLR4 axis 

  29. Intracellular Lipid Accumulation Drives the Differentiation of Decidual Polymorphonuclear Myeloid-Derived Suppressor Cells via Arachidonic Acid Metabolism 

  30. Aconiti Lateralis Radix Praeparata lipid-soluble alkaloids alleviates IL-1β-induced inflammation of human fibroblast-like synoviocytes in rheumatoid arthritis by inhibiting NF-κB and MAPKs signaling pathways and inducing apoptosis 

  31. Effects of dietary fat on gut microbiota and faecal metabolites, and their relationship with cardiometabolic risk factors: a 6-month randomised controlled-feeding trial 

  32. Inhibitory Effect of Cayratia albifolia C. L. Li Extract on LPS-induced Inflammation of RAW264.7 Cells by Regulating the Nrf2/HO-1 Axis 

  33. Blocking CX3CR1+ Tumor-associated Macrophages Enhances the Efficacy of Anti-PD-1 Therapy in Hepatocellular Carcinoma 

  34. Self-delivery nanodrug to manipulate tumor microenvironment for boosting photodynamic cancer immunotherapy 

  35. A Pair of Epimers of Lignan Alleviate Neuroinflammatory Effects by Modulating iNOS/COX-2 and MAPK/NF-κB Signaling Pathways 

  36. Dual depletion of myeloid-derived suppressor cells and tumor cells with self-assembled gemcitabine-celecoxib nano-twin drug for cancer chemoimmunotherapy 

  37. Dexamethasone-Integrated Mesenchymal Stem Cells for Systemic Lupus Erythematosus Treatment via Multiple Immunomodulatory Mechanisms 

  38. Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice 

  39. Mesenchymal stem cells derived from CHIR99021 and TGF?β induction remained on the colicomentum and improved cardiac function of a rat model of acute myocardium infarction 

  40. Mitochondrial-Targeted CS@KET/P780 Nanoplatform for Site-Specific Delivery and High-Efficiency Cancer Immunotherapy in Hepatocellular Carcinoma 

  41. Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres 

  42. Components study on gastroprotective effect and holistic mechanism of the herbal pair Alpinia officinarum - Cyperus rotundus based on spectrum-effect relationship and integrated transcriptome and metabolome analyses 

  43. Tumor Microenvironment Sequential Drug/Gene Delivery Nanosystem for Realizing Multistage Boosting of Cancer–Immunity Cycle on Cancer Immunotherapy 

  44. Involvement of estrogen receptor activation in kaempferol-3-O-glucoside's protection against aging-related cognition impairment and microglial inflammation 

  45. Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1 

  46. Histological evaluation of mouse tongue incisions after Er:YAG laser surgery with different pulse energies versus after conventional scalpel surgery 

  47. Glutaredoxin-1 promotes lymphangioleiomyomatosis progression through inhibiting Bim-mediated apoptosis via COX2/PGE2/ERK pathway 

  48. CircTBX5 knockdown modulates the miR-558/MyD88 axis to alleviate IL-1β-induced inflammation, apoptosis and extracellular matrix degradation in chondrocytes via inactivating the NF-κB signaling 

  49. Thymopentin (TP-5) prevents lipopolysaccharide-induced neuroinflammation and dopaminergic neuron injury by inhibiting the NF-κB/NLRP3 signaling pathway 

  50. Phytochemical composition, antioxidant, anti-tyrosinase, anti-cholinesterase, and anti-inflammatory activities of Rhynchanthus beesianus rhizome extracts 

  51. The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia 

  52. Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression 

  53. Gastroprotective effect of the Lachnum polysaccharide and polysaccharide-dipeptide conjugates against gastric ulcer 

  54. 4-Iodo-6-phenylpyrimidine (4-IPP) suppresses fibroblast-like synoviocyte- mediated inflammation and joint destruction associated with rheumatoid arthritis 

  55. 组织匀浆 细胞培养上清

    Protective Effects of Chitosan-Bilirubin Nanoparticles against Ethanol-Induced Gastric Ulcers 

  56. 组织匀浆 结肠

    Protective role of ergothioneine isolated from Pleurotus ostreatus against dextran sulfate sodium-induced ulcerative colitis in rat model 

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