EK202HS

Mouse IL-2 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL2, IL-2
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.05 pg/mL
  • 检测范围：0.23 pg/mL - 15 pg/mL
  • 回收率：82% - 98%

在售SKU:70-EK202HS-48, 70-EK202HS-96

描述

商品名

Mouse IL-2 High Sensitivity ELISA Kit (小鼠白细胞介素2 (IL-2) 高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.5% - 4.9%；板间变异系数：4.4% - 8.9%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.05 pg/mL
检测范围

0.23 pg/mL - 15 pg/mL
回收率

82% - 98%
平均回收率

0.92
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-2单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-2冻干标准品
  • IL-2检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-2与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-2 High Sensitivity ELISA Kit (小鼠白细胞介素2 (IL-2) 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

  • 分子名：IL2, interleukin 2
  • 基因家族：Interleukins
  • 别名：IL-2; TCGF
  • 全称：T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白，在人类中，由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子，调控对具有免疫功能的白血球(白细胞，通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答，区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能，其受体由3条链组成，分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角，包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

小鼠 Mouse Il2 分子靶点信息

小鼠 Mouse Il2 靶点分子功能（预测）

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).

操作步骤

文章目录[隐藏]

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

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下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. The Traditional Chinese Medicine Fufang Shatai Heji (STHJ) Enhances Immune Function in Cyclophosphamide-Treated Mice 

  2. Signaling pathway underlying splenocytes activation by polysaccharides from Atractylodis macrocephalae Koidz. 

  3. Bisdemethoxycurcumin Attenuated Renal Injury via Activation of Keap1/Nrf2 Pathway in High-Fat Diet-Fed Mice 

  4. Native nanodiscs from blood inhibit pulmonary fibrosis 

  5. Comparison of CD146 +/− mesenchymal stem cells in improving premature ovarian failure 

  6. Microneedle-Assisted, DC-Targeted Codelivery of pTRP-2 and Adjuvant of Paclitaxel for Transcutaneous Immunotherapy 

  7. Immunomodulatory and Antioxidant Effects of Polysaccharides from the Parasitic Fungus Cordyceps kyushuensis 

  8. Proteomic analysis of lysine acetylation reveals that metabolic enzymes and heat shock proteins may be potential targets for DSS-induced mice colitis 

  9. Triterpene Acids of Loquat Leaf Improve Inflammation in Cigarette Smoking Induced COPD by Regulating AMPK/Nrf2 and NFκB Pathways 

  10. Adjuvant effect of two polysaccharides from the petals of Crocus sativus and its mechanisms 

  11. A Checkpoint-Regulatable Immune Niche Created by Injectable Hydrogel for Tumor Therapy 

  12. Loss of fragile site-associated tumor suppressor promotes antitumor immunity via macrophage polarization 

  13. Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection 

  14. Cytokine-induced killer cells-assisted tumor-targeting delivery of Her-2 monoclonal antibody-conjugated gold nanostars with NIR photosensitizer for enhanced therapy of cancer 

  15. Immune-enhancing effects of polysaccharides MLN-1 from by-product of Mai-luo-ning in vivo and in vitro 

  16. Liposomes with cyclic RGD peptide motif triggers acute immune response in mice 

  17. Antitumor efficacy of chimeric antigen receptor T cells against EGFRvIII-expressing glioblastoma in C57BL/6 mice 

  18. Protection against Mycobacterium tuberculosis Infection Offered by a New Multistage Subunit Vaccine Correlates with Increased Number of IFN-γ+IL-2+ CD4+ and IFN-γ+ CD8+ T Cells 

  19. Dioxin-like (DL-) polychlorinated biphenyls induced immunotoxicity through apoptosis in mice splenocytes via the AhR mediated mitochondria dependent signaling pathways 

  20. Codelivered Chemotherapeutic Doxorubicin via a Dual-Functional Immunostimulatory Polymeric Prodrug for Breast Cancer Immunochemotherapy 

  21. Structural characterization of a novel Schisandra polysaccharides and nutritional intervention in immunotoxicity to PCBs 

  22. Effect of the Chinese Medicine YangZheng XiaoJi on Reducing Fatigue in Mice with Orthotopic Transplantation of Colon Cancer 

  23. Yifei Tongluo, a Chinese Herbal Formula, Suppresses Tumor Growth and Metastasis and Exerts Immunomodulatory Effect in Lewis Lung Carcinoma Mice 

  24. Prolonging Survival of Corneal Transplantation by Selective Sphingosine-1-Phosphate Receptor 1 Agonist 

  25. Systemic application of sphingosine 1-phosphate receptor 1 immunomodulator inhibits corneal allograft rejection in mice 

  26. A new antisarcoma strategy: multisubtype heat shock protein/peptide immunotherapy combined with PD-L1 immunological checkpoint inhibitors 

  27. Oxygen-carrying nanoparticle-based chemo-sonodynamic therapy for tumor suppression and autoimmunity activation 

  28. A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice 

  29. Ginseng Stem-Leaf Saponins in Combination with Selenium Promote the Immune Response in Neonatal Mice with Maternal Antibody 

  30. Olaparib Suppresses MDSC Recruitment via SDF1α/CXCR4 Axis to Improve the Anti-tumor Efficacy of CAR-T Cells on Breast Cancer in Mice 

  31. Mucosal immunity of mannose-modified chitosan microspheres loaded with the nontyepable Haemophilus influenzae outer membrane protein P6 in BALB/c mice 

  32. Hepatitis B virus-triggered PTEN/β-catenin/c-Myc signaling enhances PD-L1 expression to promote immune evasion 

  33. Combined Adjuvant of Poly I:C Improves Antitumor Effects of CAR-T Cells 

  34. Characterization of a polysaccharide from Eupolyphaga sinensis walker and its effective antitumor activity via lymphocyte activation 

  35. Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species 

  36. Separable Microneedle Patch to Protect and Deliver DNA Nanovaccines Against COVID-19 

  37. Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway 

  38. Protective Effect of Bergenin against Cyclophosphamide-Induced Immunosuppression by Immunomodulatory Effect and Antioxidation in Balb/c Mice 

  39. In-situ self-assembled vaccine constructed with dual switchable nanotransformer for tumor immunotherapy 

  40. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 

  41. Coexpression of IL7 and CCL21 Increases Efficacy of CAR-T Cells in Solid Tumors without Requiring Preconditioned Lymphodepletion 

  42. A reduction of Syndecan-4 in macrophages promotes atherosclerosis by aggravating the proinflammatory capacity of macrophages 

  43. HMGB1 aggravates lipopolysaccharide-induced acute lung injury through suppressing the activity and function of Tregs 

  44. In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes 

  45. Resveratrol ameliorates atherosclerosis induced by high-fat diet and LPS in ApoE−/− mice and inhibits the activation of CD4+ T cells 

  46. Chicoric Acid Ameliorates Nonalcoholic Fatty Liver Disease via the AMPK/Nrf2/NFκB Signaling Pathway and Restores Gut Microbiota in High-Fat-Diet-Fed Mice 

  47. Platelet-armored nanoplatform to harmonize janus-faced IFN-γ against tumor recurrence and metastasis 

  48. Depression Induced by CUMS Leads to Bladder Cancer Development and Local Tumor Immunosuppression in Mice 

  49. Perdurable PD-1 blockage awakes anti-tumor immunity suppressed by precise chemotherapy 

  50. Lycopene alleviates AFB1-induced immunosuppression by inhibiting oxidative stress and apoptosis in the spleen of mice 

  51. Preparation, structure characterization of carboxymethylated schisandra polysaccharides and their intervention in immunotoxicity to polychlorinated biphenyls 

  52. Exploring the immunopotentiation of Chinese yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles in an ovalbumin vaccine formulation in vivo 

  53. Immunogenicity and protective efficacy induced by an mRNA vaccine encoding gD antigen against pseudorabies virus infection 

  54. Molecular cloning, codon-optimized gene expression, and bioactivity assessment of two novel fungal immunomodulatory proteins from Ganoderma applanatum in Pichia 

  55. Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy 

  56. Alhagi honey polysaccharides attenuate intestinal injury and immune suppression in cyclophosphamide-induced mice 

  57. Therapeutic Effect of Astragalus Polysaccharides on Hepatocellular Carcinoma H22-Bearing Mice 

  58. Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice 

  59. Gegen Qinlian decoction enhances the effect of PD-1 blockade in colorectal cancer with microsatellite stability by remodelling the gut microbiota and the tumour microenvironment 

  60. Mechanisms underlying immune tolerance caused by recombinant Echinococcus granulosus antigens Eg mMDH and Eg10 in dendritic cells 

  61. Integrated Network Pharmacology and Mice Model to Investigate Qing Zao Fang for Treating Sjögren’s Syndrome 

  62. Transdermal delivery system based on heparin-modified graphene oxide for deep transportation, tumor microenvironment regulation, and immune activation 

  63. Amelioration of Inflammatory Cytokines Mix Stimulation: A Pretreatment of CD137 Signaling Study on VSMC 

  64. Recombination of a dual-CAR-modified T lymphocyte to accurately eliminate pancreatic malignancy 

  65. Effects of ceftriaxone-induced intestinal dysbacteriosis on regulatory T cells validated by anaphylactic mice 

  66. Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(d,l-lactic acid) nanospheres 

  67. The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides 

  68. Alleviation of cyclophosphamide-induced immunosuppression in mice by naturally acetylated hemicellulose from bamboo shavings 

  69. Structural characteristics and immune-enhancing activity of fractionated polysaccharides from Athyrium Multidentatum (Doll.) Ching 

  70. Enhanced immunogenicity of the tuberculosis subunit Rv0572c vaccine delivered in DMT liposome adjuvant as a BCG-booster 

  71. Engineered IL-7 Receptor Enhances the Therapeutic Effect of AXL-CAR-T Cells on Triple-Negative Breast Cancer 

  72. Significantly increased anti-tumor activity of carcinoembryonic antigen-specific chimeric antigen receptor T cells in combination with recombinant human IL-12 

  73. Armored Inducible Expression of IL-12 Enhances Antitumor Activity of Glypican-3–Targeted Chimeric Antigen Receptor–Engineered T Cells in Hepatocellular Carcinoma 

  74. Development of T cells carrying two complementary chimeric antigen receptors against glypican-3 and asialoglycoprotein receptor 1 for the treatment of hepatocellular carcinoma 

  75. Isovitexin Inhibits Ginkgolic Acids-Induced Inflammation Through Downregulating SHP2 Activation 

  76. Metabolic Reprogramming of CD4+ T Cells by Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuates Autoimmune Hepatitis Through Mitochondrial Protein Transfer 

  77. A nanobody-guided multifunctional T cell engager promotes strong anti-tumor responses via synergistic immuno-photothermal effects 

  78. Investigating the antiviral activity of Erigeron annuus (L.) pers extract against RSV and examining its active components 

  79. Antibacterial composite protein microneedle loaded with hypoxia-treated exosomes for oral ulcer healing 

  80. Exploring the mechanism of Sendeng-4 against rheumacid arthritis through integrated serum pharmacochemistry, transcriptomics, and network pharmacology 

  81. Combined cryoablation and PD-1 inhibitor synergistically enhance antitumor immune responses in Lewis lung adenocarcinoma mice via the PI3K/AKT/mTOR pathway 

  82. Plant-Derived Exosome-Like Nanovesicles-Created injectable hydrogel for augmented cancer immunotherapy 

  83. In Situ Sprayed Exosome-Cross-Linked Gel as Artificial Lymph Nodes for Postoperative Glioblastoma Immunotherapy 

  84. Glycyrrhiza polysaccharides may have an antitumor effect in γδT cells through gut microbiota and TLRs/NF-κB pathway in mice 

  85. Biomimetic Nanovehicle-Enabled Targeted Depletion of Intratumoral Fusobacterium nucleatum Synergizes with PD-L1 Blockade against Breast Cancer 

  86. Biologically Self-Assembled Tumor Cell-Derived Cancer Nanovaccines as an All-in-One Platform for Cancer Immunotherapy 

  87. Mitochondrial-Targeted CS@KET/P780 Nanoplatform for Site-Specific Delivery and High-Efficiency Cancer Immunotherapy in Hepatocellular Carcinoma 

  88. Construction and expression of Mycobacterium tuberculosis fusion protein SHR3 and its immunogenicity analysis in combination with various adjuvants 

  89. Mogroside V alleviates inflammation response by modulating miR-21-5P/SPRY1 axis 

  90. Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer 

  91. Targeting and cytotoxicity of chimeric antigen receptor T cells grafted with PD1 extramembrane domain 

  92. Paeoniflorin inhibits hepatocellular carcinoma growth by reducing PD-L1 expression 

  93. Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo 

  94. Expression of codon-optimized PDCoV-RBD protein in baculovirus expression system and immunogenicity evaluation in mice 

  95. Synchronous targeted delivery of TGF-β siRNA to stromal and tumor cells elicits robust antitumor immunity against triple-negative breast cancer by comprehensively remodeling the tumor microenvironment 

  96. Vasoactive intestinal peptide exerts therapeutic action by regulating PTEN in a model of Sj?gren's disease 

  97. A Vanadium-Based Nanoplatform Synergizing Ferroptotic-like Therapy with Glucose Metabolism Intervention for Enhanced Cancer Cell Death and Antitumor Immunity 

  98. Natural killer cells strengthen antitumor activity of cisplatin by immunomodulation and ameliorate cisplatin-induced side effects 

  99. Tumor lysates-constructed hydrogel to potentiate tumor immunotherapy 

  100. Alpha-linolenic acid improves nasal mucosa epithelial barrier function in allergic rhinitis by arresting CD4+ T cell differentiation via IL-4Rα-JAK2-STAT3 pathway 

  101. Synergistic Antitumor Effect of Grifola frondose Polysaccharide—Protein Complex in Combination with Cyclophosphamide in H22 Tumor-Bearing Mice 

  102. Effects of Pine Pollen Polysaccharides and Sulfated Polysaccharides on Ulcerative Colitis and Gut Flora in Mice 

  103. Long-Term Immunogenicity and In Vitro Prophylactic Protective Efficacy of M. tuberculosis Fusion Protein DR2 Combined with Liposomal Adjuvant DIMQ as a Boosting Vaccine for BCG 

  104. Universal chimeric Fcγ receptor T cells with appropriate affinity for IgG1 antibody exhibit optimal antitumor efficacy 

  105. Structure of a Pueraria root polysaccharide and its immunoregulatory activity on T and B lymphocytes, macrophages, and immunosuppressive mice 

  106. Radiotherapy-mediated redox homeostasis-controllable nanomedicine for enhanced ferroptosis sensitivity in tumor therapy 

  107. 细胞培养上清 血清

    Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection 

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