Mouse TSLP ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
- 分子靶点:TSLP
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.45 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:98% - 119%
在售SKU:70-EK265/2-48, 70-EK265/2-96
描述
商品名 |
Mouse TSLP ELISA Kit (小鼠胸腺基质淋巴细胞生成素 (TSLP) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.3% - 3.4%;板间变异系数:3.4% - 7.4% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.45 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
98% - 119% |
平均回收率 |
1.1 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠TSLP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TSLP与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TSLP的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
TSLP 分子靶点信息概述
- 分子名:TSLP, thymic stromal lymphopoietin
TSLP 分子靶点综述
胸腺基质淋巴细胞生成素(TSLP)主要由非造血细胞如成纤维细胞、上皮细胞和不同类型的基质或基质样细胞产生。它主要影响骨髓细胞、诱导单核细胞释放T细胞虏获趋化因子和增强髓样(CD11c+)树突状细胞成熟。研究表明,TSLP还可激活位于表皮的特定树突状细胞亚群—朗格汉斯细胞的成熟。胸腺TSLP激活髓样和浆细胞样(CD123+)树突状细胞会导致调节性T细胞的产生。TSLP通过胸腺基质淋巴细胞受体CRLF2和IL-7Rα链组成的异源二聚体受体复合物产生信号。结合STAT5后就会诱导其发生磷酸化,进而导致上游转录因子的表达。TSLP的表达与许多疾病包括哮喘、炎症性关节炎、特应性皮炎、湿疹及其它过敏状态有关。
小鼠 Mouse Tslp 分子靶点信息
- 分子名:Tslp, thymic stromal lymphopoietin
- 别称:
- 无
- 基因序列:NCBI_Gene: 53603
- 蛋白序列:
小鼠 Mouse Tslp 靶点分子功能(预测)
Enables cytokine activity. Predicted to be involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Predicted to be located in extracellular region. Is expressed in ductus deferens; epididymis; ileum; and testis. Used to study atopic dermatitis; autoimmune hemolytic anemia; cryoglobulinemia; and membranoproliferative glomerulonephritis. Orthologous to human TSLP (thymic stromal lymphopoietin).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
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- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders 
- Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis 
- Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 
- The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of Asthma 
- IL-17D-induced inhibition of DDX5 expression in keratinocytes amplifies IL-36R-mediated skin inflammation 
- Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways 
- Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion 
- Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 
- Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 
- CKBA suppresses mast cell activation via ERK signaling pathway in murine atopic dermatitis 
- Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 
- Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis 
Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders 
Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis 
Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 
The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of Asthma 
IL-17D-induced inhibition of DDX5 expression in keratinocytes amplifies IL-36R-mediated skin inflammation 
Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways 
Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion 
Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 
Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 
CKBA suppresses mast cell activation via ERK signaling pathway in murine atopic dermatitis 
Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 
- 支气管肺泡灌洗液 BALF
Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis 
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