Mouse IL-17A ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL17A, IL17, CTLA8
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.26 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:91% - 115%
在售SKU:70-EK217/2-48, 70-EK217/2-96
描述
商品名 |
Mouse IL-17A ELISA Kit (小鼠白细胞介素17A (IL-17A) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.8% - 5.6%;板间变异系数:4.2% - 8.4% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.26 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
91% - 115% |
平均回收率 |
1.01 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-17A与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL17A 分子靶点信息概述
- 分子名:IL17A, interleukin 17A
- 基因家族:Interleukins
- 别名:IL-17A; IL-17
- 曾用名:CTLA8; IL17
- 全称:cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
IL17A 分子靶点综述
IL-17主要由Th17型细胞产生,是目前已发现的30余种白细胞介素中的一员,按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子,可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子,从而导致炎症的产生。
小鼠 Mouse Il17a 分子靶点信息
- 分子名:Il17a, interleukin 17A
- 别称:
- Ctla-8
- Ctla8
- cytotoxic T lymphocyte-associated protein 8
- IL-17A
- Il17
- interleukin 17
- 基因序列:NCBI_Gene: 16171
- 蛋白序列:
小鼠 Mouse Il17a 靶点分子功能(预测)
Enables protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including defense response to other organism; intestinal epithelial structure maintenance; and positive regulation of gene expression. Acts upstream of or within cellular response to interleukin-1; positive regulation of cytokine production involved in inflammatory response; and positive regulation of transcription by RNA polymerase II. Located in external side of plasma membrane and extracellular space. Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); eye disease (multiple); and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).
引用文献统计
该产品被引用的文献总数为:120
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ELISA试剂盒操作常见问题查看更多ELISA试剂盒操作步骤技术文章
查看更多ELISA试剂盒操作步骤技术文章
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 一文掌握ELISA实验显色判断、数据分析及标曲拟合 
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
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