EK2137

Mouse PDGF-BB ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:PDGFB
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:1.05 pg/mL
  • 检测范围:23.44 pg/mL - 1500 pg/mL
  • 回收率:92% - 122%

在售SKU:70-EK2137-48, 70-EK2137-96


描述

商品名

Mouse PDGF-BB ELISA Kit (小鼠血小板衍生因子BB (PDGF-BB) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 5.0%;板间变异系数:3.3%-5.1%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

1.05 pg/mL

检测范围

23.44 pg/mL - 1500 pg/mL

回收率

92% - 122%

平均回收率

1.08

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗PDGF-BB单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠PDGF-BB冻干标准品
  • 小鼠PDGF-BB检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠PDGF-BB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的PDGF-BB与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中PDGF-BB的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse PDGF-BB ELISA Kit (小鼠血小板衍生因子BB (PDGF-BB) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 PDGFB 靶点信息

PDGFB 分子靶点信息概述

  • 分子名:PDGFB, platelet derived growth factor subunit B
  • 别名:SSV
  • 曾用名:SIS
  • 全称:oncogene SIS; becaplermin; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor beta polypeptide

PDGFB 分子靶点综述

血小板衍生因子(PDGF)是众多调节细胞生长和分裂的因子之一。从化学结构上来说,PDGF是一种由两条A链(-AA)或两条B链(-BB)或AB链组合(-AB)形成的二聚体糖蛋白。特别是,它在血管生成、已存在的血管组织生长中起着重要作用。不受控制的血管生成是肿瘤的一个特征。肝细胞和不吸收的破骨细胞表达PDGF-BB,产生成骨细胞和骨头,血小板、巨噬细胞和肥大细胞也能产生PDGF-BB。在损伤部位,PDGF-BB促进中性粒细胞和巨噬细胞浸润,发挥清创作用,促进成纤维细胞分泌新的细胞外基质,促进IGF-1介导再上皮化。

小鼠 Mouse Pdgfb 分子靶点信息

小鼠 Mouse Pdgfb 靶点分子功能(预测)

Predicted to enable several functions, including platelet-derived growth factor binding activity; protein dimerization activity; and receptor ligand activity. Involved in several processes, including metanephric glomerular mesangium development; paracrine signaling; and positive regulation of glomerular filtration. Acts upstream of or within several processes, including animal organ development; nervous system development; and regulation of cell migration. Predicted to be located in several cellular components, including basolateral plasma membrane; dendrite; and neuronal cell body. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; female reproductive system; and nervous system. Human ortholog(s) of this gene implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Orthologous to human PDGFB (platelet derived growth factor subunit B).

操作步骤


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引用文献


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  3. Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis 

  4. Lin28b delays vasculature aging by reducing platelet-derived growth factor-beta resistance in senescent vascular smooth muscle cells 

  5. Macrophage Polarization Mediated by Chitooligosaccharide (COS) and Associated Osteogenic and Angiogenic Activities 

  6. Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration 

  7. All-in-one hydrogel patches with sprayed bFGF-loaded GelMA microspheres for infected wound healing studies 

  8. Salvianolic acid B promotes angiogenesis and inhibits cardiomyocyte apoptosis by regulating autophagy in myocardial ischemia 

  9. Icariside Ⅱ attenuates bleomycin-induced pulmonary fibrosis by modulating macrophage polarization 

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