Mouse G-CSF Standard (小鼠粒细胞集落刺激因子 标准品)
¥280.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK269
- ELISA试剂盒
Mouse G-CSF ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥2,650.00
商品名 |
Mouse G-CSF Standard (小鼠粒细胞集落刺激因子 标准品) |
---|---|
组分 |
小鼠G-CSF 标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠G-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的G-CSF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中G-CSF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CSF3 分子靶点信息概述
- 分子名:CSF3, colony stimulating factor 3
- 基因家族:Receptor ligands
- 别名:MGC45931
- 曾用名:GCSF; G-CSF; C17orf33
- 全称:granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte)
CSF3 分子靶点综述
粒细胞集落刺激因子(G-CSF)是一种糖蛋白,可刺激骨髓产生粒细胞和干细胞,并把它们释放到血液中。从功能上来说,它是一种细胞因子和激素,是一种集落刺激因子,由内皮细胞、巨噬细胞和许多其它免疫细胞产生。G-CSF刺激中性粒细胞前体和成熟中性粒细胞的生存、增殖、分化及功能发挥。在肿瘤学和血液学上,重组G-CSF用于加速癌症患者化疗后引起的嗜中性白血球减少症的恢复。在白细胞去除前,G-CSF也可用来增加献血者血液中的造血干细胞数量,用于造血干细胞移植。
小鼠 Mouse Csf3 分子靶点信息
- 分子名:Csf3, colony stimulating factor 3 (granulocyte)
- 别称:
- colony stimulating factor, granulocyte
- Csfg
- G-CSF
- MGI-IG
- 基因序列:NCBI_Gene: 12985
- 蛋白序列:
小鼠 Mouse Csf3 靶点分子功能(预测)
Enables granulocyte colony-stimulating factor receptor binding activity. Acts upstream of or within positive regulation of myeloid cell differentiation. Predicted to be located in endocytic vesicle lumen and extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including brain; dorsal aorta; liver; reproductive system; and yolk sac. Human ortholog(s) of this gene implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Orthologous to human CSF3 (colony stimulating factor 3).
操作步骤
文章目录[隐藏]
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
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Locally organised and activated Fth1hi neutrophils aggravate inflammation of acute lung injury in an IL-10-dependent manner 
Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer 
Hypofractionated Irradiation Suppressed the Off-Target Mouse Hepatocarcinoma Growth by Inhibiting Myeloid-Derived Suppressor Cell-Mediated Immune Suppression 
High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid 
Fluorous-Tagged Peptide Nanoparticles Ameliorate Acute Lung Injury via Lysosomal Stabilization and Inflammation Inhibition in Pulmonary Macrophages 
CPT1A-IL-10-mediated macrophage metabolic and phenotypic alterations ameliorate acute lung injury 
Protection of Neutrophils by Bone Marrow Mesenchymal Stromal Cells is Enhanced by Tumor-associated Inflammatory Cytokines 
Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion 
Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells 
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