EK289

Mouse sICAM-1/CD54 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:ICAM1, BB2, CD54
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:1.27 pg/mL
  • 检测范围:62.5 pg/mL - 4000 pg/mL
  • 回收率:89% - 112%

在售SKU:70-EK289/2-48, 70-EK289/2-96


描述

商品名

Mouse sICAM-1/CD54 ELISA Kit (小鼠可溶性细胞间粘附分子1 (sICAM-1) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.6% - 4.0%;板间变异系数:4.1% - 5.1%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

1.27 pg/mL

检测范围

62.5 pg/mL - 4000 pg/mL

回收率

89% - 112%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗sICAM-1/CD54单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠sICAM-1/CD54冻干标准品
  • sICAM-1/CD54检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠sICAM-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sICAM-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sICAM-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse sICAM-1/CD54 ELISA Kit (小鼠可溶性细胞间粘附分子1 (sICAM-1) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 ICAM1, BB2, CD54 靶点信息

ICAM1 分子靶点信息概述

  • 分子名:ICAM1, intercellular adhesion molecule 1
  • 基因家族:CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
  • 别名:BB2; CD54
  • 全称:human rhinovirus receptor

ICAM1 分子靶点综述

细胞间粘附分子1(ICAM-1)也称为CD54,是一个几乎无所不在的跨膜糖蛋白,通常表达于内皮细胞和免疫细胞,对白细胞迁移和活化具有关键作用。它与CD11a/CD18或CD11b/CD18型整合素结合,也会被鼻病毒作为受体利用。可溶性细胞间粘附分子1(sICAM-1)促进血管生成,可作为血管内皮细胞活化或受损的指示剂。它也可作为跨膜ICAM-1抑制剂,介导活性如单核细胞对活化的内皮细胞的黏附以及肿瘤细胞对NK细胞介导的溶解作用的敏感性。监测血清中的sICAM-1表达水平可对多种病理状态提供更加详细的视角,如心血管疾病、II型糖尿病、器官移植障碍、氧化应激、腹部脂肪团、高血压、肝脏疾病和某些恶性肿瘤等。

小鼠 Mouse Icam1 分子靶点信息

小鼠 Mouse Icam1 靶点分子功能(预测)

Predicted to enable integrin binding activity. Acts upstream of or within several processes, including T cell antigen processing and presentation; cellular response to glucose stimulus; and cellular response to leukemia inhibitory factor. Located in external side of plasma membrane and immunological synapse. Colocalizes with membrane raft. Is expressed in several structures, including cardiovascular system; liver vasculature; lung; skin; and thymus primordium. Used to study malaria. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; inflammatory bowel disease (multiple); liver cirrhosis; and uveitis (multiple). Orthologous to human ICAM1 (intercellular adhesion molecule 1).

操作步骤

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引用文献


  1. Bioactive Oligopeptides from Ginseng (Panax ginseng Meyer) Suppress Oxidative Stress-Induced Senescence in Fibroblasts via NAD+/SIRT1/PGC-1α Signaling Pathway 

  2. Robustaflavone-4′-dimethyl ether from Selaginella uncinata attenuated lipopolysaccharide-induced acute lung injury via inhibiting FLT3-mediated neutrophil activation 

  3. Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-κB and MAPKs signaling pathways 

  4. miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10 

  5. The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria 

  6. Beneficial Effects of Small-Molecule Oligopeptides Isolated from Panax Ginseng C. A. Meyer on Cellular Fates in Oxidative Stress-Induced Damaged Human Umbilical Vein Endothelial Cells and PC-12 

  7. Lactoferrin ameliorated obesity-induced endothelial dysfunction by inhibiting the Tak1/IL-18/eNOS pathway between PVAT and vascular endothelium 

  8. Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo 

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