EK271

Mouse IgG ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IgG
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：10 μL
  • 灵敏度：10.70 pg/mL
  • 检测范围：6.25 ng/mL - 400 ng/mL
  • 回收率：89% - 101%

在售SKU:70-EK271-48, 70-EK271-96, 免疫球蛋白, 球蛋白, 免疫球蛋白G, 球蛋白G, IgG, Immunoglobulins, G

描述

商品名

Mouse IgG ELISA Kit (小鼠免疫球蛋白G (IgG) ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.2% - 4.8%；板间变异系数：2.4% - 3.0%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

10 μL
灵敏度

10.70 pg/mL
检测范围

6.25 ng/mL - 400 ng/mL
回收率

89% - 101%
平均回收率

0.94
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IgG单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IgG冻干标准品
  • IgG检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IgG抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本进行孵育。经过孵育，样本中存在的IgG与固相抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的检测抗体孵育。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgG的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IgG ELISA Kit (小鼠免疫球蛋白G (IgG) ELISA试剂盒) - 标准曲线
标准曲线

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Exploring the Mechanism of Indigo Naturalis in the Treatment of Ulcerative Colitis Based on TLR4/MyD88/NF-κB Signaling Pathway and Gut Microbiota 

  2. Signaling pathway underlying splenocytes activation by polysaccharides from Atractylodis macrocephalae Koidz. 

  3. Use of immune modulation by human adipose-derived mesenchymal stem cells to treat experimental arthritis in mice 

  4. Microneedle-Assisted, DC-Targeted Codelivery of pTRP-2 and Adjuvant of Paclitaxel for Transcutaneous Immunotherapy 

  5. Comparison of water- and alkali-extracted polysaccharides from Fuzhuan brick tea and their immunomodulatory effects in vitro and in vivo 

  6. Microbiome-metabolome responses of Fuzhuan brick tea crude polysaccharides with immune-protective benefit in cyclophosphamide-induced immunosuppressive mice 

  7. Engineering antiviral immune-like systems for autonomous virus detection and inhibition in mice 

  8. GPX4-Regulated Ferroptosis Mediates S100-Induced Experimental Autoimmune Hepatitis Associated with the Nrf2/HO-1 Signaling Pathway 

  9. Immune-enhancing effects of polysaccharides MLN-1 from by-product of Mai-luo-ning in vivo and in vitro 

  10. Cryptotanshinone ameliorates the pathogenesis of systemic lupus erythematosus by blocking T cell proliferation 

  11. Mannose-Modified PLGA Nanoparticles for Sustained and Targeted Delivery in Hepatitis B Virus Immunoprophylaxis 

  12. Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response 

  13. Condensed Fuzheng extract increases immune function in mice with cyclophosphamide-induced immunosuppression 

  14. Protective Effect of Bergenin against Cyclophosphamide-Induced Immunosuppression by Immunomodulatory Effect and Antioxidation in Balb/c Mice 

  15. Silk Fibroin-Coated Nanoagents for Acidic Lysosome Targeting by a Functional Preservation Strategy in Cancer Chemotherapy 

  16. Immunological regulation of the active fraction from Polygonatum sibiricum F. Delaroche based on improvement of intestinal microflora and activation of RAW264.7 cells 

  17. Anti-tumor and immunomodulation activity of polysaccharides from Dendrobium officinale in S180 tumor-bearing mice 

  18. Zwitterionic Polysulfamide Drug Nanogels with Microwave Augmented Tumor Accumulation and On-Demand Drug Release for Enhanced Cancer Therapy 

  19. DC32, a Dihydroartemisinin Derivative, Ameliorates Collagen-Induced Arthritis Through an Nrf2-p62-Keap1 Feedback Loop 

  20. CBP22, a Novel Bacteriocin Isolated from Clostridium butyricum ZJU-F1, Protects against LPS-Induced Intestinal Injury through Maintaining the Tight Junction Complex 

  21. Effects of Dendrobium officinale ultrafine powder on sub-health mice induced by unhealthy lifestyle based on neuroendocrine immune system 

  22. Intranasal co-administration of 1,8-cineole with influenza vaccine provide cross-protection against influenza virus infection 

  23. Gancao Fuzi decoction regulates the Th17/Treg cell imbalance in rheumatoid arthritis by targeting Foxp3 via miR-34a 

  24. Enhancing Immune Responses to a DNA Vaccine Encoding Toxoplasma gondii GRA7 Using Calcium Phosphate Nanoparticles as an Adjuvant 

  25. Disabled-2 overexpression mediates immune suppression in systemic lupus erythematosus by modulating Treg/Th17 cell differentiation 

  26. CD4+ T Cell-Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses 

  27. Hepatitis B virus-induced hyperactivation of B cells in chronic hepatitis B patients via TLR4 

  28. microRNA-143-3p attenuated development of hepatic fibrosis in autoimmune hepatitis through regulation of TAK1 phosphorylation 

  29. Multi-functional nanocomplex codelivery of Trp2 and R837 to activate melanoma-specific immunity 

  30. BMSCs attenuate hepatic fibrosis in autoimmune hepatitis through regulation of LMO7-AP1-TGFβ signaling pathway 

  31. Inhibition of miR-431-5p attenuated liver apoptosis through KLF15/p53 signal pathway in S100 induced autoimmune hepatitis mice 

  32. TRIM31 facilitates K27-linked polyubiquitination of SYK to regulate antifungal immunity 

  33. Therapeutic effects of cationic liposomes on lupus-prone MRL/lpr mice are mediated via inhibition of TLR4-triggered B-cell activation 

  34. Exploring the immunopotentiation of Chinese yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles in an ovalbumin vaccine formulation in vivo 

  35. CX-5461 ameliorates disease in lupus-prone mice by triggering B-cell ferroptosis via p53-SLC7A11-ALOX12 pathway 

  36. Beneficial effects of Dendrobium officinale National Herbal Drink on metabolic immune crosstalk via regulate SCFAs-Th17/Treg 

  37. Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment 

  38. Artesunate alleviates Sj?gren's Syndrome by inhibiting the interferon-α signaling in plasmacytoid dendritic cells via TLR-MyD88-IRF7 

  39. Bifidobacterium breve CCFM1310 enhances immunity in immunosuppressed mice via modulating immune response and gut microbiota 

  40. Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis 

  41. Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia?reperfusion injury in heart transplantation 

  42. Blockade of Notch1 Signaling Alleviated Podocyte Injury in Lupus Nephritis Via Inhibition of NLRP3 Inflammasome Activation 

  43. Short-term administration of Qipian?, a mixed bacterial lysate, inhibits airway inflammation in ovalbumin-induced mouse asthma by modulating cellular, humoral and neurogenic immune responses 

  44. Angelica sinensis polysaccharides ameliorated 5-Fluorouracil-induced damage of early B cell progenitors by alleviating oxidative stress of IL-7 producing mesenchymal stem and progenitor cells 

  45. Flot2 deficiency facilitates B cell-mediated inflammatory responses and endotoxic shock 

  46. Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offsprings 

  47. Neutrophil-Mediated Tumor-Targeting Delivery System of Oncolytic Bacteria Combined with ICB for Melanoma Lung Metastasis Therapy 

  48. Protective efficacy of Toxoplasma gondii GRA12 or GRA7 recombinant proteins encapsulated in PLGA nanoparticles against acute Toxoplasma gondii infection in mice 

  49. Polymorphonuclear myeloid-derived suppressor cells play a proinflammatory role via TNF-α+ B cells through BAFF/BTK/NF-κB signalling pathway in the pathogenesis of collagen-induced arthritis mice 

  50. Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model 

  51. Effect of bacillus subtilis strain Z15 secondary metabolites on immune function in mice 

  52. Carboxybetaine-Based Zwitterionic Polymer Nanogels with Long Blood Circulation for Cancer Therapy 

  53. Orchestrating antigen delivery and presentation efficiency in lymph node by nanoparticle shape for immune response 

  54. Insight into the structural and immunomodulatory relationships of polysaccharides from Dendrobium officinale-an in vivo study 

  55. Polymeric immunoglobulin receptor deficiency exacerbates autoimmune hepatitis by inducing intestinal dysbiosis and barrier dysfunction 

  56. Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles 

  57. FGF4 improves hepatocytes ferroptosis in autoimmune hepatitis mice via activation of CISD3 

  58. 血清

    Vesicle-Mediated Dendritic Cell Activation in Acinetobacter Baumannii Clinical Isolate, Which Contributes to Th2 Response 

  59. 血清

    microRNA-143-3p Attenuated Development of Hepatic Fibrosis in Autoimmune Hepatitis through Regulation of Tak1 Phosphorylation 

  60. Cd4(+) T Cell-Released Extracellular Vesicles Potentiate the Efficacy of the Hbsag Vaccine by Enhancing B Cell Responses 

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