Mouse IL-21 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL21, IL-21
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:0.58 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:85% - 114%
在售SKU:70-EK221/2-48, 70-EK221/2-96
描述
商品名 |
Mouse IL-21 ELISA Kit (小鼠白细胞介素21 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.0% - 5.5%;板间变异系数:3.5% - 6.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
0.58 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
85% - 114% |
平均回收率 |
0.96 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-21抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-21与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-21的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL21 分子靶点信息概述
- 分子名:IL21, interleukin 21
- 基因家族:Interleukins
- 别名:Za11; IL-21
IL21 分子靶点综述
白细胞介素21(IL-21)对免疫细胞具有强烈的调节作用,包括可破坏病毒感染或肿瘤细胞的自然杀伤细胞(NK)和细胞毒性T细胞。该细胞因子可诱导靶细胞分裂/增殖。IL-21表达于人活化的CD4+T细胞,但在其他大多数组织中并没有表达。此外,IL-21在辅助性T细胞亚群Th2和Th17细胞以及滤泡性T细胞中表达上调。而且IL-21还表达于调控这些细胞功能的NKT细胞中。IL-21在免疫炎性疾病中具有病理作用。研究表明存在一个正向自分泌环,可放大并稳定IL-21驱使的、T细胞介导的应答。而且,它还具有抗肿瘤活性,对自身免疫进程具有作用。在临床上,IL-21还可作为潜在的抗肿瘤药物。
小鼠 Mouse Il21 分子靶点信息
- 分子名:Il21, interleukin 21
- 别称:
- 无
- 基因序列:NCBI_Gene: 60505
- 蛋白序列:
小鼠 Mouse Il21 靶点分子功能(预测)
Enables cytokine receptor binding activity. Involved in cellular response to virus; lymphocyte activation involved in immune response; and positive regulation of immunoglobulin production. Acts upstream of or within positive regulation of cytokine production; positive regulation of natural killer cell differentiation; and positive regulation of natural killer cell mediated cytotoxicity. Predicted to be located in extracellular space. Is expressed in ductus deferens; epididymis; and testis. Human ortholog(s) of this gene implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Orthologous to human IL21 (interleukin 21).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
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- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice 
- Adjuvant-free peptide vaccine targeting Clec9a on dendritic cells can induce robust antitumor immune response through Syk/IL-21 axis 
- Modified Gegen Qinlian Decoction Regulates Treg/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota 
- (−)-Epigallocatechin-3-gallate mitigates cyclophosphamide-induced intestinal injury by modulating the tight junctions, inflammation and dysbiosis in mice 
- Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice 
- Juglone Suppresses Inflammation and Oxidative Stress in Colitis Mice 
- Tfh cell-derived small extracellular vesicles exacerbate the severity of collagen-induced arthritis by enhancing B-cell responses 
- Glycyrrhiza polysaccharides may have an antitumor effect in γδT cells through gut microbiota and TLRs/NF-κB pathway in mice 
- Adjuvant-Free Peptide Vaccine Targeting Clec9a on Dendritic Cells Can Induce Robust Antitumor Immune Response through Syk/Il-21 Axis 
Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice 
Adjuvant-free peptide vaccine targeting Clec9a on dendritic cells can induce robust antitumor immune response through Syk/IL-21 axis 
Modified Gegen Qinlian Decoction Regulates Treg/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota 
(−)-Epigallocatechin-3-gallate mitigates cyclophosphamide-induced intestinal injury by modulating the tight junctions, inflammation and dysbiosis in mice 
Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice 
Juglone Suppresses Inflammation and Oxidative Stress in Colitis Mice 
Tfh cell-derived small extracellular vesicles exacerbate the severity of collagen-induced arthritis by enhancing B-cell responses 
Glycyrrhiza polysaccharides may have an antitumor effect in γδT cells through gut microbiota and TLRs/NF-κB pathway in mice 
- 细胞培养上清
Adjuvant-Free Peptide Vaccine Targeting Clec9a on Dendritic Cells Can Induce Robust Antitumor Immune Response through Syk/Il-21 Axis 
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