EK283S

Mouse VEGF Standard (小鼠血管内皮生长因子 标准品)

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¥180.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：VEGFA, VEGF, VPF
  • 种属：小鼠 (Mouse)
  • 试剂盒：EK283
  • 保存：短期4℃保存，长期-20℃保存
  • 运输条件：4℃蓝冰运输

在售SKU:70-EK283S

描述

本产品只包含标准品试剂，如需购买试剂盒请点击下图

商品名

Mouse VEGF Standard (小鼠血管内皮生长因子 标准品)
组分

小鼠VEGF 标准品
检测方法

双抗夹心法
样本类型

血清，血浆，细胞培养上清及其他生物学样本
板式

管
保存

短期4℃，长期-20℃保存
运输条件

4℃蓝冰运输

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的VEGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse VEGF ELISA Kit (小鼠血管内皮生长因子 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

  • 分子名：VEGFA, vascular endothelial growth factor A
  • 基因家族：VEGF family
  • 别名：VEGF-A; VPF
  • 曾用名：VEGF
  • 全称：vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。

小鼠 Mouse Vegfa 分子靶点信息

小鼠 Mouse Vegfa 靶点分子功能（预测）

Enables extracellular matrix binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including generation of neurons; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including alimentary system; brain; egg cylinder; genitourinary system; and heart. Used to study DiGeorge syndrome; amyotrophic lateral sclerosis type 1; newborn respiratory distress syndrome; and psoriasis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. The multiple organs insult and compensation mechanism in mice exposed to hypobaric hypoxia 

  2. Uniformly Sized Stem Cell Spheroids for Treatment of Hind Limb Ischemia: Size Effect 

  3. Inhibition of dipeptidyl peptidase IV prevents high fat diet-induced liver cancer angiogenesis by downregulating chemokine ligand 2 

  4. Hypoxia inducible factor-1 signaling pathway in macrophage involved angiogenesis in materials-instructed osteo-induction 

  5. Melatonin enhances autologous adipose-derived stem cells to improve mouse ovarian function in relation to the SIRT6/NF-κB pathway 

  6. Cytokine Signature Induced by SARS-CoV-2 Spike Protein in a Mouse Model 

  7. Fullerenols boosting the therapeutic effect of anti-CD47 antibody to trigger robust anti-tumor immunity by inducing calreticulin exposure 

  8. The coupling of reduced type H vessels with unloading-induced bone loss and the protection role of Panax quinquefolium saponin in the male mice 

  9. GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris 

  10. Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice 

  11. Aucubin Impeded Preosteoclast Fusion and Enhanced CD31hi EMCNhi Vessel Angiogenesis in Ovariectomized Mice 

  12. Three-Dimensional Culture Decreases the Angiogenic Ability of Mouse Macrophages. 

  13. 17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice 

  14. Fabrication of Biomolecule-Loaded Composite Scaffolds Carried by Extracellular Matrix Hydrogel 

  15. The Intracellular Interaction of Porcine β-Defensin 2 with VASH1 Alleviates Inflammation via Akt Signaling Pathway 

  16. Systemic pharmacological verification of Salvia miltiorrhiza-Ginseng Chinese herb pair in inhibiting spontaneous breast cancer metastasis 

  17. Alkaloid derivative ION-31a inhibits breast cancer metastasis and angiogenesis by targeting HSP90α 

  18. MicroRNA-155 Inhibits Polarization of Macrophages to M2-Type and Suppresses Choroidal Neovascularization 

  19. Intramyocardial injected human umbilical cord-derived mesenchymal stem cells (HucMSCs) contribute to the recovery of cardiac function and the migration of CD4+ T cells into the infarcted heart via CCL5/CCR5 signaling 

  20. Inhibition of Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase-2 Facilitates CD31hiEndomucinhi Blood Vessel and Bone Formation in Ovariectomized Mice 

  21. Macrophage Spheroids with Chronological Phenotype Shifting To Promote Therapeutic Angiogenesis in Critical Limb Ischemia 

  22. Macrophage polarization plays roles in bone formation instructed by calcium phosphate ceramics 

  23. Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice 

  24. Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis 

  25. Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract 

  26. Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD 

  27. Yifei Tongluo, a Chinese Herbal Formula, Suppresses Tumor Growth and Metastasis and Exerts Immunomodulatory Effect in Lewis Lung Carcinoma Mice 

  28. Bu-Shen-Yi-Qi formula ameliorates airway remodeling in murine chronic asthma by modulating airway inflammation and oxidative stress in the lung 

  29. Amelioration of diabetic retinopathy in db/db mice by treatment with different proportional three active ingredients from Tibetan medicine Berberis dictyophylla F 

  30. Exosomes laden self-healing injectable hydrogel enhances diabetic wound healing via regulating macrophage polarization to accelerate angiogenesis 

  31. Effects of combined traffic noise on the synaptic ultrastructure and expressions of p-CaMKII and NMDAR1 in the hippocampus of young SD rats 

  32. Amelioration of dry eye syndrome in db/db mice with diabetes mellitus by treatment with Tibetan Medicine Formula Jikan Mingmu Drops 

  33. Low-fouling granular hydrogel for efficient preparation and delivery of stem cell spheroids towards wound treatment 

  34. Green Tea Derivative Driven Smart Hydrogels with Desired Functions for Chronic Diabetic Wound Treatment 

  35. Macrophage Polarization Mediated by Chitooligosaccharide (COS) and Associated Osteogenic and Angiogenic Activities 

  36. ACSL4 promotes colorectal cancer and is a potential therapeutic target of emodin 

  37. Mesenchymal stromal cells pretreated with proinflammatory cytokines enhance skin wound healing via IL-6-dependent M2 polarization 

  38. Helicobacter pylori induces gastric cancer via down-regulating miR-375 to inhibit dendritic cell maturation 

  39. Natural Flavonoid-Derived Enzyme Mimics DHKNase Balance the Two-Edged Reactive Oxygen Species Function for Wound Healing and Inflammatory Bowel Disease Therapy. 

  40. Engineering an energy-dissipating hybrid tissue in?vivo for obesity treatment 

  41. Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration 

  42. All-in-one hydrogel patches with sprayed bFGF-loaded GelMA microspheres for infected wound healing studies 

  43. TMEM9 promotes lung adenocarcinoma progression via activating the MEK/ERK/STAT3 pathway to induce VEGF expression 

  44. Hamburger-like biomimetic nutrient periosteum with osteoimmunomodulation, angio-/osteo-genesis capacity promoted critical-size bone defect repair 

  45. Brucea javanica derived exosome-like nanovesicles deliver miRNAs for cancer therapy 

  46. Hollow Copper Sulfide Photothermal Nanodelivery Platform Boosts Angiogenesis of Diabetic Wound by Scavenging Reactive Oxygen Species 

  47. Blocking tumor-platelet crosstalk to prevent tumor metastasis via reprograming glycolysis using biomimetic membrane-hybridized liposomes 

  48. Hollow CuS immobilized in polysaccharide hydrogel promotes photothermally-driven vascularization 

  49. Salvianolic acid B promotes angiogenesis and inhibits cardiomyocyte apoptosis by regulating autophagy in myocardial ischemia 

  50. Ultrasmall Calcium-enriched Prussian Blue Nanozymes Promote Chronic Wound Healing by Remodeling Wound Microenvironment 

  51. Tumor Microenvironment Sequential Drug/Gene Delivery Nanosystem for Realizing Multistage Boosting of Cancer–Immunity Cycle on Cancer Immunotherapy 

  52. Umbilical cord-derived mesenchymal stem cells cultured in the MCL medium for aplastic anemia therapy 

  53. Red blood cell-mimicking liposomes loading curcumin promote diabetic wound healing 

  54. Co-Delivery Nanomicelles for Potentiating TNBC Immunotherapy by Synergetically Reshaping CAFs-Mediated Tumor Stroma and Reprogramming Immunosuppressive Microenvironment 

  55. Synchronous targeted delivery of TGF-β siRNA to stromal and tumor cells elicits robust antitumor immunity against triple-negative breast cancer by comprehensively remodeling the tumor microenvironment 

  56. Surface bisphosphonation of polyetheretherketone to manipulate immune response for advanced osseointegration 

  57. Anticoagulants Enhance Molecular and Cellular Immunotherapy of Cancer by Improving Tumor Microcirculation Structure and Function and Redistributing Tumor Infiltrates 

  58. Liposome-anchored mesenchymal stem cells for radiation pneumonia/fibrosis treatment 

  59. Endothelial Stat3 activation promotes osteoarthritis development 

  60. Knockdown of AK142426 suppresses M2 macrophage polarization and inflammation in peritoneal fibrosis via binding to c-Jun 

  61. Reprogramming tumor microenvironment via dual targeting co-delivery of regorafenib and alpha-difluoromethylornithine in osteosarcoma 

  62. P-Hydroxylcinnamaldehyde induces tumor-associated macrophage polarization toward the M1 type by regulating the proteome and inhibits ESCC in vivo and in vitro 

  63. The histologic reaction and permanence of hyaluronic acid gel, calcium hydroxylapatite microspheres, and extracellular matrix bio gel 

  64. Targeted VEGFA therapy in regulating early acute kidney injury and late fibrosis 

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