EK2133

Mouse myeloperoxidase/MPO ELISA Kit检测试剂盒（酶联免疫吸附法）

X

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：MPO, Myeloperoxidase
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：4.40 pg/mL
  • 检测范围：62.50 pg/mL - 4000 pg/mL
  • 回收率：91% - 107%

在售SKU:70-EK2133/2-48, 70-EK2133/2-96

描述

商品名

Mouse myeloperoxidase/MPO ELISA Kit (小鼠髓过氧化物酶 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.8% - 5.4%；板间变异系数：3.0% - 4.1%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

4.40 pg/mL
检测范围

62.50 pg/mL - 4000 pg/mL
回收率

91% - 107%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗MPO单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠MPO冻干标准品
  • MPO检测抗体
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 MPO 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的 MPO 与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中 MPO 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Mouse myeloperoxidase/MPO ELISA Kit (小鼠髓过氧化物酶 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 MPO, Myeloperoxidase 靶点信息

MPO 分子靶点信息概述

  • 分子名：MPO, myeloperoxidase

MPO 分子靶点综述

髓过氧化物酶(MPO)是一种过氧化物酶，在人类中由位于17号染色体上的MPO基因编码。它是一种存在于中性粒细胞的嗜苯胺蓝颗粒中的溶酶体蛋白，在脱颗粒过程中释放到细胞外空间。MPO在中性粒细胞中表达最丰富，可产生具有抗菌活性的次卤酸。MPO有血红素色素，导致中性粒细胞中的分泌物呈现绿色，如脓。另外，MPO介导蛋白质亚硝基化、3-氯酪氨酸形成及二酪氨酸交联。MPO缺乏症是一种导致机体免疫缺陷的遗传性疾病。MPO水平的升高会大大加倍增加心血管死亡的风险。也有人认为，MPO在动脉粥样硬化病变发展中、致使斑块不稳定中起着重要作用。

小鼠 Mouse Mpo 分子靶点信息

小鼠 Mouse Mpo 靶点分子功能（预测）

Enables peroxidase activity. Acts upstream of or within several processes, including hypochlorous acid biosynthetic process; removal of superoxide radicals; and response to fungus. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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  2. An anticomplement homogeneous polysaccharide from Hedyotis diffusa attenuates lipopolysaccharide-induced acute lung injury and inhibits neutrophil extracellular trap formation 

  3. Exploring the Mechanism of Indigo Naturalis in the Treatment of Ulcerative Colitis Based on TLR4/MyD88/NF-κB Signaling Pathway and Gut Microbiota 

  4. Protective Effects of Protopanaxatriol Saponins on Ulcerative Colitis in Mouse Based on UPLC-Q/TOF-MS Serum and Colon Metabolomics 

  5. Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota-Dependent Manner 

  6. Sinoacutine inhibits inflammatory responses to attenuates acute lung injury by regulating NF-κB and JNK signaling pathways 

  7. Orally administered mesoporous silica capped with the cucurbit[8]uril complex to combat colitis and improve intestinal homeostasis by targeting the gut microbiota 

  8. Milk phospholipid supplementation mediates colonization resistance of mice against Salmonella infection in association with modification of gut microbiota 

  9. pH/ROS dual-sensitive and chondroitin sulfate wrapped poly (β-amino ester)-SA-PAPE copolymer nanoparticles for macrophage-targeted oral therapy for ulcerative colitis 

  10. Protective Effect of Ethyl Rosmarinate against Ulcerative Colitis in Mice Based on Untargeted Metabolomics 

  11. miR-223 improves intestinal inflammation through inhibiting the IL-6/STAT3 signaling pathway in dextran sodium sulfate-induced experimental colitis 

  12. Broad-Spectrum Reactive Oxygen Species Scavenging and Activated Macrophage-Targeting Microparticles Ameliorate Inflammatory Bowel Disease 

  13. Reactive oxygen species-scavenging hollow MnO2 nanozymes as carriers to deliver budesonide for synergistic inflammatory bowel disease therapy 

  14. Bifidobacterium longum subsp. infantis FJSYZ1M3 ameliorates DSS-induced colitis by maintaining the intestinal barrier, regulating inflammatory cytokines, and modifying gut microbiota 

  15. Oral colon-targeting core–shell microparticles loading curcumin for enhanced ulcerative colitis alleviating efficacy 

  16. Evodiamine Attenuates Experimental Colitis Injury Via Activating Autophagy and Inhibiting NLRP3 Inflammasome Assembly 

  17. Oral Administration of the Antimicrobial Peptide Mastoparan X Alleviates Enterohemorrhagic Escherichia coli–Induced Intestinal Inflammation and Regulates the Gut Microbiota 

  18. Porous COS@SiO2 Nanocomposites Ameliorate Severe Acute Pancreatitis and Associated Lung Injury by Regulating the Nrf2 Signaling Pathway in Mice 

  19. Pretreatment with chitosan oligosaccharides attenuate experimental severe acute pancreatitis via inhibiting oxidative stress and modulating intestinal homeostasis 

  20. Protective Effects of Lentinan Against Lipopolysaccharide-Induced Mastitis in Mice 

  21. Indirubin Treatment of Lipopolysaccharide-Induced Mastitis in a Mouse Model and Activity in Mouse Mammary Epithelial Cells 

  22. Activation of GPR81 Aggravates Remote Organ Injury During Hepatic Ischemia-Reperfusion Injury 

  23. All-small-molecule supramolecular hydrogels assembled from guanosine 5′-monophosphate disodium salt and tobramycin for the treatment of bacterial keratitis 

  24. Strongylocentrotus nudus Eggs Polysaccharide Enhances Macrophage Phagocytosis Against E.coli Infection by TLR4/STAT3 Axis 

  25. Paneth Cells Protect against Acute Pancreatitis via Modulating Gut Microbiota Dysbiosis 

  26. Porous SiO2-coated ultrasmall selenium particles nanospheres attenuate cerulein-induce acute pancreatitis in mice by downregulating oxidative stress 

  27. Intestinal anti-inflammatory effects of fuzi-ganjiang herb pair against DSS-induced ulcerative colitis in mice 

  28. Baitouweng decoction alleviates dextran sulfate sodium-induced ulcerative colitis by suppressing leucine-related mTORC1 signaling and reducing oxidative stress 

  29. Hydrogel–metal-organic-framework hybrids mediated efficient oral delivery of siRNA for the treatment of ulcerative colitis 

  30. Feiyangchangweiyan capsule protects against ulcerative colitis in mice by modulating the OSM/OSMR pathway and improving gut microbiota 

  31. Ilex rotunda Thunb Protects Against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Restoring the Intestinal Mucosal Barrier and Modulating the Oncostatin M/Oncostatin M Receptor Pathway 

  32. The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier 

  33. Glycoproteins From Rabdosia japonica var. glaucocalyx Regulate Macrophage Polarization and Alleviate Lipopolysaccharide-Induced Acute Lung Injury in Mice via TLR4/NF-κB Pathway 

  34. Sanhuang Xiexin decoction ameliorates DSS-induced colitis in mice by regulating intestinal inflammation, intestinal barrier, and intestinal flora 

  35. Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling 

  36. METTL3-Mediated N6-Methyladenosine Modification of Trim59 mRNA Protects Against Sepsis-Induced Acute Respiratory Distress Syndrome 

  37. Gegen Qinlian decoction ameliorates murine colitis by inhibiting the expansion of Enterobacteriaceae through activating PPAR-γ signaling 

  38. pH/ROS Dual-Sensitive Natural Polysaccharide Nanoparticles Enhance “One Stone Four Birds” Effect of Rhein on Ulcerative Colitis 

  39. MiR-363-3p/S1PR1 axis inhibits sepsis-induced acute lung injury via the inactivation of NF-κB signaling 

  40. Dexamethasone-loaded ROS-responsive poly(thioketal) nanoparticles suppress inflammation and oxidative stress of acute lung injury 

  41. Hsa_circRNA_101036 acts as tumor-suppressor in oral squamous cell carcinoma cells via inducing endoplasmic reticulum stress 

  42. Isoorientin ameliorated ulcerative colitis by inhibiting the Galectin-3/NLRP3/IL-1β signaling pathway 

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  45. Neutrophil extracellular traps as a unique target in the treatment of inflammatory pain 

  46. Integrative analysis of network pharmacology and proteomics reveal the protective effect of Xiaoqinglong Decotion on neutrophilic asthma 

  47. Exopolysaccharides Produced by Bifidobacterium longum subsp. longum YS108R Ameliorates DSS-Induced Ulcerative Colitis in Mice by Improving the Gut Barrier and Regulating the Gut Microbiota 

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  52. Novel Applications of Yinhua Miyanling Tablets in Ulcerative Colitis Treatment Based on Metabolomics and Network Pharmacology 

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  60. Cryptotanshinone alleviates lipopolysaccharide and cigarette smoke-induced chronic obstructive pulmonary disease in mice via the Keap1/Nrf2 axis 

  61. Effects of R-salbutamol on the inflammatory response and acute lung injury in endotoxemic mice 

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  64. Anti-Inflammatory Effect of Clostridium Butyricum-Derived Extracellular Vesicles in Ulcerative Colitis: Impact on Host microRNAs Expressions and Gut Microbiome Profiles 

  65. Hesperetin attenuates sepsis-induced intestinal barrier injury by regulating neutrophil extracellular trap formation via the ROS/autophagy signaling pathway 

  66. Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy 

  67. Sirtuin 1 activator alleviated lethal inflammatory injury via promotion of autophagic degradation of pyruvate kinase M2 

  68. Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway 

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