Mouse GM-CSF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CSF2, GM-CSF
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.65 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:88% - 105%
在售SKU:70-EK263/2-48, 70-EK263/2-96
描述
商品名 |
Mouse GM-CSF ELISA Kit (小鼠粒细胞-巨噬细胞集落刺激因子 (GM-CSF) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.8% - 3.5%;板间变异系数:2.5% - 4.4% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.65 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
88% - 105% |
平均回收率 |
0.95 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠GM-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的GM-CSF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中GM-CSF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CSF2 分子靶点信息概述
- 分子名:CSF2, colony stimulating factor 2
- 别名:GM-CSF; GMCSF
- 全称:sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)
CSF2 分子靶点综述
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种调节白细胞生长的细胞因子。GM-CSF刺激干细胞产生粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)及单核细胞。此外,它对造血和非造血谱系细胞也具有活性。GM-CSF用作药物来刺激白细胞的产生,从而预防化疗后引起的中性粒细胞减少症。近来,人们正在临床试验评估GM-CSF作为一种潜在的疫苗佐剂对HIV感染患者的疗效。相关研究报道一些癌症(包括肺癌和急性髓细胞白血病)患者的血清中具有较高水平的GM-CSF,同样在支气管哮喘患者外周血和鳞屑提取物中也具有较高水平的GM-CSF。
小鼠 Mouse Csf2 分子靶点信息
- 分子名:Csf2, colony stimulating factor 2 (granulocyte-macrophage)
- 别称:
- colony stimulating factor, granulocyte macrophage
- Csfgm
- Gm-CSf
- GMCSF
- MGI-IGM
- 基因序列:NCBI_Gene: 12981
- 蛋白序列:
小鼠 Mouse Csf2 靶点分子功能(预测)
Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
引用文献统计
该产品被引用的文献总数为:10
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影响因子:7.088刊物:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY发表日期:2022/4/10 - Reconstituted CD74+ NK cells trigger chronic graft versus host disease after allogeneic bone marrow transplantation
影响因子:7.9刊物:JOURNAL OF AUTOIMMUNITY发表日期:2024-06-26 - New insight into prurigo nodularis: Proadrenomedullin N-terminal 20 peptide mediates mouse mast cell activation via Mrgprb2
影响因子:2刊物:SKIN RESEARCH AND TECHNOLOGY发表日期:2024-01-29 - Non-peptidyl non-covalent cathepsin C inhibitoEEr bearing a unique thiophene-substituted pyridine: Design, structure-activity relationship and anti-inflammatory activity in vivo
刊物:Eur J Med Chem. 2022 Jun 5;236:114368.
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