EK297S

Mouse Leptin Standard (小鼠瘦素 标准品)

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¥280.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：LEP, OBS, OB
  • 种属：小鼠 (Mouse)
  • 试剂盒：EK297
  • 保存：短期4℃保存，长期-20℃保存
  • 运输条件：4℃蓝冰运输

在售SKU:70-EK297S 小鼠 瘦素 小鼠瘦素

描述

本产品只包含标准品试剂，如需购买试剂盒请点击下图

商品名

Mouse Leptin Standard (小鼠瘦素 标准品)
组分

小鼠Leptin 标准品
检测方法

双抗夹心法
样本类型

血清，血浆，细胞培养上清及其他生物学样本
板式

管
保存

短期4℃，长期-20℃保存
运输条件

4℃蓝冰运输

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠Leptin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Leptin与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Leptin的浓度成正比

Mouse Leptin ELISA Kit (小鼠瘦素 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 LEP, OBS, OB 靶点信息

LEP 分子靶点信息概述

  • 分子名：LEP, leptin
  • 基因家族：Neuropeptides
  • 曾用名：OBS; OB
  • 全称：leptin (murine obesity homolog); leptin (obesity homolog, mouse)

LEP 分子靶点综述

瘦素是由脂肪细胞分泌的蛋白质类激素，可通过抑制饥饿来调控能量平衡。除了白色脂肪组织可以分泌瘦素外，褐色脂肪、胎盘、卵巢、骨骼肌、胃、乳腺上皮细胞、骨髓和P/D1细胞也能产生瘦素。瘦素和饥饿激素都作用于下丘脑弓状核的受体，以此来调节食欲，获得能量平衡。瘦素的基本功能是调节脂肪储存，但它在其它生理代谢过程中同样发挥作用，然而许多其它功能还未明确。研究表明瘦素与肥胖和减肥有关联。

小鼠 Mouse Lep 分子靶点信息

小鼠 Mouse Lep 靶点分子功能（预测）

Enables DNA binding activity and growth factor activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cytokine production; and positive regulation of signal transduction. Acts upstream of or within several processes, including insulin secretion; monocarboxylic acid metabolic process; and regulation of insulin secretion. Located in cytoplasm and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; central nervous system; early conceptus; and genitourinary system. Used to study abdominal obesity-metabolic syndrome (multiple); fatty liver disease (multiple); obesity; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Orthologous to human LEP (leptin).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Limits to sustained energy intake. XXX. Constraint or restraint? Manipulations of food supply show peak food intake in lactation is constrained 

  2. In vivo self-assembled small RNAs as a new generation of RNAi therapeutics 

  3. Actin related protein 2/3 complex subunit 1 up-regulation in the hypothalamus prevents high-fat diet induced obesity 

  4. Rutin and Its Combination With Inulin Attenuate Gut Dysbiosis, the Inflammatory Status and Endoplasmic Reticulum Stress in Paneth Cells of Obese Mice Induced by High-Fat Diet 

  5. Gut microbiota mediates the anti-obesity effect of calorie restriction in mice 

  6. GPAT3 deficiency alleviates insulin resistance and hepatic steatosis in a mouse model of severe congenital generalized lipodystrophy 

  7. Glucocorticoid exposure affects female fertility by exerting its effect on the uterus but not on the oocyte: lessons from a hypercortisolism mouse model 

  8. Effects of ambient temperatures between 5 and 35 °C on energy balance, body mass and body composition in mice 

  9. Weight-Reducing Effect of Lactobacillus Plantarum ZJUFT17 Isolated from Sourdough Ecosystem 

  10. Fibroblast transdifferentiation promotes conversion of M1 macrophages and replenishment of cardiac resident macrophages following cardiac injury in mice 

  11. Remodeling on adipocytic physiology of organophosphorus esters in mature adipocytes 

  12. Legumain-deficient macrophages regulate inflammation and lipid metabolism in adipose tissues to protect against diet-induced obesity 

  13. Epigallocatechin gallate suppresses mitotic clonal expansion and adipogenic differentiation of preadipocytes through impeding JAK2/STAT3-mediated transcriptional cascades 

  14. SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice 

  15. Chinese chestnut shell polyphenol extract regulates the JAK2/STAT3 pathway to alleviate high-fat diet-induced, leptin-resistant obesity in mice 

  16. CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis 

  17. Rutin and Its Combination with Inulin Attenuate Gut Dysbiosis, the Inflammatory Status and Endoplasmic Reticulum Stress in Paneth Cells of Obese Mice Induced by High-Fat Diet 

  18. Glucocorticoid Exposure Affects Female Fertility by Exerting Its Effect on the Uterus but Not on the Oocyte: Lessons from a Hypercortisolism Mouse Model 

  19. Human Epidermal Growth Factor Receptor-2 Antibodies Enhance the Specificity and Anticancer Activity of Light-Sensitive Doxorubicin-Labeled Liposomes 

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