Mouse IL-33 High Sensitivity Standard (小鼠白细胞介素33高敏 标准品)
¥280.00
因产品会迭代升级，具体实验步骤请按纸质版说明书操作
描述
文章目录[隐藏]
本产品只包含标准品试剂，如需购买试剂盒请点击下图
-
- EK233HS
- ELISA试剂盒, 高敏试剂盒
Mouse IL-33 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）
- ¥2,000.00 – ¥3,400.00
| 商品名 |
Mouse IL-33 High Sensitivity Standard (小鼠白细胞介素33高敏 标准品) |
|---|---|
| 组分 |
小鼠白细胞介素IL-33高敏标准品 |
| 检测方法 |
双抗夹心法 |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 板式 |
管 |
| 保存 |
短期4℃，长期-20℃保存 |
| 运输条件 |
4℃蓝冰运输 |
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-33与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL33 分子靶点信息概述
- 分子名：IL33, interleukin 33
- 基因家族：Interleukins
- 别名：DVS27; DKFZp586H0523; NF-HEV; IL1F11
- 曾用名：C9orf26
- 全称：DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)
IL33 分子靶点综述
白细胞介素33(IL-33)也称为NF-HEV和DVS27，是IL-1家族成员，分子量为30kDa，由多种类型的细胞分泌，包括成纤维细胞、肥大细胞、树突细胞、巨噬细胞、成骨细胞、内皮细胞和上皮细胞。IL-33可诱导辅助性T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞分泌II型细胞因子。IL-33通过与受体ST2和IL-1受体辅助蛋白(IL1RAP)结合，活化可促使极化的Th2细胞分泌II型细胞因子(如IL-5和IL-13)的NF-κB和MAP激酶信号通路中的胞外分子来介导其生物学功能。注射IL-33后，其可在体内诱导II型细胞因子，这被认为可诱导粘膜器官发生严重的病理变化。
小鼠 Mouse Il33 分子靶点信息
- 分子名：Il33, interleukin 33
- 别称：
- 9230117N10Rik
- Il-33
- Il1f11
- NF-HEV
- RIKEN cDNA 9230117N10 gene
- 基因序列：NCBI_Gene: 77125
- 蛋白序列：
小鼠 Mouse Il33 靶点分子功能（预测）
Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆，哪个是ELISA的菜
- ELISA通关必备丨数据篇丨标准曲线不佳
- ELISA通关必备丨操作篇丨常见问题及解决方案
- ELISA通关必备丨操作篇丨溶解与稀释标准品
- ELISA通关必备丨样本篇丨不常见样本
- ELISA通关必备丨样本篇丨常见样本丨细胞
- ELISA通关必备丨如何选择试剂盒
- ELISA通关必备丨基础知识
- 真？假？ELISA试剂盒选择要小心
- ELISA常见类型一 | 双抗夹心法，你要的都在这里！
- ELISA常见类型二 | 竞争法，五分钟搞定！
- 叮！联科向您投递了个ELISA实验操作干货包，请查收~
- 【视频】ELISA实验操作步骤演示视频教程
- ELISA 组织样本的处理—大鼠组织
- 【视频】ELISA实验原理与常见问题分析
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation
- Rhein Suppresses Lung Inflammatory Injury Induced by Human Respiratory Syncytial Virus Through Inhibiting NLRP3 Inflammasome Activation via NF-κB Pathway in Mice
- Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway
- Glial IL-33 signaling through an ST2-to-CXCL12 pathway in the spinal cord contributes to morphine-induced hyperalgesia and tolerance
- Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis
- Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia
- Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo
- Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway
- Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model
- Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
- Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses
- Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury
- Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance
- The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
- Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
- Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines
Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation
Rhein Suppresses Lung Inflammatory Injury Induced by Human Respiratory Syncytial Virus Through Inhibiting NLRP3 Inflammasome Activation via NF-κB Pathway in Mice
Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway
Glial IL-33 signaling through an ST2-to-CXCL12 pathway in the spinal cord contributes to morphine-induced hyperalgesia and tolerance
Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis
Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia
Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo
Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway
Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses
Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury
Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance
The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines
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