Mouse VEGF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:VEGFA, VEGF, VPF
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
- 灵敏度:1.97 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:89% - 110%
在售SKU:70-EK283/2-48, 70-EK283/2-96
描述
商品名 |
Mouse VEGF ELISA Kit (小鼠血管内皮生长因子 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.8% - 7.6%;板间变异系数:2.3% - 5.2% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
血清血浆:20 μL;细胞培养上清:100 μL |
灵敏度 |
1.97 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
89% - 110% |
平均回收率 |
0.98 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的VEGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
VEGFA 分子靶点信息概述
- 分子名:VEGFA, vascular endothelial growth factor A
- 基因家族:VEGF family
- 别名:VEGF-A; VPF
- 曾用名:VEGF
- 全称:vascular endothelial growth factor
VEGFA 分子靶点综述
血管内皮生长因子(VEGF)是生长因子的亚家族,具体来说,属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白,参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时,可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期,VEGF调控内皮细胞的增殖、迁移和存活,从而调控血管密度和大小,但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时,实体肿瘤的大小受限;而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF,并控制或减缓疾病进程。
小鼠 Mouse Vegfa 分子靶点信息
- 分子名:Vegfa, vascular endothelial growth factor A
- 别称:
- vascular endothelial growth factor
- Vegf
- VEGF-A
- VEGF120
- VEGF164
- VEGF188
- VPF
- 基因序列:NCBI_Gene: 22339
- 蛋白序列:
小鼠 Mouse Vegfa 靶点分子功能(预测)
Enables extracellular matrix binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including generation of neurons; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including alimentary system; brain; egg cylinder; genitourinary system; and heart. Used to study DiGeorge syndrome; amyotrophic lateral sclerosis type 1; newborn respiratory distress syndrome; and psoriasis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).
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