EK210

Mouse IL-10 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL10, CSIF, TGIF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：5.8 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：86% - 108%

在售SKU:70-EK210/4-48, 70-EK210/4-96

描述

商品名

Mouse IL-10 ELISA Kit (小鼠白细胞介素10 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.4% - 4.2%；板间变异系数：3.9% - 4.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

5.8 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

86% - 108%
平均回收率

0.97
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-10冻干标准品
  • IL-10检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-10 ELISA Kit (小鼠白细胞介素10 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名：IL10, interleukin 10
  • 基因家族：Interleukins
  • 别名：CSIF; TGIF; IL10A; IL-10
  • 全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

小鼠 Mouse Il10 分子靶点信息

小鼠 Mouse Il10 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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  2. RORγt inhibitor SR1001 alleviates acute pancreatitis by suppressing pancreatic IL-17-producing Th17 and γδ-T cells in mice with ceruletide-induced pancreatitis 

  3. S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome 

  4. Enhancing anti-tumor efficacy and immune memory by combining 3p-GPC-3 siRNA treatment with PD-1 blockade in hepatocellular carcinoma 

  5. Melatonin regulates the immune response and improves Sjögren's syndrome-like symptoms in NOD/Ltj Mice. 

  6. Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats 

  7. Aerobic Exercise Improves Pulmonary Fibrosis by Improving Insulin Resistance and Inflammation in Obese Mice 

  8. Catalpol Inhibits Macrophage Polarization and Prevents Postmenopausal Atherosclerosis Through Regulating Estrogen Receptor Alpha 

  9. Fullerenols boosting the therapeutic effect of anti-CD47 antibody to trigger robust anti-tumor immunity by inducing calreticulin exposure 

  10. Phloretin inhibited the pathogenicity and virulence factors against Candida albicans 

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  13. Bortezomib prodrug catalytic nanoreactor for chemo/chemodynamic therapy and macrophage re-education 

  14. Effective antimicrobial activity of Cbf-14, derived from a cathelin-like domain, against penicillin-resistant bacteria 

  15. Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities 

  16. Biocompatible therapeutic albumin/genipin bioglue for postoperative wound adhesion and residual tumor ablation 

  17. The activation effects of fucoidan from sea cucumber Stichopus chloronotus on RAW264.7 cells via TLR2/4-NF-κB pathway and its structure-activity relationship 

  18. ROS-Responsive Boronate-Stabilized Polyphenol–Poloxamer 188 Assembled Dexamethasone Nanodrug for Macrophage Repolarization in Osteoarthritis Treatment 

  19. The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis 

  20. Hydrolyzed Chicken Meat Extract Attenuates Neuroinflammation and Cognitive Impairment in Middle-Aged Mouse by Regulating M1/M2 Microglial Polarization 

  21. Remodeling immune microenvironment in periodontitis using resveratrol liposomes as an antibiotic-free therapeutic strategy 

  22. Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota 

  23. Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota-Dependent Manner 

  24. Regulatory effect of isovitexin on MAPK/NF-κB signal in mice with acute ulcerative colitis 

  25. Drug-Loaded and Anisotropic Wood-Derived Hydrogel Periosteum with Super Antibacterial, Anti-Inflammatory, and Osteogenic Activities 

  26. Ulvan mediated VE cadherin antibody and REDV peptide co-modification to improve endothelialization potential of bioprosthetic heart valves 

  27. Asperosaponin VI protects mice from sepsis by regulating Hippo and Rho signaling pathway 

  28. Dexmedetomidine attenuates lipopolysaccharide-induced inflammation through macrophageal IL-10 expression following α7 nAchR activation 

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  30. Gastrodin Attenuates Lipopolysaccharide-Induced Inflammatory Response and Migration via the Notch-1 Signaling Pathway in Activated Microglia 

  31. Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy 

  32. Sophocarpine Protects Mice from ConA-Induced Hepatitis via Inhibition of the IFN-Gamma/STAT1 Pathway 

  33. Macrophage Spheroids with Chronological Phenotype Shifting To Promote Therapeutic Angiogenesis in Critical Limb Ischemia 

  34. A high-fat, high-protein diet attenuates the negative impact of casein-induced chronic inflammation on testicular steroidogenesis and sperm parameters in adult mice 

  35. Potential mechanism of oral baicalin treating psoriasis via suppressing Wnt signaling pathway and inhibiting Th17/IL-17 axis by activating PPARγ 

  36. Epigallocatechin-3-Gallate Ameliorates Acute Lung Damage by Inhibiting Quorum-Sensing-Related Virulence Factors of Pseudomonas aeruginosa 

  37. Akkermansia muciniphila promotes type H vessel formation and bone fracture healing by reducing gut permeability and inflammation 

  38. M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro 

  39. Alginate/chitosan multilayer films coated on IL-4-loaded TiO2 nanotubes for modulation of macrophage phenotype 

  40. Targeting macrophagic 17β-HSD7 by fenretinide for the treatment of nonalcoholic fatty liver disease 

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  42. Osteoimmune reaction caused by a novel silicocarnotite bioceramic promoting osteogenesis through the MAPK pathway 

  43. TIGIT negatively regulates inflammation by altering macrophage phenotype 

  44. A vessel subtype beneficial for osteogenesis enhanced by strontium-doped sodium titanate nanorods by modulating macrophage polarization 

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  47. The key role of macrophage depolarization in the treatment of COPD with ergosterol both in vitro and in vivo 

  48. Hybrid Biomaterial Initiates Refractory Wound Healing via Inducing Transiently Heightened Inflammatory Responses 

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  55. Tenacissoside H promotes neurological recovery of cerebral ischaemia/reperfusion injury in mice by modulating inflammation and oxidative stress via TrkB pathway 

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  60. Structural characterisation and immunomodulatory activity of polysaccharides from white asparagus skin 

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  83. Protective Effects of Aminooxyacetic Acid on Colitis Induced in Mice with Dextran Sulfate Sodium 

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  85. Protective and predictive role of Mucin1 in sepsis-induced ALI/ARDS 

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