EK201A

Mouse IL-1α ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:IL1A, IL1-α, IL1F1
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:1.68 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:71% - 106%

在售SKU:70-EK201A/2-48, 70-EK201A/2-96


描述

商品名

Mouse IL-1α ELISA Kit (小鼠白细胞介素1 (IL-1) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.7% - 7.7%;板间变异系数:4.3% - 6.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

1.68 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

71% - 106%

平均回收率

0.89

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-1α 单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-1α 冻干标准品
  • IL-1α 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-1α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-1α与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-1α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-1α ELISA Kit (小鼠白细胞介素1 (IL-1) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL1A, IL1-α, IL1F1 靶点信息

IL1A 分子靶点信息概述

  • 分子名:IL1A, interleukin 1 alpha
  • 基因家族:Interleukins
  • 别名:IL1F1; IL-1A; IL1-ALPHA
  • 曾用名:IL1
  • 全称:preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha

IL1A 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1α是一种多效性细胞因子,在人类中由IL1A基因编码。IL-1α主要由活化的巨噬细胞、中性粒细胞、上皮细胞和内皮细胞产生。它具有代谢、生理、造血活性,在调节免疫应答中起到核心作用。IL-1α可与HAX1、NDN相互作用,但是与TNF之间的协同作用是临床上最相关的。IL-1α和TNF都是急性期细胞因子,具有促发热和发炎作用。人们目前正在评估IL-1α是否可作为临床试验中一个潜在治疗肿瘤的标志物。阻断IL-1α活性具有潜在治疗皮肤病的作用,例如痤疮。

小鼠 Mouse Il1a 分子靶点信息

小鼠 Mouse Il1a 靶点分子功能(预测)

Enables cytokine activity and interleukin-1 receptor binding activity. Involved in positive regulation of angiogenesis and positive regulation of vascular endothelial growth factor production. Acts upstream of or within several processes, including cell surface receptor signaling pathway; connective tissue replacement involved in inflammatory response wound healing; and positive regulation of cytokine production. Located in cytoplasm and extracellular space. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; reproductive system; and respiratory system. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).

操作步骤

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引用文献

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