EK2M09

Mouse Total MMP-9 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:MMP9, MMP-9, CLG4B
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:34.90 pg/mL
  • 检测范围:0.16 ng/mL - 10 ng/mL
  • 回收率:83% - 124%

在售SKU:70-EK2M09-48, 70-EK2M09-96


描述

商品名

Mouse Total MMP-9 ELISA Kit (小鼠总基质金属蛋白酶9 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.4% - 3.7%;板间变异系数:2.8% - 4.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

34.90 pg/mL

检测范围

0.16 ng/mL - 10 ng/mL

回收率

83% - 124%

平均回收率

0.95

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Mouse MMP-9单克隆抗体的96孔聚苯乙烯酶标板
  • Mouse MMP-9冻干标准品
  • Mouse MMP-9检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠MMP-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的MMP-9与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MMP-9的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse Total MMP-9 ELISA Kit (小鼠总基质金属蛋白酶9 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 MMP9, MMP-9, CLG4B 靶点信息

MMP9 分子靶点信息概述

  • 分子名:MMP9, matrix metallopeptidase 9
  • 基因家族:M10 matrix metallopeptidases
  • 曾用名:CLG4B
  • 全称:matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

MMP9 分子靶点综述

基质金属蛋白酶9(MMP-9),又名92kDaIV型胶原酶、92kDa明胶酶或者明胶酶B,是一类属于锌-金属蛋白酶家族的酶。MMP-9参与正常生理过程(如胚胎发育、繁殖、血管形成、骨骼发育、伤口愈细胞迁移、学习和记忆)和病理过程(如关节炎、脑出血和肿瘤转移)的细胞外基质降解。MMP-9和弹性蛋白酶似乎是中性粒细胞迁移通过基底膜的调节因子。MMP-9在血管和新生血管形成中起重要作用。它在人类呼吸道上皮愈合过程中被显著上调,并被发现与许多病理过程相关,如癌症、免疫和心血管疾病。在类风湿性关节炎、局灶性脑缺血案例中可发现MMP-9的水平是升高的。它的水平随着特发性房颤的发展而增加。

小鼠 Mouse Mmp9 分子靶点信息

小鼠 Mouse Mmp9 靶点分子功能(预测)

Enables metalloendopeptidase activity. Involved in negative regulation of epithelial cell differentiation involved in kidney development and response to amyloid-beta. Acts upstream of or within several processes, including collagen catabolic process; embryo implantation; and extracellular matrix organization. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; limb mesenchyme; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and lung disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

操作步骤

ELISA操作常见问题

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引用文献

文章目录[隐藏]


  1. GSK-3α/β Activity Negatively Regulates MMP-1/9 Expression to Suppress Mycobacterium tuberculosis Infection 

  2. Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing 

  3. Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants 

  4. HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice 

  5. MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 

  6. Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity 

  7. A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation 

  8. Exploring the mechanism of Sendeng-4 against rheumacid arthritis through integrated serum pharmacochemistry, transcriptomics, and network pharmacology 

  9. Stomatin promotes neutrophil degranulation and vascular leakage in the early stage of severe burn injury via enhancement of the intracellular binding of neutrophil primary granules to F-actin 

  10. Colchicine ameliorates short-term abdominal aortic aneurysms by inhibiting the expression of NLRP3 inflammasome components in mice 

  11. SPINT2 is involved in the proliferation, migration and phenotypic switching of aortic smooth muscle cells: Implications for the pathogenesis of thoracic aortic dissection 

  12. Influence of miR-142-3p on Pulmonary Fibrosis Through Regulation of p53/NF-κB 

  13. Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke 

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