Mouse GM-CSF High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CSF2, GM-CSF
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.23 pg/mL
- 检测范围:2.5 pg/mL - 160 pg/mL
- 回收率:91% - 112%
在售SKU:70-EK263HS-48, 70-EK263HS-96
描述
商品名 |
Mouse GM-CSF High Sensitivity ELISA Kit (小鼠粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 高敏 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:4.2% - 4.8%;板间变异系数:3.8% - 4.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.23 pg/mL |
检测范围 |
2.5 pg/mL - 160 pg/mL |
回收率 |
91% - 112% |
平均回收率 |
0.98 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠GM-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的GM-CSF与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中GM-CSF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CSF2 分子靶点信息概述
- 分子名:CSF2, colony stimulating factor 2
- 别名:GM-CSF; GMCSF
- 全称:sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)
CSF2 分子靶点综述
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种调节白细胞生长的细胞因子。GM-CSF刺激干细胞产生粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)及单核细胞。此外,它对造血和非造血谱系细胞也具有活性。GM-CSF用作药物来刺激白细胞的产生,从而预防化疗后引起的中性粒细胞减少症。近来,人们正在临床试验评估GM-CSF作为一种潜在的疫苗佐剂对HIV感染患者的疗效。相关研究报道一些癌症(包括肺癌和急性髓细胞白血病)患者的血清中具有较高水平的GM-CSF,同样在支气管哮喘患者外周血和鳞屑提取物中也具有较高水平的GM-CSF。
小鼠 Mouse Csf2 分子靶点信息
- 分子名:Csf2, colony stimulating factor 2 (granulocyte-macrophage)
- 别称:
- colony stimulating factor, granulocyte macrophage
- Csfgm
- Gm-CSf
- GMCSF
- MGI-IGM
- 基因序列:NCBI_Gene: 12981
- 蛋白序列:
小鼠 Mouse Csf2 靶点分子功能(预测)
Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
操作步骤
文章目录[隐藏]
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- 真?假?ELISA试剂盒选择要小心 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota 
- Lipopolysaccharide induced intestinal epithelial injury: a novel organoids-based model for sepsis in vitro 
- Down-regulation of taurine-up-regulated gene 1 attenuates inflammation by sponging miR-9-5p via targeting NF-κB1/p50 in multiple sclerosis 
- A vascular disrupting agent overcomes tumor multidrug resistance by skewing macrophage polarity toward the M1 phenotype 
- Non-peptidyl non-covalent cathepsin C inhibitoEEr bearing a unique thiophene-substituted pyridine: Design, structure-activity relationship and anti-inflammatory activity in vivo 
- Optimized Intracellular Staining Reveals Heterogeneous Cytokine Production Ability of Murine and Human Hematopoietic Stem and Progenitor Cells 
- Reconstituted CD74+ NK cells trigger chronic graft versus host disease after allogeneic bone marrow transplantation 
- New insight into prurigo nodularis: Proadrenomedullin N-terminal 20 peptide mediates mouse mast cell activation via Mrgprb2 
Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota 
Lipopolysaccharide induced intestinal epithelial injury: a novel organoids-based model for sepsis in vitro 
Down-regulation of taurine-up-regulated gene 1 attenuates inflammation by sponging miR-9-5p via targeting NF-κB1/p50 in multiple sclerosis 
A vascular disrupting agent overcomes tumor multidrug resistance by skewing macrophage polarity toward the M1 phenotype 
Non-peptidyl non-covalent cathepsin C inhibitoEEr bearing a unique thiophene-substituted pyridine: Design, structure-activity relationship and anti-inflammatory activity in vivo 
Optimized Intracellular Staining Reveals Heterogeneous Cytokine Production Ability of Murine and Human Hematopoietic Stem and Progenitor Cells 
Reconstituted CD74+ NK cells trigger chronic graft versus host disease after allogeneic bone marrow transplantation 
New insight into prurigo nodularis: Proadrenomedullin N-terminal 20 peptide mediates mouse mast cell activation via Mrgprb2 
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