Mouse Th1/Th2 Staining Kit 小鼠辅助性Th1/Th2细胞检测试剂盒
¥1,850.00 – ¥6,940.00
在售SKU:70-KTH201-100, 70-KTH201-25, th1, th2, 染色, Th, 分型, 细胞分型, 免疫分型, 检测, 分型检测, 试剂盒
描述
产品简介
本产品用于检测小鼠抗凝血或脾细胞中，Th1和Th2细胞在T淋巴细胞 (或CD4+辅助T细胞)中的比例。通常所说的Th1、Th2和Th17是指在各种生理与病理条件下有能力分化为Th1、Th2和Th17 的T辅助细胞 (严格意义上是Th0细胞)。静息状态 (即未受任何刺激，如人的正常生理状态)下，Th0分化为Th1、Th2和Th17的能力非常弱，所以外周血中仅含有极少量的Th1、Th2和Th17细胞，这时所能检测到的IFN-γ、IL-4和IL-17A也微乎其微。而当Th细胞受到外界因素 (如刺激素、病原体等)刺激，其中Th0即会向Th1、Th2或Th17分化，具体分化趋向取决于细胞因子的种类。此时，检测到的IFN-γ、IL-4或IL-17A也较多。实验中检测的Th1、Th2和Th17实际上是检测Th细胞对刺激素刺激的反应能力。
产品特点
- 即用型，无需购买其他产品；
- 灵敏度高，特异性强，结果稳定；
- 价格便宜，现货供应。
结果示例
Flow cytometric analysis of Mouse Th1/Th2 Staining Kit. The staining pattern of IFN-γ and IL-4 on resting heparin anticoagulated blood (A0, B0), PBMCs from EDTA anticoagulated blood (C0, D0), splenocytes (E0, F0) and spleen mononuclear cells (G0, H0) of normal ICR mouse. PMA/Ionomycin stimulated heparin anticoagulated blood (A1, B1), PBMCs from EDTA anticoagulated blood (C1, D1), splenocytes (E1, F1) and spleen mononuclear cells (G1, H1) of normal ICR mouse. Dot plot analysis are derived from gated CD3+/CD4+ cells population. Flow cytometry was performed on a Thermo Fisher Attune NxT.
使用Mouse Th1/Th2 Staining Kit进行流式检测。正常ICR小鼠的静息肝素抗凝血(A0, B0)、EDTA抗凝血来源的PBMCs (C0, D0)、脾细胞(E0, F0)和脾单个核细胞(G0, H0)染色IFN-γ和IL-4。正常ICR小鼠的PMA/Ionomycin刺激的肝素抗凝血(A1, B1)、EDTA抗凝血来源的PBMCs (C1, D1) 脾细胞(E1, F1)和脾单个核细胞(G1, H1)染色IFN-γ和IL-4。对CD3+/CD4+细胞进行设门分析。实验在Thermo Fisher公司的Attune NxT流式细胞仪上进行。
试剂盒组分
| 组分 | 编号 | 25Test | 100Test |
| 抗小鼠CD3ε, FITC (克隆号：145-2C11) | AM003E01 | 150 μl | 600 μl |
| 抗小鼠CD4, PerCP- Cy5.5 (克隆号：GK1.5) | AM00407 | 150 μl | 600 μl |
| 抗小鼠IFN-γ, PE (克隆号：XMG1.2) | AM0IF04 | 150 μl | 600 μl |
| 抗小鼠IL-4, APC (克隆号：11B11) | AM0I405 | 150 μl | 600 μl |
| 佛波酯/离子霉素混合物(250×) | CS1001 | 100 μl | 200 μl |
| 布雷非德菌素A/莫能霉素混合物(250×) | CS1002 | 100 μl | 200 μl |
| 固定破膜剂试剂A | GASA | 3 ml | 11 ml |
| 固定破膜剂试剂B | GASB | 3 ml | 11 ml |
| 流式染色缓冲液 (10×) | S1002 | 15 ml | 60 ml |
引用文献统计
该产品被引用的文献总数为：16
- Escherichia coli Heat-Labile Enterotoxin B Subunit Combined with Ginsenoside Rg1 as an Intranasal Adjuvant Triggers Type I Interferon Signaling Pathway and Enhances Adaptive Immune Responses to an Inactivated PRRSV Vaccine in ICR Mice
影响因子：4.422刊物：Vaccines发表日期：2021/3/16 - Analysis of allergen components and identification of bioactivity of HSP70 in pollen of Populus deltoides
影响因子：2.48刊物：Proteome Science发表日期：2021/9/3 - In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis
影响因子：17.694刊物：Nature Communications发表日期：2022/9/28 - Lactobacillus plantarum P9 ameliorates dysfunctional immune and metabolic homeostasis along the gut–liver axis in a nonalcoholic fatty liver disease with low-grade colitis mouse model
影响因子：4.8刊物：Food Bioscience发表日期：2024-07-02 - Jianpi-Tiaoqi decoction inhibits tumour proliferation and lung metastasis in tumour-bearing mice with triple-negative breast cancer
影响因子：2.4刊物：CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY发表日期：2024-06-06 - Effects of lacidophilin in a mouse model of low-grade colitis and nonalcoholic fatty liver disease
影响因子：4.8刊物：Food Bioscience发表日期：2024-05-31 - Comparison of anti-allergic activities of different types of lotus seed resistant starch in OVA-induced mouse model
影响因子：7.7刊物：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES发表日期：2024-05-14 - Modulatory effect of the fermented Polygonatum cyrtonema Hua on the immune homeostasis and gut integrity in a dextran-sulfate-sodium (DSS)-induced colitis model
影响因子：5.1刊物：Food & Function发表日期：2024-02-15 - The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype
影响因子：5.9刊物：Nutrients发表日期：2023-12-19 - Short-term administration of Qipian?, a mixed bacterial lysate, inhibits airway inflammation in ovalbumin-induced mouse asthma by modulating cellular, humoral and neurogenic immune responses
影响因子：6.1刊物：LIFE SCIENCES发表日期：2023-11-25 - Anthocyanin-Rich Butterfly Pea Flower Extract Ameliorating Low-Grade Inflammation in a High-Fat-Diet and Lipopolysaccharide-Induced Mouse Model
影响因子：6.1刊物：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY发表日期：2023-08-01 - Platycodon grandiflorus polysaccharide regulates colonic immunity through mesenteric lymphatic circulation to attenuate ulcerative colitis
影响因子：4.6刊物：Chinese Journal of Natural Medicines发表日期：2023-04-27 - Alpha-linolenic acid improves nasal mucosa epithelial barrier function in allergic rhinitis by arresting CD4+ T cell differentiation via IL-4Rα-JAK2-STAT3 pathway
影响因子：7.9刊物：PHYTOMEDICINE发表日期：2023-04-21 - Mucosa-associated lymphoid tissue lymphoma translocation protein 1 exaggerates multiple organ injury, inflammation, and immune cell imbalance by activating the NF-κB pathway in sepsis
影响因子：5.2刊物：Frontiers in Microbiology发表日期：2023-03-07 - Oral Administration of Lotus-Seed Resistant Starch Protects against Food Allergy
影响因子：5.2刊物：Foods发表日期：2023-02-08 - Pristane cadmium chloride nanoemulsion accelerates the onset of systemic lupus erythematosus in a C57BL/6 mouse model
影响因子：2.6刊物：LUPUS发表日期：2023-02-07
Th/Treg分型检测试剂盒操作常见问题查看更多Th/Treg分型检测试剂盒操作步骤技术文章
查看更多Th/Treg分型检测试剂盒操作步骤技术文章
引用文献
Zhenxing Chen,Qiangqiang YanImmunomodulatory Effects of Hydrolyzed Seawater Pearl Tablet(HSPT) on Th1/Th2 Functionality in a Mice Model of Chronic Obstructive Pulmonary Disease (COPD) Induced by Cigarette Smoke
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