EK272

Mouse IgG1 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

分子靶点：IgG
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：0.02 ng/mL
检测范围：3.13 ng/mL - 200 ng/mL
回收率：96% - 108%

在售SKU:70-EK272-48, 70-EK272-96, 免疫球蛋白, 球蛋白, 免疫球蛋白G, 球蛋白G, IgG, Immunoglobulins, G, 免疫球蛋白1, 球蛋白1, 免疫球蛋白G1, 球蛋白G1, IgG1, Immunoglobulins, G1

描述

商品名	Mouse IgG1 ELISA Kit (小鼠IgG1 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.2%-8.9%；板间变异系数：1.5% - 8.6%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	0.02 ng/mL
检测范围	3.13 ng/mL - 200 ng/mL
回收率	96% - 108%
平均回收率	1.01
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IgG1单克隆抗体的96孔聚苯乙烯酶标板小鼠IgG1冻干标准品 IgG1检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IgG1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的IgG1与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgG1的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IgG1 ELISA Kit (小鼠IgG1 ELISA试剂盒) - 标准曲线 — 标准曲线

操作步骤

文章目录[隐藏]

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引用文献

文章目录[隐藏]

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