Mouse IL-17E/IL-25 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL25, IL-25, IL17E, IL-17E
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:1.22 pg/mL
- 检测范围:62.5 pg/mL - 4000 pg/mL
- 回收率:73% - 99%
在售SKU:70-EK2179/2-48, 70-EK2179/2-96
描述
商品名 |
Mouse IL-17E/IL-25 ELISA Kit (小鼠 IL-17E/IL-25 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.2% - 3.6%;板间变异系数:3.8% - 4.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
1.22 pg/mL |
检测范围 |
62.5 pg/mL - 4000 pg/mL |
回收率 |
73% - 99% |
平均回收率 |
0.83 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-17E抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-17E与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-17E的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL25 分子靶点信息概述
- 分子名:IL25, interleukin 25
- 基因家族:Interleukins
- 别名:IL-25; IL-17E
- 曾用名:IL17E
- 全称:interleukin 17E
IL25 分子靶点综述
白细胞介素17E(IL-17E),又名IL-25,是IL-17家族中的一员,由2型辅助性T细胞(Th2)和肥大细胞分泌产生。它与IL-17序列相似,可诱导NF-κB活化,刺激IL-8的产生。IL-17E和IL-17B是细胞因子受体IL-17RB的配体。小鼠的研究表明,IL-17E可能是一种促炎性细胞因子,有利于Th2型免疫应答。IL-17E诱导多种组织中的其它细胞因子产生,包括IL-4、IL-5和IL-13,从而刺激嗜酸性粒细胞增多。该细胞因子是一种调控肠道免疫的重要分子,参与与胃肠道相关的慢性炎症。IL-17E可杀死某些类型的乳腺癌细胞。
小鼠 Mouse Il25 分子靶点信息
- 分子名:Il25, interleukin 25
- 别称:
- IL-17E
- Il17e
- interleukin 17E
- 基因序列:NCBI_Gene: 140806
- 蛋白序列:
小鼠 Mouse Il25 靶点分子功能(预测)
Enables cytokine activity. Acts upstream of or within several processes, including eosinophil differentiation; response to fungus; and response to nematode. Predicted to be located in extracellular space. Orthologous to human IL25 (interleukin 25).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
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- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Oral berberine ameliorates high-fat diet-induced obesity by activating TAS2Rs in tuft and endocrine cells in the gut 
- Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 
- Isolation, purification and characterization of Pueraria lobata polysaccharide and its effects on intestinal function in cyclophosphamide-treated mice 
- Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance 
- RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein 
- Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis 
- Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 
- Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 
- Alpha-linolenic acid improves nasal mucosa epithelial barrier function in allergic rhinitis by arresting CD4+ T cell differentiation via IL-4Rα-JAK2-STAT3 pathway 
- Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 
Oral berberine ameliorates high-fat diet-induced obesity by activating TAS2Rs in tuft and endocrine cells in the gut 
Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 
Isolation, purification and characterization of Pueraria lobata polysaccharide and its effects on intestinal function in cyclophosphamide-treated mice 
Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance 
RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein 
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis 
Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 
Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 
Alpha-linolenic acid improves nasal mucosa epithelial barrier function in allergic rhinitis by arresting CD4+ T cell differentiation via IL-4Rα-JAK2-STAT3 pathway 
Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 
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