EK233

Mouse IL-33 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL33, IL-33, DVS27
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
  • 灵敏度：8.74 pg/mL
  • 检测范围：46.88 pg/mL - 3000 pg/mL
  • 回收率：85% - 117%

在售SKU:70-EK233/2-48, 70-EK233/2-96

描述

商品名

Mouse IL-33 ELISA Kit (小鼠白细胞介素33 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.9% - 8.9%；板间变异系数：4.9% - 8.1%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：20 μL；细胞培养上清：100 μL
灵敏度

8.74 pg/mL
检测范围

46.88 pg/mL - 3000 pg/mL
回收率

85% - 117%
平均回收率

0.98
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-33单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-33冻干标准品
  • IL-33 检测抗体
  • 辣根过氧化物酶标记的链霉亲和素
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-33与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-33 ELISA Kit (小鼠白细胞介素33 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL33, IL-33, DVS27 靶点信息

IL33 分子靶点信息概述

  • 分子名：IL33, interleukin 33
  • 基因家族：Interleukins
  • 别名：DVS27; DKFZp586H0523; NF-HEV; IL1F11
  • 曾用名：C9orf26
  • 全称：DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)

IL33 分子靶点综述

白细胞介素33(IL-33)也称为NF-HEV和DVS27，是IL-1家族成员，分子量为30kDa，由多种类型的细胞分泌，包括成纤维细胞、肥大细胞、树突细胞、巨噬细胞、成骨细胞、内皮细胞和上皮细胞。IL-33可诱导辅助性T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞分泌II型细胞因子。IL-33通过与受体ST2和IL-1受体辅助蛋白(IL1RAP)结合，活化可促使极化的Th2细胞分泌II型细胞因子(如IL-5和IL-13)的NF-κB和MAP激酶信号通路中的胞外分子来介导其生物学功能。注射IL-33后，其可在体内诱导II型细胞因子，这被认为可诱导粘膜器官发生严重的病理变化。

小鼠 Mouse Il33 分子靶点信息

小鼠 Mouse Il33 靶点分子功能（预测）

Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

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【视频】ELISA实验操作步骤演示视频教程 

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查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

  2. Rhein Suppresses Lung Inflammatory Injury Induced by Human Respiratory Syncytial Virus Through Inhibiting NLRP3 Inflammasome Activation via NF-κB Pathway in Mice 

  3. Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway 

  4. Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis 

  5. Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia 

  6. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 

  7. The effect of neonatal maternal separation on short-chain fatty acids and airway inflammation in adult asthma mice 

  8. Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway 

  9. The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of Asthma 

  10. Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model 

  11. Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis 

  12. Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion 

  13. Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses 

  14. Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury 

  15. Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 

  16. The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs) 

  17. Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 

  18. Honokiol suppresses the aberrant interactions between renal resident macrophages and tubular epithelial cells in lupus nephritis through the NLRP3/IL-33/ST2 axis 

  19. Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 

  20. 支气管肺泡灌洗液 BALF 组织细胞

    Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

  21. 支气管肺泡灌洗液 BALF

    Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis 

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